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B7GQ20 (THIM_BIFLS) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 34. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Hydroxyethylthiazole kinase

EC=2.7.1.50
Alternative name(s):
4-methyl-5-beta-hydroxyethylthiazole kinase
Short name=TH kinase
Short name=Thz kinase
Gene names
Name:thiM
Ordered Locus Names:Blon_0797, BLIJ_0811
OrganismBifidobacterium longum subsp. infantis (strain ATCC 15697 / DSM 20088 / JCM 1222 / NCTC 11817 / S12) [Complete proteome] [HAMAP]
Taxonomic identifier391904 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeBifidobacterialesBifidobacteriaceaeBifidobacterium

Protein attributes

Sequence length314 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole. HAMAP-Rule MF_00228

Cofactor

Magnesium By similarity. HAMAP-Rule MF_00228

Pathway

Cofactor biosynthesis; thiamine diphosphate biosynthesis; 4-methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-methylthiazole: step 1/1. HAMAP-Rule MF_00228

Sequence similarities

Belongs to the Thz kinase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 314314Hydroxyethylthiazole kinase HAMAP-Rule MF_00228
PRO_0000383825

Sites

Binding site701Substrate; via amide nitrogen By similarity
Binding site1451ATP By similarity
Binding site2171ATP By similarity
Binding site2441Substrate; via amide nitrogen By similarity

Sequences

Sequence LengthMass (Da)Tools
B7GQ20 [UniParc].

Last modified February 10, 2009. Version 1.
Checksum: 644F22DBCBC50A1D

FASTA31432,492
        10         20         30         40         50         60 
MSNSASSFAD VSSGCTAGTP VPADSPIRDN IADAVRRVRE TTPLAQSFTN FVTINLVANA 

        70         80         90        100        110        120 
QLAAGGTAAM SFLPDDVIET AKIAGANYIN VGTLLPFYKD ALPEIAQRLN YLDKPWVLDP 

       130        140        150        160        170        180 
VAAGIGHTRT AILQTFKAAP PTMIRANASE VIALANMWGL NTETVGDASE HRPAGVESVD 

       190        200        210        220        230        240 
DVESATGAAV ALAQYLTEQH AKHSSHDAST RCAVAVSGIA DLVTDGETVY RLPGGSAMMT 

       250        260        270        280        290        300 
KITGAGCSLG GVAATYLAVS DPLTAALSAS LLYNRAAEIA DTTSHGPGSF QVAFLDALWN 

       310 
VTAEQVAESE ILVQ 

« Hide

References

[1]"The genome sequence of Bifidobacterium longum subsp. infantis reveals adaptations for milk utilization within the infant microbiome."
Sela D.A., Chapman J., Adeuya A., Kim J.H., Chen F., Whitehead T.R., Lapidus A., Rokhsar D.S., Lebrilla C.B., German J.B., Price N.P., Richardson P.M., Mills D.A.
Proc. Natl. Acad. Sci. U.S.A. 105:18964-18969(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 15697 / DSM 20088 / JCM 1222 / NCTC 11817 / S12.
[2]"Bifidobacteria can protect from enteropathogenic infection through production of acetate."
Fukuda S., Toh H., Hase K., Oshima K., Nakanishi Y., Yoshimura K., Tobe T., Clarke J.M., Topping D.L., Suzuki T., Taylor T.D., Itoh K., Kikuchi J., Morita H., Hattori M., Ohno H.
Nature 469:543-547(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 15697 / DSM 20088 / JCM 1222 / NCTC 11817 / S12.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001095 Genomic DNA. Translation: ACJ51900.1.
AP010889 Genomic DNA. Translation: BAJ68403.1.
RefSeqYP_002322278.1. NC_011593.1.
YP_005584573.1. NC_017219.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING391904.Blon_0797.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACJ51900; ACJ51900; Blon_0797.
BAJ68403; BAJ68403; BLIJ_0811.
GeneID12166385.
7054828.
KEGGbln:Blon_0797.
blon:BLIJ_0811.
PATRIC21122540. VBIBifLon71229_0795.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG2145.
HOGENOMHOG000114352.
KOK00878.
OMASPVMAHA.
OrthoDBEOG628F8M.
ProtClustDBCLSK572499.

Enzyme and pathway databases

BioCycBLON391904:GCDR-819-MONOMER.
UniPathwayUPA00060; UER00139.

Family and domain databases

HAMAPMF_00228. Thz_kinase.
InterProIPR000417. Hyethyz_kinase.
[Graphical view]
PfamPF02110. HK. 1 hit.
[Graphical view]
PIRSFPIRSF000513. Thz_kinase. 1 hit.
PRINTSPR01099. HYETHTZKNASE.
ProtoNetSearch...

Entry information

Entry nameTHIM_BIFLS
AccessionPrimary (citable) accession number: B7GQ20
Secondary accession number(s): E8MQX8
Entry history
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: February 10, 2009
Last modified: February 19, 2014
This is version 34 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways