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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Bifidobacterium longum subsp. infantis (strain ATCC 15697 / DSM 20088 / JCM 1222 / NCTC 11817 / S12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathwayi: N(7)-methylguanine-tRNA biosynthesis

This protein is involved in the pathway N(7)-methylguanine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway N(7)-methylguanine-tRNA biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei91S-adenosyl-L-methionineUniRule annotation1
Binding sitei116S-adenosyl-L-methionineUniRule annotation1
Binding sitei143S-adenosyl-L-methionineUniRule annotation1
Active sitei165By similarity1
Binding sitei165S-adenosyl-L-methionineUniRule annotation1
Binding sitei169SubstrateUniRule annotation1
Binding sitei201SubstrateUniRule annotation1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:trmBUniRule annotation
Ordered Locus Names:Blon_0537, BLIJ_0540
OrganismiBifidobacterium longum subsp. infantis (strain ATCC 15697 / DSM 20088 / JCM 1222 / NCTC 11817 / S12)
Taxonomic identifieri391904 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaBifidobacterialesBifidobacteriaceaeBifidobacterium
Proteomesi
  • UP000001360 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001496461 – 286tRNA (guanine-N(7)-)-methyltransferaseAdd BLAST286

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni262 – 265Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000073968.
KOiK03439.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB. 1 hit.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B7GP38-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDPESTAID SVAAMATDHT EAPAHPLHKV LSFVRRSGRL DDRLQRAWDN
60 70 80 90 100
YAGTYLLDIA AGNLLDVREG VTLDRALVES AWGNDNPLIV EIGTGQGENV
110 120 130 140 150
VAAAAAHPET NFLALEVYDP GVAHTLLLAG KQGLTNIRVA QVNAPELFKV
160 170 180 190 200
TAPGTAAEVW TFFPDPWPKK KHHKRRIVQE AMAGDIHRAL AADGVWRIAT
210 220 230 240 250
DIEDYALHVH EVMDGLDGWK NLGSVTVSLP LEHVGKGNAD LAADMPHADF
260 270 280
TESERFEGRV LTNFEKKGLA AGRVIHDFTY QAVALH
Length:286
Mass (Da):31,140
Last modified:February 10, 2009 - v1
Checksum:i8ED56510E51E2CEE
GO

Sequence cautioni

The sequence BAJ68134 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001095 Genomic DNA. Translation: ACJ51650.1.
AP010889 Genomic DNA. Translation: BAJ68134.1. Different initiation.

Genome annotation databases

EnsemblBacteriaiACJ51650; ACJ51650; Blon_0537.
BAJ68134; BAJ68134; BLIJ_0540.
KEGGibln:Blon_0537.
blon:BLIJ_0540.
PATRICi21121984. VBIBifLon71229_0522.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001095 Genomic DNA. Translation: ACJ51650.1.
AP010889 Genomic DNA. Translation: BAJ68134.1. Different initiation.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACJ51650; ACJ51650; Blon_0537.
BAJ68134; BAJ68134; BLIJ_0540.
KEGGibln:Blon_0537.
blon:BLIJ_0540.
PATRICi21121984. VBIBifLon71229_0522.

Phylogenomic databases

HOGENOMiHOG000073968.
KOiK03439.

Enzyme and pathway databases

UniPathwayiUPA00989.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB. 1 hit.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTRMB_BIFLS
AccessioniPrimary (citable) accession number: B7GP38
Secondary accession number(s): E8MQ59
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: February 10, 2009
Last modified: November 30, 2016
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.