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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Bifidobacterium longum subsp. infantis (strain ATCC 15697 / DSM 20088 / JCM 1222 / NCTC 11817 / S12)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the formation of N7-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N7-methylguanine(46) in tRNA.UniRule annotation

Pathwayi: N(7)-methylguanine-tRNA biosynthesis

This protein is involved in the pathway N(7)-methylguanine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway N(7)-methylguanine-tRNA biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei91S-adenosyl-L-methionineUniRule annotation1
Binding sitei116S-adenosyl-L-methionineUniRule annotation1
Binding sitei143S-adenosyl-L-methionineUniRule annotation1
Active sitei165By similarity1
Binding sitei165S-adenosyl-L-methionineUniRule annotation1
Binding sitei169SubstrateUniRule annotation1
Binding sitei201SubstrateUniRule annotation1

GO - Molecular functioni

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processtRNA processing
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00989

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:trmBUniRule annotation
Ordered Locus Names:Blon_0537, BLIJ_0540
OrganismiBifidobacterium longum subsp. infantis (strain ATCC 15697 / DSM 20088 / JCM 1222 / NCTC 11817 / S12)
Taxonomic identifieri391904 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaBifidobacterialesBifidobacteriaceaeBifidobacterium
Proteomesi
  • UP000001360 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001496461 – 286tRNA (guanine-N(7)-)-methyltransferaseAdd BLAST286

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni262 – 265Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000073968
KOiK03439

Family and domain databases

HAMAPiMF_01057 tRNA_methyltr_TrmB, 1 hit
InterProiView protein in InterPro
IPR029063 SAM-dependent_MTases
IPR003358 tRNA_(Gua-N-7)_MeTrfase_Trmb
PANTHERiPTHR23417 PTHR23417, 1 hit
SUPFAMiSSF53335 SSF53335, 1 hit
TIGRFAMsiTIGR00091 TIGR00091, 1 hit
PROSITEiView protein in PROSITE
PS51625 SAM_MT_TRMB, 1 hit

Sequencei

Sequence statusi: Complete.

B7GP38-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDPESTAID SVAAMATDHT EAPAHPLHKV LSFVRRSGRL DDRLQRAWDN
60 70 80 90 100
YAGTYLLDIA AGNLLDVREG VTLDRALVES AWGNDNPLIV EIGTGQGENV
110 120 130 140 150
VAAAAAHPET NFLALEVYDP GVAHTLLLAG KQGLTNIRVA QVNAPELFKV
160 170 180 190 200
TAPGTAAEVW TFFPDPWPKK KHHKRRIVQE AMAGDIHRAL AADGVWRIAT
210 220 230 240 250
DIEDYALHVH EVMDGLDGWK NLGSVTVSLP LEHVGKGNAD LAADMPHADF
260 270 280
TESERFEGRV LTNFEKKGLA AGRVIHDFTY QAVALH
Length:286
Mass (Da):31,140
Last modified:February 10, 2009 - v1
Checksum:i8ED56510E51E2CEE
GO

Sequence cautioni

The sequence BAJ68134 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001095 Genomic DNA Translation: ACJ51650.1
AP010889 Genomic DNA Translation: BAJ68134.1 Different initiation.

Genome annotation databases

EnsemblBacteriaiACJ51650; ACJ51650; Blon_0537
BAJ68134; BAJ68134; BLIJ_0540
KEGGibln:Blon_0537
blon:BLIJ_0540
PATRICifig|391904.8.peg.540

Similar proteinsi

Entry informationi

Entry nameiTRMB_BIFLS
AccessioniPrimary (citable) accession number: B7GP38
Secondary accession number(s): E8MQ59
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: February 10, 2009
Last modified: April 25, 2018
This is version 56 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome
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Main funding by: National Institutes of Health