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B7GNK0 (PROB_BIFLS) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 42. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutamate 5-kinase

EC=2.7.2.11
Alternative name(s):
Gamma-glutamyl kinase
Short name=GK
Gene names
Name:proB
Ordered Locus Names:Blon_2298, BLIJ_2372
OrganismBifidobacterium longum subsp. infantis (strain ATCC 15697 / DSM 20088 / JCM 1222 / NCTC 11817 / S12) [Complete proteome] [HAMAP]
Taxonomic identifier391904 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeBifidobacterialesBifidobacteriaceaeBifidobacterium

Protein attributes

Sequence length377 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5-oxoproline By similarity. HAMAP-Rule MF_00456

Catalytic activity

ATP + L-glutamate = ADP + L-glutamate 5-phosphate. HAMAP-Rule MF_00456

Pathway

Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 1/2. HAMAP-Rule MF_00456

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00456.

Sequence similarities

Belongs to the glutamate 5-kinase family.

Contains 1 PUA domain.

Ontologies

Keywords
   Biological processAmino-acid biosynthesis
Proline biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionKinase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processL-proline biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

RNA binding

Inferred from electronic annotation. Source: InterPro

glutamate 5-kinase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 377377Glutamate 5-kinase HAMAP-Rule MF_00456
PRO_1000193688

Regions

Domain285 – 36379PUA
Nucleotide binding181 – 1822ATP By similarity
Nucleotide binding223 – 2297ATP By similarity

Sites

Binding site221ATP By similarity
Binding site621Substrate By similarity
Binding site1491Substrate By similarity
Binding site1611Substrate; via amide nitrogen By similarity

Sequences

Sequence LengthMass (Da)Tools
B7GNK0 [UniParc].

Last modified February 10, 2009. Version 1.
Checksum: 9AA9E115A5866A52

FASTA37739,216
        10         20         30         40         50         60 
MSTPSQAEVR RMVAAAGTIV VKVGSSSLTQ PSGHLDPDKL DALAAALAQI RLMGGRVVLV 

        70         80         90        100        110        120 
SSGAIAAGFG PLGFDERPAD VATQQATAAV GQGLLMARYE TAFGRFGIRV GQILITAEDT 

       130        140        150        160        170        180 
IRATQYRNVE RTLDRLLDLG VVPIINENDS LASNEIRFGD NDRLSALVAN LVRAEALVLL 

       190        200        210        220        230        240 
TDVDALYTAP PSQPGSRRVE YVPNVIDALG DIQVSGSGSK VGTGGMVTKL EAARVAAVSG 

       250        260        270        280        290        300 
IPTVLTCASN AGPAMMGDPV GTVFAPVKAR GSSRRLWIGF AADPRGAIVV DAGAGQAIRG 

       310        320        330        340        350        360 
GRASLLATGA LEVHGDFSAG DPVWIDAESG EHLARGLAGF DSEEIPQMLG RNTAQLKRFL 

       370 
GPQYAHPLIH RDNLVLV 

« Hide

References

[1]"The genome sequence of Bifidobacterium longum subsp. infantis reveals adaptations for milk utilization within the infant microbiome."
Sela D.A., Chapman J., Adeuya A., Kim J.H., Chen F., Whitehead T.R., Lapidus A., Rokhsar D.S., Lebrilla C.B., German J.B., Price N.P., Richardson P.M., Mills D.A.
Proc. Natl. Acad. Sci. U.S.A. 105:18964-18969(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 15697 / DSM 20088 / JCM 1222 / NCTC 11817 / S12.
[2]"Bifidobacteria can protect from enteropathogenic infection through production of acetate."
Fukuda S., Toh H., Hase K., Oshima K., Nakanishi Y., Yoshimura K., Tobe T., Clarke J.M., Topping D.L., Suzuki T., Taylor T.D., Itoh K., Kikuchi J., Morita H., Hattori M., Ohno H.
Nature 469:543-547(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 15697 / DSM 20088 / JCM 1222 / NCTC 11817 / S12.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001095 Genomic DNA. Translation: ACJ53356.1.
AP010889 Genomic DNA. Translation: BAJ69949.1.
RefSeqYP_002323734.1. NC_011593.1.
YP_005586119.1. NC_017219.1.

3D structure databases

ProteinModelPortalB7GNK0.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING391904.Blon_2298.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACJ53356; ACJ53356; Blon_2298.
BAJ69949; BAJ69949; BLIJ_2372.
GeneID12166692.
7054898.
KEGGbln:Blon_2298.
blon:BLIJ_2372.
PATRIC21125685. VBIBifLon71229_2308.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0263.
HOGENOMHOG000246368.
KOK00931.
OMAMRMIAGH.
OrthoDBEOG6PGK7G.

Enzyme and pathway databases

BioCycBLON391904:GCDR-2380-MONOMER.
UniPathwayUPA00098; UER00359.

Family and domain databases

Gene3D2.30.130.10. 1 hit.
3.40.1160.10. 1 hit.
HAMAPMF_00456. ProB.
InterProIPR001048. Asp/Glu/Uridylate_kinase.
IPR001057. Glu/AcGlu_kinase.
IPR011529. Glu_5kinase.
IPR005715. Glu_5kinase/COase_Synthase.
IPR019797. Glutamate_5-kinase_CS.
IPR002478. PUA.
IPR015947. PUA-like_domain.
[Graphical view]
PfamPF00696. AA_kinase. 1 hit.
PF01472. PUA. 1 hit.
[Graphical view]
PIRSFPIRSF000729. GK. 1 hit.
PRINTSPR00474. GLU5KINASE.
SMARTSM00359. PUA. 1 hit.
[Graphical view]
SUPFAMSSF53633. SSF53633. 1 hit.
SSF88697. SSF88697. 1 hit.
TIGRFAMsTIGR01027. proB. 1 hit.
PROSITEPS00902. GLUTAMATE_5_KINASE. 1 hit.
PS50890. PUA. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePROB_BIFLS
AccessionPrimary (citable) accession number: B7GNK0
Secondary accession number(s): E8MNT3
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: February 10, 2009
Last modified: May 14, 2014
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways