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Protein

Polyribonucleotide nucleotidyltransferase

Gene

pnp

Organism
Bifidobacterium longum subsp. infantis (strain ATCC 15697 / DSM 20088 / JCM 1222 / NCTC 11817 / S12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction.UniRule annotation

Catalytic activityi

RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi521 – 5211MagnesiumUniRule annotation
Metal bindingi527 – 5271MagnesiumUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding, RNA-binding

Enzyme and pathway databases

BioCyciBLON391904:GCDR-2347-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Polyribonucleotide nucleotidyltransferaseUniRule annotation (EC:2.7.7.8UniRule annotation)
Alternative name(s):
Polynucleotide phosphorylaseUniRule annotation
Short name:
PNPaseUniRule annotation
Gene namesi
Name:pnpUniRule annotation
Ordered Locus Names:Blon_2270, BLIJ_2342
OrganismiBifidobacterium longum subsp. infantis (strain ATCC 15697 / DSM 20088 / JCM 1222 / NCTC 11817 / S12)
Taxonomic identifieri391904 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaBifidobacterialesBifidobacteriaceaeBifidobacterium
ProteomesiUP000001360 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 914914Polyribonucleotide nucleotidyltransferasePRO_0000381867Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliB7GNH2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini587 – 64660KHUniRule annotationAdd
BLAST
Domaini658 – 73073S1 motifUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the polyribonucleotide nucleotidyltransferase family.UniRule annotation
Contains 1 KH domain.UniRule annotation
Contains 1 S1 motif domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG1185.
HOGENOMiHOG000218326.
KOiK00962.
OMAiRFMFHYN.
OrthoDBiEOG6WT8CC.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR014069. pppGpp_PNP.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 1 hit.
PF00575. S1. 1 hit.
[Graphical view]
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
TIGR02696. pppGpp_PNP. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B7GNH2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEGPEIKAVE AVIDNGSFGK RTLRFETGRL AQQADGAVAA YLDDDSMILS
60 70 80 90 100
TTTAGSSPKE NYDFFPLTVD VEEKMYAAGK IPGSFFRREG RPSSEAILAC
110 120 130 140 150
RIIDRPLRPL FPHTLRNEVQ VVETVLAVNP DDAYDVIALN AASASTMISG
160 170 180 190 200
LPFEGPVSGV RLALIDGQWV AFPRWSERER AVFEIVVAGR VVENGDVAIA
210 220 230 240 250
MIEAGAGKNA WHLIYDEGQT KPDEEVVAGG LEAAKPFIKV ICEAQDELKK
260 270 280 290 300
IAAKETKEFQ LFPEYTDELY ARIDEIAHKD LDEALSIAEK LPRQDRIHEI
310 320 330 340 350
KEHVREVLAD EFTDMDDAEK DKELGNAFKE LQRQIVRRRI LTEDYRIDGR
360 370 380 390 400
GLRDIRTLSA EVDIVPRVHG SALFQRGETQ ILGVTTLNML KMEQQIDALS
410 420 430 440 450
GPQSKRYMHN YEMPPYSTGE TGRVGSPKRR EIGHGALAEK ALVPVLPSRE
460 470 480 490 500
EFPYAIRQVS EAIGSNGSTS MGSVCASTLS LLAAGVPLKA PVAGIAMGLV
510 520 530 540 550
SGDVDGQHIF KTLTDILGAE DAFGDMDFKV AGTSEFITAL QLDTKLDGIP
560 570 580 590 600
ADILAAALQQ AKEARATILE VINECIDGPA EMSEFAPRII TTSVPVEKIG
610 620 630 640 650
EVIGPKGKMI NQIQEDTGAE IAIEDDGTVF ISSEGGEAAK KAKSIIDSIA
660 670 680 690 700
NPHVPEAGET YNGKVVKTTS FGAFVNLTPG TDGLLHISQI RNLANGERID
710 720 730 740 750
AVEDVLREGD TVEVIVQGVD DRGKISLAIP GFEDQENNAR PSRGDRDDRR
760 770 780 790 800
GGRGRGDRDD RRGGRGRRSD RDDRDFDDRD DRPRRRRSDD FEDDYDDRPR
810 820 830 840 850
RRRSDDRDFD RDDRDDDRPR RRRSADRDFD DRDDRDARDS RDDDRPRRRR
860 870 880 890 900
SADRDDRGDR DDRRGGFRGG RGRGSDRNPR YATDDNYDDY RADREERTER
910
PRRRVRRDFD PFED
Length:914
Mass (Da):101,922
Last modified:February 10, 2009 - v1
Checksum:i24F9B6F629FCA9E1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001095 Genomic DNA. Translation: ACJ53328.1.
AP010889 Genomic DNA. Translation: BAJ69919.1.
RefSeqiWP_012578501.1. NC_017219.1.

Genome annotation databases

EnsemblBacteriaiACJ53328; ACJ53328; Blon_2270.
BAJ69919; BAJ69919; BLIJ_2342.
KEGGibln:Blon_2270.
blon:BLIJ_2342.
PATRICi21125619. VBIBifLon71229_2280.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001095 Genomic DNA. Translation: ACJ53328.1.
AP010889 Genomic DNA. Translation: BAJ69919.1.
RefSeqiWP_012578501.1. NC_017219.1.

3D structure databases

ProteinModelPortaliB7GNH2.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACJ53328; ACJ53328; Blon_2270.
BAJ69919; BAJ69919; BLIJ_2342.
KEGGibln:Blon_2270.
blon:BLIJ_2342.
PATRICi21125619. VBIBifLon71229_2280.

Phylogenomic databases

eggNOGiCOG1185.
HOGENOMiHOG000218326.
KOiK00962.
OMAiRFMFHYN.
OrthoDBiEOG6WT8CC.

Enzyme and pathway databases

BioCyciBLON391904:GCDR-2347-MONOMER.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR014069. pppGpp_PNP.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 1 hit.
PF00575. S1. 1 hit.
[Graphical view]
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
TIGR02696. pppGpp_PNP. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The genome sequence of Bifidobacterium longum subsp. infantis reveals adaptations for milk utilization within the infant microbiome."
    Sela D.A., Chapman J., Adeuya A., Kim J.H., Chen F., Whitehead T.R., Lapidus A., Rokhsar D.S., Lebrilla C.B., German J.B., Price N.P., Richardson P.M., Mills D.A.
    Proc. Natl. Acad. Sci. U.S.A. 105:18964-18969(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 15697 / DSM 20088 / JCM 1222 / NCTC 11817 / S12.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 15697 / DSM 20088 / JCM 1222 / NCTC 11817 / S12.

Entry informationi

Entry nameiPNP_BIFLS
AccessioniPrimary (citable) accession number: B7GNH2
Secondary accession number(s): E8MNQ3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 1, 2009
Last sequence update: February 10, 2009
Last modified: July 22, 2015
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.