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Protein

dITP/XTP pyrophosphatase

Gene

Blon_0412

Organism
Bifidobacterium longum subsp. infantis (strain ATCC 15697 / DSM 20088 / JCM 1222 / NCTC 11817 / S12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Pyrophosphatase that catalyzes the hydrolysis of nucleoside triphosphates to their monophosphate derivatives, with a high preference for the non-canonical purine nucleotides XTP (xanthosine triphosphate), dITP (deoxyinosine triphosphate) and ITP. Seems to function as a house-cleaning enzyme that removes non-canonical purine nucleotides from the nucleotide pool, thus preventing their incorporation into DNA/RNA and avoiding chromosomal lesions.UniRule annotation

Catalytic activityi

XTP + H2O = XMP + diphosphate.UniRule annotation
dITP + H2O = dIMP + diphosphate.UniRule annotation
ITP + H2O = IMP + diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei74Proton acceptorUniRule annotation1
Metal bindingi74MagnesiumUniRule annotation1
Binding sitei75Substrate; via amide nitrogenUniRule annotation1
Binding sitei230SubstrateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processNucleotide metabolism
LigandMagnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
dITP/XTP pyrophosphataseUniRule annotation (EC:3.6.1.66UniRule annotation)
Alternative name(s):
Non-canonical purine NTP pyrophosphataseUniRule annotation
Non-standard purine NTP pyrophosphataseUniRule annotation
Nucleoside-triphosphate diphosphataseUniRule annotation
Nucleoside-triphosphate pyrophosphataseUniRule annotation
Short name:
NTPaseUniRule annotation
Gene namesi
Ordered Locus Names:Blon_0412, BLIJ_0420
OrganismiBifidobacterium longum subsp. infantis (strain ATCC 15697 / DSM 20088 / JCM 1222 / NCTC 11817 / S12)
Taxonomic identifieri391904 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaBifidobacterialesBifidobacteriaceaeBifidobacterium
Proteomesi
  • UP000001360 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001845781 – 252dITP/XTP pyrophosphataseAdd BLAST252

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni7 – 12Substrate bindingUniRule annotation6
Regioni193 – 196Substrate bindingUniRule annotation4
Regioni235 – 236Substrate bindingUniRule annotation2

Sequence similaritiesi

Belongs to the HAM1 NTPase family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000293319.
KOiK02428.
OMAiTHNPGKV.

Family and domain databases

CDDicd00515. HAM1. 1 hit.
Gene3Di3.90.950.10. 1 hit.
HAMAPiMF_01405. Non_canon_purine_NTPase. 1 hit.
InterProiView protein in InterPro
IPR002637. Ham1p-like.
IPR029001. ITPase-like_fam.
IPR020922. NTPase.
PANTHERiPTHR11067. PTHR11067. 1 hit.
PfamiView protein in Pfam
PF01725. Ham1p_like. 1 hit.
SUPFAMiSSF52972. SSF52972. 2 hits.

Sequencei

Sequence statusi: Complete.

B7GN27-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQIVVATHNE GKLVEIRRIL EEDLGVDAEN IELVSAGSLH LPDPVETGVT
60 70 80 90 100
FQENALLKAR AVAIRTGLPA VADDSGLIVD VMGNAPGILS ARWAGAHGHD
110 120 130 140 150
KANNALLLAQ IGDIPDDKRT ARFRCAAALV VPDTEAGADV TGGVAADGIT
160 170 180 190 200
VHTTAADGST APVHARYAIK SETVELGDMP GRIIREARGE HGFGYDPLFV
210 220 230 240 250
PDDQPAGRVS TEPDHEGEPL TSAEMTSAEK NAISHRGKAL KALVPAIEAL

LH
Length:252
Mass (Da):26,368
Last modified:February 10, 2009 - v1
Checksum:i0C3290BF5D6A6DD4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001095 Genomic DNA. Translation: ACJ51533.1.
AP010889 Genomic DNA. Translation: BAJ68014.1.
RefSeqiWP_012576833.1. NZ_JDTT01000012.1.

Genome annotation databases

EnsemblBacteriaiACJ51533; ACJ51533; Blon_0412.
BAJ68014; BAJ68014; BLIJ_0420.
KEGGibln:Blon_0412.
blon:BLIJ_0420.
PATRICi21121758. VBIBifLon71229_0413.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001095 Genomic DNA. Translation: ACJ51533.1.
AP010889 Genomic DNA. Translation: BAJ68014.1.
RefSeqiWP_012576833.1. NZ_JDTT01000012.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACJ51533; ACJ51533; Blon_0412.
BAJ68014; BAJ68014; BLIJ_0420.
KEGGibln:Blon_0412.
blon:BLIJ_0420.
PATRICi21121758. VBIBifLon71229_0413.

Phylogenomic databases

HOGENOMiHOG000293319.
KOiK02428.
OMAiTHNPGKV.

Family and domain databases

CDDicd00515. HAM1. 1 hit.
Gene3Di3.90.950.10. 1 hit.
HAMAPiMF_01405. Non_canon_purine_NTPase. 1 hit.
InterProiView protein in InterPro
IPR002637. Ham1p-like.
IPR029001. ITPase-like_fam.
IPR020922. NTPase.
PANTHERiPTHR11067. PTHR11067. 1 hit.
PfamiView protein in Pfam
PF01725. Ham1p_like. 1 hit.
SUPFAMiSSF52972. SSF52972. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiIXTPA_BIFLS
AccessioniPrimary (citable) accession number: B7GN27
Secondary accession number(s): E8MPT9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: February 10, 2009
Last modified: April 12, 2017
This is version 50 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.