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Protein

Non-canonical purine NTP pyrophosphatase

Gene

Blon_0412

Organism
Bifidobacterium longum subsp. infantis (strain ATCC 15697 / DSM 20088 / JCM 1222 / NCTC 11817 / S12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Pyrophosphatase that hydrolyzes non-canonical purine nucleotides such as XTP and ITP/dITP to their respective monophosphate derivatives. Might exclude non-canonical purines from DNA precursor pool, thus preventing their incorporation into DNA and avoiding chromosomal lesions.UniRule annotation

Catalytic activityi

A nucleoside triphosphate + H2O = a nucleotide + diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation, Mn2+UniRule annotationNote: Binds 1 divalent metal cation per subunit; can use either Mg(2+) or Mn2+.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi74 – 741Magnesium or manganeseUniRule annotation
Binding sitei196 – 1961SubstrateUniRule annotation
Binding sitei230 – 2301SubstrateUniRule annotation
Binding sitei236 – 2361SubstrateUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Nucleotide metabolism

Keywords - Ligandi

Magnesium, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBLON391904:GCDR-425-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Non-canonical purine NTP pyrophosphataseUniRule annotation (EC:3.6.1.19UniRule annotation)
Alternative name(s):
Non-standard purine NTP pyrophosphataseUniRule annotation
Nucleoside-triphosphate diphosphataseUniRule annotation
Nucleoside-triphosphate pyrophosphataseUniRule annotation
Short name:
NTPaseUniRule annotation
Gene namesi
Ordered Locus Names:Blon_0412, BLIJ_0420
OrganismiBifidobacterium longum subsp. infantis (strain ATCC 15697 / DSM 20088 / JCM 1222 / NCTC 11817 / S12)
Taxonomic identifieri391904 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaBifidobacterialesBifidobacteriaceaeBifidobacterium
ProteomesiUP000001360 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 252252Non-canonical purine NTP pyrophosphatasePRO_1000184578Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni7 – 126Substrate bindingUniRule annotation
Regioni74 – 752Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the HAM1 NTPase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0127.
HOGENOMiHOG000293319.
KOiK02428.
OMAiYDPILQP.
OrthoDBiEOG6CZQQP.

Family and domain databases

Gene3Di3.90.950.10. 2 hits.
HAMAPiMF_01405. Non_canon_purine_NTPase.
InterProiIPR002637. Ham1p-like.
IPR029001. ITPase-like_fam.
IPR020922. NTPase.
[Graphical view]
PANTHERiPTHR11067. PTHR11067. 1 hit.
PfamiPF01725. Ham1p_like. 1 hit.
[Graphical view]
SUPFAMiSSF52972. SSF52972. 2 hits.

Sequencei

Sequence statusi: Complete.

B7GN27-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQIVVATHNE GKLVEIRRIL EEDLGVDAEN IELVSAGSLH LPDPVETGVT
60 70 80 90 100
FQENALLKAR AVAIRTGLPA VADDSGLIVD VMGNAPGILS ARWAGAHGHD
110 120 130 140 150
KANNALLLAQ IGDIPDDKRT ARFRCAAALV VPDTEAGADV TGGVAADGIT
160 170 180 190 200
VHTTAADGST APVHARYAIK SETVELGDMP GRIIREARGE HGFGYDPLFV
210 220 230 240 250
PDDQPAGRVS TEPDHEGEPL TSAEMTSAEK NAISHRGKAL KALVPAIEAL

LH
Length:252
Mass (Da):26,368
Last modified:February 10, 2009 - v1
Checksum:i0C3290BF5D6A6DD4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001095 Genomic DNA. Translation: ACJ51533.1.
AP010889 Genomic DNA. Translation: BAJ68014.1.
RefSeqiWP_012576833.1. NZ_JDTT01000012.1.

Genome annotation databases

EnsemblBacteriaiACJ51533; ACJ51533; Blon_0412.
BAJ68014; BAJ68014; BLIJ_0420.
KEGGibln:Blon_0412.
blon:BLIJ_0420.
PATRICi21121758. VBIBifLon71229_0413.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001095 Genomic DNA. Translation: ACJ51533.1.
AP010889 Genomic DNA. Translation: BAJ68014.1.
RefSeqiWP_012576833.1. NZ_JDTT01000012.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACJ51533; ACJ51533; Blon_0412.
BAJ68014; BAJ68014; BLIJ_0420.
KEGGibln:Blon_0412.
blon:BLIJ_0420.
PATRICi21121758. VBIBifLon71229_0413.

Phylogenomic databases

eggNOGiCOG0127.
HOGENOMiHOG000293319.
KOiK02428.
OMAiYDPILQP.
OrthoDBiEOG6CZQQP.

Enzyme and pathway databases

BioCyciBLON391904:GCDR-425-MONOMER.

Family and domain databases

Gene3Di3.90.950.10. 2 hits.
HAMAPiMF_01405. Non_canon_purine_NTPase.
InterProiIPR002637. Ham1p-like.
IPR029001. ITPase-like_fam.
IPR020922. NTPase.
[Graphical view]
PANTHERiPTHR11067. PTHR11067. 1 hit.
PfamiPF01725. Ham1p_like. 1 hit.
[Graphical view]
SUPFAMiSSF52972. SSF52972. 2 hits.
ProtoNetiSearch...

Publicationsi

  1. "The genome sequence of Bifidobacterium longum subsp. infantis reveals adaptations for milk utilization within the infant microbiome."
    Sela D.A., Chapman J., Adeuya A., Kim J.H., Chen F., Whitehead T.R., Lapidus A., Rokhsar D.S., Lebrilla C.B., German J.B., Price N.P., Richardson P.M., Mills D.A.
    Proc. Natl. Acad. Sci. U.S.A. 105:18964-18969(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 15697 / DSM 20088 / JCM 1222 / NCTC 11817 / S12.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 15697 / DSM 20088 / JCM 1222 / NCTC 11817 / S12.

Entry informationi

Entry nameiNTPA_BIFLS
AccessioniPrimary (citable) accession number: B7GN27
Secondary accession number(s): E8MPT9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: February 10, 2009
Last modified: July 22, 2015
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.