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B7GN27 (NTPA_BIFLS) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 35. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Non-canonical purine NTP pyrophosphatase

EC=3.6.1.19
Alternative name(s):
Non-standard purine NTP pyrophosphatase
Nucleoside-triphosphate diphosphatase
Nucleoside-triphosphate pyrophosphatase
Short name=NTPase
Gene names
Ordered Locus Names:Blon_0412, BLIJ_0420
OrganismBifidobacterium longum subsp. infantis (strain ATCC 15697 / DSM 20088 / JCM 1222 / NCTC 11817 / S12) [Complete proteome] [HAMAP]
Taxonomic identifier391904 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeBifidobacterialesBifidobacteriaceaeBifidobacterium

Protein attributes

Sequence length252 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Pyrophosphatase that hydrolyzes non-canonical purine nucleotides such as XTP and ITP/dITP to their respective monophosphate derivatives. Might exclude non-canonical purines from DNA precursor pool, thus preventing their incorporation into DNA and avoiding chromosomal lesions By similarity. HAMAP-Rule MF_01405

Catalytic activity

A nucleoside triphosphate + H2O = a nucleotide + diphosphate. HAMAP-Rule MF_01405

Cofactor

Binds 1 divalent metal cation ion per subunit; can use either magnesium or manganese By similarity.

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01405

Sequence similarities

Belongs to the HAM1 NTPase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 252252Non-canonical purine NTP pyrophosphatase HAMAP-Rule MF_01405
PRO_1000184578

Regions

Region7 – 126Substrate binding By similarity
Region74 – 752Substrate binding By similarity

Sites

Metal binding741Magnesium or manganese By similarity
Binding site1961Substrate By similarity
Binding site2301Substrate By similarity
Binding site2361Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
B7GN27 [UniParc].

Last modified February 10, 2009. Version 1.
Checksum: 0C3290BF5D6A6DD4

FASTA25226,368
        10         20         30         40         50         60 
MQIVVATHNE GKLVEIRRIL EEDLGVDAEN IELVSAGSLH LPDPVETGVT FQENALLKAR 

        70         80         90        100        110        120 
AVAIRTGLPA VADDSGLIVD VMGNAPGILS ARWAGAHGHD KANNALLLAQ IGDIPDDKRT 

       130        140        150        160        170        180 
ARFRCAAALV VPDTEAGADV TGGVAADGIT VHTTAADGST APVHARYAIK SETVELGDMP 

       190        200        210        220        230        240 
GRIIREARGE HGFGYDPLFV PDDQPAGRVS TEPDHEGEPL TSAEMTSAEK NAISHRGKAL 

       250 
KALVPAIEAL LH 

« Hide

References

[1]"The genome sequence of Bifidobacterium longum subsp. infantis reveals adaptations for milk utilization within the infant microbiome."
Sela D.A., Chapman J., Adeuya A., Kim J.H., Chen F., Whitehead T.R., Lapidus A., Rokhsar D.S., Lebrilla C.B., German J.B., Price N.P., Richardson P.M., Mills D.A.
Proc. Natl. Acad. Sci. U.S.A. 105:18964-18969(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 15697 / DSM 20088 / JCM 1222 / NCTC 11817 / S12.
[2]"Bifidobacteria can protect from enteropathogenic infection through production of acetate."
Fukuda S., Toh H., Hase K., Oshima K., Nakanishi Y., Yoshimura K., Tobe T., Clarke J.M., Topping D.L., Suzuki T., Taylor T.D., Itoh K., Kikuchi J., Morita H., Hattori M., Ohno H.
Nature 469:543-547(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 15697 / DSM 20088 / JCM 1222 / NCTC 11817 / S12.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001095 Genomic DNA. Translation: ACJ51533.1.
AP010889 Genomic DNA. Translation: BAJ68014.1.
RefSeqYP_002321911.1. NC_011593.1.
YP_005584184.1. NC_017219.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING391904.Blon_0412.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACJ51533; ACJ51533; Blon_0412.
BAJ68014; BAJ68014; BLIJ_0420.
GeneID12167331.
7053505.
KEGGbln:Blon_0412.
blon:BLIJ_0420.
PATRIC21121758. VBIBifLon71229_0413.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0127.
HOGENOMHOG000293319.
KOK02428.
OMAEAIIATH.
OrthoDBEOG6CZQQP.

Enzyme and pathway databases

BioCycBLON391904:GCDR-425-MONOMER.

Family and domain databases

Gene3D3.90.950.10. 2 hits.
HAMAPMF_01405. Non_canon_purine_NTPase.
InterProIPR002637. Ham1p-like.
IPR029001. ITPase-like_fam.
IPR020922. NTPase.
[Graphical view]
PANTHERPTHR11067. PTHR11067. 1 hit.
PfamPF01725. Ham1p_like. 1 hit.
[Graphical view]
SUPFAMSSF52972. SSF52972. 2 hits.
ProtoNetSearch...

Entry information

Entry nameNTPA_BIFLS
AccessionPrimary (citable) accession number: B7GN27
Secondary accession number(s): E8MPT9
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: February 10, 2009
Last modified: June 11, 2014
This is version 35 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families