Reviewed, UniProtKB/Swiss-Prot B7GMJ3 (SYR_ANOFW)

Names and origin

Protein names

Recommended name:
    Arginyl-tRNA synthetase
    EC=6.1.1.19
Alternative name(s):
    Arginine--tRNA ligase
      Short name=ArgRS

Gene names

Name:	argS
Ordered Locus Names:	Aflv_2742

Organism

Anoxybacillus flavithermus (strain DSM 21510 / WK1) [Complete proteome] [HAMAP]

Taxonomic identifier

491915 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Anoxybacillus

Protein attributes

Sequence length	556 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-arginine + tRNA(Arg) = AMP + diphosphate + L-arginyl-tRNA(Arg). HAMAP MF_00123
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP arginine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 556	556	Arginyl-tRNA synthetase HAMAP MF_00123		PRO_1000198871
Regions
	Motif	132 – 142	11	"HIGH" region HAMAP MF_00123

Sequences

	Sequence		Length	Mass (Da)
	B7GMJ3-1 [UniParc]. Last modified February 10, 2009. Version 1. Checksum: 28CD7D5B34821F5E	FASTA	556	62,636
	10 20 30 40 50 60 MNIVEQMKQK LKEEIKEAVI RSGVATEADV PSVILETPKD KAHGDYSTNV AMQLARVAKK 70 80 90 100 110 120 APRMIAEDIV RHFNGEAVFV KKVDIAGPGF INFYMDNRYL TELVPAILQA GDAYGETNVG 130 140 150 160 170 180 QGQKVQVEFV SANPTGSLHL GHARGAAVGD SLCNILKKAG FDVTREYYIN DAGNQIANLA 190 200 210 220 230 240 KSVEARYFQA LGIKKDMPED GYFGEDIIEL GKKLAEEHGD KFVALDEQER LQLFREYGLA 250 260 270 280 290 300 FEMDKIKKDL ADFRVTFDVW YSETSLYHNG KIDKALETLR EKGHIYEQDG ATWFRSTTFG 310 320 330 340 350 360 DDKDRVLIKQ DGTYTYLLPD IAYHQDKLER GFEKIINIWG ADHHGYIPRM KAAIAALGYD 370 380 390 400 410 420 PDVLEVEIIQ LVSLYQNGEK VRMSKRTGKA VTLRDLMEEV GLDATRYFFA MRSSDTHLDF 430 440 450 460 470 480 DMDLAVSQSN ENPVYYAQYA HARVCSMLRQ GEEQGWRYDG DLSFTYELAE KEIDLLKKLG 490 500 510 520 530 540 EFPSAVAEAA VKRSPHRMTN YIFDLASALH SFYNAEKVLD AANVEKSRAR LALMKAVQIT 550 LKNALALVGV HAPEKM

References

[1]

"Encapsulated in silica: genome, proteome and physiology of the thermophilic bacterium Anoxybacillus flavithermus WK1." Saw J.H., Mountain B.W., Feng L., Omelchenko M.V., Hou S., Saito J.A., Stott M.B., Li D., Zhao G., Wu J., Galperin M.Y., Koonin E.V., Makarova K.S., Wolf Y.I., Rigden D.J., Dunfield P.F., Wang L., Alam M. Genome Biol. 9:RESEARCH161.1-RESEARCH161.16(2008) [PubMed: 19014707] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000922 Genomic DNA. Translation: ACJ35095.1.
RefSeq	YP_002317080.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	7039015.
GenomeReviews	Gene locus Aflv_2742 in contig CP000922_GR.
KEGG	afl:Aflv_2742.
Organism-specific databases
CMR	Search...
Family and domain databases
HAMAP	MF_00123. [Tree]
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR001278. Arg-tRNA-synth_Ic. IPR015945. Arg-tRNA-synth_Ic_core. IPR005148. Arg-tRNA-synth_Ic_N. IPR008909. DALR_anticod_bd. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.30.1360.70. Arg-tRNA-synth_Ic_N. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHER	PTHR11956. Arg_tRNA-synt_1c. 1 hit.
Pfam	PF03485. Arg_tRNA_synt_N. 1 hit. PF05746. DALR_1. 1 hit. PF00750. tRNA-synt_1d. 1 hit. [Graphical view]
PRINTS	PR01038. TRNASYNTHARG.
TIGRFAMs	TIGR00456. argS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYR_ANOFW

Accession

Primary (citable) accession number: B7GMJ3

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	February 10, 2009
Last modified:	July 28, 2009
This is version 7 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)