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B7GME7 (NUOH_ANOFW) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 35. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
NADH-quinone oxidoreductase subunit H

EC=1.6.99.5
Alternative name(s):
NADH dehydrogenase I subunit H
NDH-1 subunit H
Gene names
Name:nuoH
Ordered Locus Names:Aflv_2696
OrganismAnoxybacillus flavithermus (strain DSM 21510 / WK1) [Complete proteome] [HAMAP]
Taxonomic identifier491915 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeAnoxybacillus

Protein attributes

Sequence length333 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient. This subunit may bind ubiquinone By similarity. HAMAP-Rule MF_01350

Catalytic activity

NADH + quinone = NAD+ + quinol. HAMAP-Rule MF_01350

Subunit structure

NDH-1 is composed of 14 different subunits. Subunits NuoA, H, J, K, L, M, N constitute the membrane sector of the complex By similarity.

Subcellular location

Cell membrane; Multi-pass membrane protein Potential HAMAP-Rule MF_01350.

Sequence similarities

Belongs to the complex I subunit 1 family.

Ontologies

Keywords
   Cellular componentCell membrane
Membrane
   DomainTransmembrane
Transmembrane helix
   LigandNAD
Ubiquinone
   Molecular functionOxidoreductase
   PTMQuinone
   Technical termComplete proteome
Gene Ontology (GO)
   Cellular_componentintegral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

plasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionNADH dehydrogenase (quinone) activity

Inferred from electronic annotation. Source: UniProtKB-EC

quinone binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 333333NADH-quinone oxidoreductase subunit H HAMAP-Rule MF_01350
PRO_1000143570

Regions

Transmembrane17 – 3721Helical; Potential
Transmembrane88 – 10821Helical; Potential
Transmembrane117 – 13721Helical; Potential
Transmembrane159 – 17921Helical; Potential
Transmembrane191 – 21121Helical; Potential
Transmembrane241 – 26121Helical; Potential
Transmembrane273 – 29321Helical; Potential
Transmembrane313 – 33321Helical; Potential

Sequences

Sequence LengthMass (Da)Tools
B7GME7 [UniParc].

Last modified February 10, 2009. Version 1.
Checksum: 90A3150FBBF2083F

FASTA33336,664
        10         20         30         40         50         60 
MMEQLLQSTP SWTNVAIFFA IGVGLLLVVL GFVTYGILAE RKVMGFMQGR IGPNQVGGRW 

        70         80         90        100        110        120 
GLLQTVADVL KLLLKEDTIP KAADRPLFIL APVIAFAPAF MVLAALPFTD ALQFADIGVG 

       130        140        150        160        170        180 
LLYYIAVSGL TTIGVVTGAW ASNNKYALLG GMRAAAQMIS YEVPLVMSVI GVILLSGSLN 

       190        200        210        220        230        240 
LNDIVAAQKD VWFIVVQPIG FLVFLIAAVA ELNRTPFDLP EAESELVAGF HVEYSGFRWA 

       250        260        270        280        290        300 
FFMLAEYVYF FAMAALTTVL FLGGWHPLPF LGFIPGAVWF ALKFSLVVFL FIWFRITFPR 

       310        320        330 
VRADQLMEFG WKVLLPVALV NIFVTALVKQ LFF 

« Hide

References

[1]"Encapsulated in silica: genome, proteome and physiology of the thermophilic bacterium Anoxybacillus flavithermus WK1."
Saw J.H., Mountain B.W., Feng L., Omelchenko M.V., Hou S., Saito J.A., Stott M.B., Li D., Zhao G., Wu J., Galperin M.Y., Koonin E.V., Makarova K.S., Wolf Y.I., Rigden D.J., Dunfield P.F., Wang L., Alam M.
Genome Biol. 9:R161.1-R161.16(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 21510 / WK1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000922 Genomic DNA. Translation: ACJ35049.1.
RefSeqYP_002317034.1. NC_011567.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING491915.Aflv_2696.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACJ35049; ACJ35049; Aflv_2696.
GeneID7038969.
KEGGafl:Aflv_2696.
PATRIC20957388. VBIAnoFla45531_2779.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1005.
HOGENOMHOG000228276.
KOK00337.
OMAGAFRNFA.
OrthoDBEOG6B62B4.

Enzyme and pathway databases

BioCycAFLA491915:GHEO-2785-MONOMER.

Family and domain databases

HAMAPMF_01350. NDH1_NuoH.
InterProIPR001694. NADH_UbQ_OxRdtase_su1/FPO.
IPR018086. NADH_UbQ_OxRdtase_su1_CS.
[Graphical view]
PANTHERPTHR11432. PTHR11432. 1 hit.
PfamPF00146. NADHdh. 1 hit.
[Graphical view]
PROSITEPS00668. COMPLEX1_ND1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNUOH_ANOFW
AccessionPrimary (citable) accession number: B7GME7
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: February 10, 2009
Last modified: May 14, 2014
This is version 35 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families