B7GLK2 (PRPE_ANOFW) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Bis(5'-nucleosyl)-tetraphosphatase prpE [asymmetrical]
EC=3.6.1.17

Alternative name(s):
Ap4A hydrolase
Diadenosine 5',5'''-P1,P4-tetraphosphate asymmetrical hydrolase
Short name=Diadenosine tetraphosphatase

Gene names

Name:	prpE
Ordered Locus Names:	Aflv_2112

Organism

Anoxybacillus flavithermus (strain DSM 21510 / WK1) [Complete proteome] [HAMAP]

Taxonomic identifier

491915 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Anoxybacillus

Protein attributes

Sequence length	245 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Asymmetrically hydrolyzes Ap4p to yield AMP and ATP By similarity. HAMAP MF_01443
Catalytic activity	P1,P(4)-bis(5'-guanosyl) tetraphosphate + H2O = GTP + GMP. HAMAP MF_01443
Cofactor	Nickel By similarity. HAMAP MF_01443
Sequence similarities	Belongs to the prpE family.

Ontologies

Keywords
Ligand	Nickel
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	bis(5'-nucleosyl)-tetraphosphatase (asymmetrical) activity Inferred from electronic annotation. Source: EC nickel cation binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 245	245	Bis(5'-nucleosyl)-tetraphosphatase prpE [asymmetrical] HAMAP MF_01443		PRO_1000145926

Sequences

	Sequence		Length	Mass (Da)
	B7GLK2 [UniParc]. Last modified February 10, 2009. Version 1. Checksum: 3FFE2F79986D4F48	FASTA	245	27,854
	10 20 30 40 50 60 MIYDIIGDIH GCFHEFMLLT KKLGYVWEND VPIHPNGRKL AFVGDLADRG PQSLQVIDTV 70 80 90 100 110 120 ISLVQQNKAV YVPGNHCDKL YRFFLGRNVQ VTHGLETTVA EWEQADEKTK TRIRTQFMKL 130 140 150 160 170 180 YENAPLYAIL DDGKLVIAHA GIRGDYIGKS NQKVKKFVLY GDITGEKHPD GSPVRRDWAK 190 200 210 220 230 240 HYRGDALIVY GHTPVKEPRW LNNTVNIDTG CVFGGQLTAL RYPEMETVSV PSTMPYVAEK FRPFD

References

[1]

"Encapsulated in silica: genome, proteome and physiology of the thermophilic bacterium Anoxybacillus flavithermus WK1." Saw J.H., Mountain B.W., Feng L., Omelchenko M.V., Hou S., Saito J.A., Stott M.B., Li D., Zhao G., Wu J., Galperin M.Y., Koonin E.V., Makarova K.S., Wolf Y.I., Rigden D.J., Dunfield P.F., Wang L., Alam M. Genome Biol. 9:R161.1-R161.16(2008) [PubMed: 19014707] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 21510 / WK1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000922 Genomic DNA. Translation: ACJ34471.1.
RefSeq	YP_002316456.1. NC_011567.1.
3D structure databases
ProteinModelPortal	B7GLK2.
ModBase	Search...
Protein-protein interaction databases
STRING	B7GLK2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7038365.
GenomeReviews	Gene locus Aflv_2112 in contig CP000922_GR.
KEGG	afl:Aflv_2112.
PATRIC	20956123. VBIAnoFla45531_2169.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG724457.
ProtClustDB	PRK13625.
Family and domain databases
HAMAP	MF_01443. PrpE. [Tree]
InterPro	IPR023937. Bis(5'-nucleosyl)-tetraP_PrpE. IPR004843. Metallo_PEstase_dom. IPR006186. Ser/Thr-sp_prot-phosphatase. [Graphical view]
KO	K01090.
Pfam	PF00149. Metallophos. 1 hit. [Graphical view]
PRINTS	PR00114. STPHPHTASE.
ProtoNet	Search...

Entry information

Entry name

PRPE_ANOFW

Accession

Primary (citable) accession number: B7GLK2

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	February 10, 2009
Last modified:	January 25, 2012
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program