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Reviewed, UniProtKB/Swiss-Prot B7GL27 (PGK_ANOFW)

Last modified July 28, 2009. Version 7. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Phosphoglycerate kinase
    EC=2.7.2.3
Gene names
Name: pgk
Ordered Locus Names: Aflv_2517
OrganismAnoxybacillus flavithermus (strain DSM 21510 / WK1) [Complete proteome] [HAMAP]
Taxonomic identifier491915 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacillales › Bacillaceae › Anoxybacillus

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Protein attributes

Sequence length394 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceInferred from homology.

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General annotation (Comments)

Catalytic activity

ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate. HAMAP MF_00145

Pathway

Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 2/5. HAMAP MF_00145

Subunit structure

Monomer By similarity.

Subcellular location

Cytoplasm Potential.

Sequence similarities

Belongs to the phosphoglycerate kinase family.

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Ontologies

Keywords
   Biological processGlycolysis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionKinase
Transferase
   PTMPhosphoprotein
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processglycolysis

Inferred from electronic annotation. Source: HAMAP

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

phosphoglycerate kinase activity

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 394394Phosphoglycerate kinase HAMAP MF_00145
PRO_1000192792

Regions

Nucleotide binding350 – 3534ATP By similarity
Region21 – 233Substrate binding By similarity
Region59 – 624Substrate binding By similarity

Sites

Binding site361Substrate By similarity
Binding site1181Substrate By similarity
Binding site1511Substrate By similarity
Binding site2011ATP By similarity
Binding site2921ATP; via carbonyl oxygen By similarity
Binding site3231ATP By similarity

Amino acid modifications

Modified residue1831Phosphoserine By similarity
Modified residue2991Phosphothreonine By similarity

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Sequences

Sequence LengthMass (Da)Tools
B7GL27-1 [UniParc].

Last modified February 10, 2009. Version 1.
Checksum: D090BB7725A9482A

FASTA39442,686
        10         20         30         40         50         60 
MNKKTVRDID VKGKRVFCRV DFNVPMQNGV VTDDTRIRAA LPTIQYLMEQ GAKVILASHL 

        70         80         90        100        110        120 
GRPKGKVVEE MRLNAVAERL SELLGKRVVK TDEAYGDTVK AAIAEMNEGD VLLLENVRFY 

       130        140        150        160        170        180 
PGEEKNDPEL AKAFAELADV YVNDAFGAAH RAHASTEGIA HHLPAVAGFL MEKEIEVLGK 

       190        200        210        220        230        240 
ALSNPDRPFT AIIGGAKVKD KIGVIENLLD KVDNLIIGGG LAYTFVKALG HEIGKSLLEE 

       250        260        270        280        290        300 
DKIDLAKSFM EKAKEKGVNF YMPVDAVVAD DFSNDANKKV VNIDEIPSDW EGLDIGPKTR 

       310        320        330        340        350        360 
ELYRDVILKS KLVIWNGPMG VFEMDAFAEG TKAVAQALAD AKDTYTVIGG GDSAAAVEKF 

       370        380        390 
GLANKMDHIS TGGGASLEFM EGKQLPGVVA LNDK

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References

[1]"Encapsulated in silica: genome, proteome and physiology of the thermophilic bacterium Anoxybacillus flavithermus WK1."
Saw J.H., Mountain B.W., Feng L., Omelchenko M.V., Hou S., Saito J.A., Stott M.B., Li D., Zhao G., Wu J., Galperin M.Y., Koonin E.V., Makarova K.S., Wolf Y.I., Rigden D.J., Dunfield P.F., Wang L., Alam M.
Genome Biol. 9:RESEARCH161.1-RESEARCH161.16(2008) [PubMed: 19014707] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

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Cross-references

Sequence databases

CP000922 Genomic DNA. Translation: ACJ34872.1.
RefSeqYP_002316857.1.

3D structure databases

ModBaseSearch...

Genome annotation databases

GeneID7038790.
GenomeReviewsGene locus Aflv_2517 in contig CP000922_GR.
KEGGafl:Aflv_2517.

Organism-specific databases

CMRSearch...

Family and domain databases

HAMAPMF_00145.
[Tree]
InterProIPR001576. Phosphoglycerate_kinase.
IPR015901. Phosphoglycerate_kinase_C.
IPR015911. Phosphoglycerate_kinase_CS.
IPR015824. Phosphoglycerate_kinase_N.
[Graphical view]
Gene3DG3DSA:3.40.50.1270. Phosphoglycerate_kinase_C. 1 hit.
G3DSA:3.40.50.1260. Phosphoglycerate_kinase_N. 1 hit.
PANTHERPTHR11406. PGK. 1 hit.
PfamPF00162. PGK. 1 hit.
[Graphical view]
PRINTSPR00477. PHGLYCKINASE.
PROSITEPS00111. PGLYCERATE_KINASE. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry namePGK_ANOFW
AccessionPrimary (citable) accession number: B7GL27
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: February 10, 2009
Last modified: July 28, 2009
This is version 7 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents