Reviewed, UniProtKB/Swiss-Prot B7GK78 (MURC_ANOFW)

Names and origin

Protein names

Recommended name:
    UDP-N-acetylmuramate--L-alanine ligase
    EC=6.3.2.8
Alternative name(s):
    UDP-N-acetylmuramoyl-L-alanine synthetase

Gene names

Name:	murC
Ordered Locus Names:	Aflv_0451

Organism

Anoxybacillus flavithermus (strain DSM 21510 / WK1) [Complete proteome] [HAMAP]

Taxonomic identifier

491915 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Anoxybacillus

Protein attributes

Sequence length	433 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cell wall formation By similarity.
Catalytic activity	ATP + UDP-N-acetylmuramate + L-alanine = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanine. HAMAP MF_00046
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00046
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the murCDEF family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP UDP-N-acetylmuramate-L-alanine ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 433	433	UDP-N-acetylmuramate--L-alanine ligase HAMAP MF_00046		PRO_1000116614
Regions
	Nucleotide binding	108 – 114	7	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	B7GK78-1 [UniParc]. Last modified February 10, 2009. Version 1. Checksum: 12617051EF25E82F	FASTA	433	48,992
	10 20 30 40 50 60 MTIYHFVGIK GTGMSALAQV LHDMKCVVQG SDYDKRFFTQ EALEARGIPI FPFSAQNIRE 70 80 90 100 110 120 NMVVIAGNAF PDTHEEIVAA RELGVPVIRY HQFLGDFLQK FTSIAVTGAH GKTSTTGLLA 130 140 150 160 170 180 HVMQGAKPTS YLIGDGSGKG REGSEYFVFE ACEYRRHFLA YSPDYAIMTN IDFDHPDYFA 190 200 210 220 230 240 NIEDVFSAFQ QMAWQVKKGI IACGDDEYLQ KIQAKVPVVF YGFGEDNDFQ ARNIVKTPEG 250 260 270 280 290 300 TSFDVFVRHT FFASFHIPRY GDHNVLNALA VIALCHYEGI DVNIIQERLK TFPGVKRRFH 310 320 330 340 350 360 EKMLGSQVLI DDYAHHPTEI RATIEAARQK YPERQIVAIF QPHTYTRTQT FLDEFAESLS 370 380 390 400 410 420 LADAVYLCDI FSSAREHQGK LSIEDLRAKI EGAKLLDEEQ VDVLKQHDNA VLIFMGAGDI 430 QKFQEKYEQS VHV

References

[1]

"Encapsulated in silica: genome, proteome and physiology of the thermophilic bacterium Anoxybacillus flavithermus WK1." Saw J.H., Mountain B.W., Feng L., Omelchenko M.V., Hou S., Saito J.A., Stott M.B., Li D., Zhao G., Wu J., Galperin M.Y., Koonin E.V., Makarova K.S., Wolf Y.I., Rigden D.J., Dunfield P.F., Wang L., Alam M. Genome Biol. 9:RESEARCH161.1-RESEARCH161.16(2008) [PubMed: 19014707] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000922 Genomic DNA. Translation: ACJ32835.1.
RefSeq	YP_002314820.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	7036708.
GenomeReviews	Gene locus Aflv_0451 in contig CP000922_GR.
KEGG	afl:Aflv_0451.
Organism-specific databases
CMR	Search...
Family and domain databases
HAMAP	MF_00046. [Tree]
InterPro	IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR000713. Mur_ligase_N. IPR016040. NAD(P)-bd_dom. IPR005758. UDP-N-AcMur_Ala_ligase. [Graphical view]
Gene3D	G3DSA:3.90.190.20. Mur_ligase_C. 1 hit. G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF01225. Mur_ligase. 1 hit. PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
TIGRFAMs	TIGR01082. murC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURC_ANOFW

Accession

Primary (citable) accession number: B7GK78

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	February 10, 2009
Last modified:	October 13, 2009
This is version 8 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)