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B7GK53

- SYL_ANOFW

UniProt

B7GK53 - SYL_ANOFW

Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Anoxybacillus flavithermus (strain DSM 21510 / WK1)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 42 (01 Oct 2014)
      Sequence version 1 (10 Feb 2009)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei579 – 5791ATPUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. leucine-tRNA ligase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. leucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciAFLA491915:GHEO-500-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
    Alternative name(s):
    Leucyl-tRNA synthetaseUniRule annotation
    Short name:
    LeuRSUniRule annotation
    Gene namesi
    Name:leuSUniRule annotation
    Ordered Locus Names:Aflv_0425
    OrganismiAnoxybacillus flavithermus (strain DSM 21510 / WK1)
    Taxonomic identifieri491915 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeAnoxybacillus
    ProteomesiUP000000742: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 805805Leucine--tRNA ligasePRO_1000199172Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi491915.Aflv_0425.

    Structurei

    3D structure databases

    ProteinModelPortaliB7GK53.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi40 – 5112"HIGH" regionAdd
    BLAST
    Motifi576 – 5805"KMSKS" region

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0495.
    HOGENOMiHOG000200748.
    KOiK01869.
    OMAiHISHELW.
    OrthoDBiEOG63Z74X.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_00049_B. Leu_tRNA_synth_B.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002302. Leu-tRNA-ligase.
    IPR025709. Leu_tRNA-synth_edit.
    IPR015413. Methionyl/Leucyl_tRNA_Synth.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF13603. tRNA-synt_1_2. 1 hit.
    PF09334. tRNA-synt_1g. 1 hit.
    [Graphical view]
    PRINTSiPR00985. TRNASYNTHLEU.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    B7GK53-1 [UniParc]FASTAAdd to Basket

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    MSFNHREIEK KWQKYWEENK TFKTTEDDGK RKFYALDMFP YPSGAGLHVG    50
    HPEGYTATDI LARMKRMQGY NVLHPMGWDA FGLPAEQYAL DTGNDPAEFT 100
    EKNINNFRRQ IKSLGFSYDW DREVNTTDPN YYKWTQWIFL KLYEKGLAYM 150
    DEVPVNWCPA LGTVLANEEV IDGKSERGGH PVIRKPMKQW MLRITAYADR 200
    LLEDLEELDW PESIKEMQRN WIGRSEGANI HFQVDGHDET FTVFTTRPDT 250
    LFGATYAVLA PEHPLVEKIT TPEQKEAVEA YLKQIQSKSD LERTDLAKEK 300
    TGVFTGAYAI NPANGEKLPI WIADYVLMSY GTGAIMAVPA HDERDYEFAK 350
    KFNLPIKQVV AGGDISKEAY TGDGEHINSD FLNGLNKEEA TKKMIEWLEA 400
    NGKGEKKVSY RLRDWLFSRQ RYWGEPIPII HWEDGTMTPV PEEELPLVLP 450
    KTDEIKPSGT GESPLANIEE WVSVVDPKTG KKGRRETNTM PQWAGSCWYY 500
    LRYIDPHNDK QLADPEKLEK WLPVDIYIGG AEHAVLHLLY ARFWHKFLYD 550
    IGVVPTKEPF QKLFNQGMIL GENNEKMSKS KGNVVNPDDI VESHGADTLR 600
    LYEMFMGPLE ASIAWSTKGL DGARRFLDRV WRLFVEENGE LNPKIVDNPE 650
    TDTLERVYHQ TVKKVTEDYE ALRFNTAISQ LMVFINEAYK APILPKAYME 700
    GFVKLLSPVC PHIAEELWEK LGYSNTIAYE AWPAYDEAKL VEDEVEIVIQ 750
    VNGKVRAKLH VPADATKEQL EQLAMEDEKI KEQIEGKTVR KVIAVPGKLV 800
    NIVAN 805
    Length:805
    Mass (Da):92,398
    Last modified:February 10, 2009 - v1
    Checksum:iA208849E5C1DC81C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000922 Genomic DNA. Translation: ACJ32809.1.
    RefSeqiYP_002314794.1. NC_011567.1.

    Genome annotation databases

    EnsemblBacteriaiACJ32809; ACJ32809; Aflv_0425.
    GeneIDi7036682.
    KEGGiafl:Aflv_0425.
    PATRICi20952647. VBIAnoFla45531_0435.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000922 Genomic DNA. Translation: ACJ32809.1 .
    RefSeqi YP_002314794.1. NC_011567.1.

    3D structure databases

    ProteinModelPortali B7GK53.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 491915.Aflv_0425.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACJ32809 ; ACJ32809 ; Aflv_0425 .
    GeneIDi 7036682.
    KEGGi afl:Aflv_0425.
    PATRICi 20952647. VBIAnoFla45531_0435.

    Phylogenomic databases

    eggNOGi COG0495.
    HOGENOMi HOG000200748.
    KOi K01869.
    OMAi HISHELW.
    OrthoDBi EOG63Z74X.

    Enzyme and pathway databases

    BioCyci AFLA491915:GHEO-500-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_00049_B. Leu_tRNA_synth_B.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002302. Leu-tRNA-ligase.
    IPR025709. Leu_tRNA-synth_edit.
    IPR015413. Methionyl/Leucyl_tRNA_Synth.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF13603. tRNA-synt_1_2. 1 hit.
    PF09334. tRNA-synt_1g. 1 hit.
    [Graphical view ]
    PRINTSi PR00985. TRNASYNTHLEU.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00396. leuS_bact. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Encapsulated in silica: genome, proteome and physiology of the thermophilic bacterium Anoxybacillus flavithermus WK1."
      Saw J.H., Mountain B.W., Feng L., Omelchenko M.V., Hou S., Saito J.A., Stott M.B., Li D., Zhao G., Wu J., Galperin M.Y., Koonin E.V., Makarova K.S., Wolf Y.I., Rigden D.J., Dunfield P.F., Wang L., Alam M.
      Genome Biol. 9:R161.1-R161.16(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: DSM 21510 / WK1.

    Entry informationi

    Entry nameiSYL_ANOFW
    AccessioniPrimary (citable) accession number: B7GK53
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 14, 2009
    Last sequence update: February 10, 2009
    Last modified: October 1, 2014
    This is version 42 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3