Reviewed, UniProtKB/Swiss-Prot B7GJH1 (PROA_ANOFW)

Names and origin

Protein names

Recommended name:
    Gamma-glutamyl phosphate reductase
      Short name=GPR
    EC=1.2.1.41
Alternative name(s):
    Glutamate-5-semialdehyde dehydrogenase
    Glutamyl-gamma-semialdehyde dehydrogenase
      Short name=GSA dehydrogenase

Gene names

Name:	proA
Ordered Locus Names:	Aflv_1271

Organism

Anoxybacillus flavithermus (strain DSM 21510 / WK1) [Complete proteome] [HAMAP]

Taxonomic identifier

491915 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Anoxybacillus

Protein attributes

Sequence length	413 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the NADPH dependent reduction of L-gamma-glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate By similarity. HAMAP MF_00412
Catalytic activity	L-glutamate 5-semialdehyde + phosphate + NADP+ = L-glutamyl 5-phosphate + NADPH. HAMAP MF_00412
Pathway	Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 2/2. HAMAP MF_00412
Subcellular location	Cytoplasm By similarity HAMAP MF_00412.
Sequence similarities	Belongs to the gamma-glutamyl phosphate reductase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Proline biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW proline biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NADP or NADPH binding Inferred from electronic annotation. Source: InterPro glutamate-5-semialdehyde dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 413	413	Gamma-glutamyl phosphate reductase HAMAP MF_00412		PRO_1000123774

Sequences

	Sequence		Length	Mass (Da)
	B7GJH1-1 [UniParc]. Last modified February 10, 2009. Version 1. Checksum: D6E2DB0A7E3A61E4	FASTA	413	45,612
	10 20 30 40 50 60 MSELIVKAKK LKQAASQLAM LSTEEKNNAL AMIAEALIAQ TGYILQENEK DMEIGKENGL 70 80 90 100 110 120 SPSLLDRLQL TEERVQQIAD GVRQVVDLPD PIGETIEQWT RPNGLILKQI RVPLGVVGMV 130 140 150 160 170 180 YEARPNVTVD AASLCLKTGN AVLLRGSSSA IHSNKALISV MQQALKQSKI PTDAIQLLED 190 200 210 220 230 240 TSRETAQQMF RLKEYIDVLI PRGGAGLIQS VVENATIPVL ETGVGNCHIF IDDSAQKEMA 250 260 270 280 290 300 IDIVINAKLQ RPSVCNAVET VIVHKQWPYI KELLETLHEK GVELRADKQL ADTYPFVHEA 310 320 330 340 350 360 KEEDWATEFL APILAVKLVE TVDEAIEHIE RYGTKHSEAI ISECNEHVEQ FFARVDAAVL 370 380 390 400 410 YHNASTRFTD GEQFGYGAEI GISTQKLHAR GPMGLRAITT TKTLVYGSGQ VRK

References

[1]

"Encapsulated in silica: genome, proteome and physiology of the thermophilic bacterium Anoxybacillus flavithermus WK1." Saw J.H., Mountain B.W., Feng L., Omelchenko M.V., Hou S., Saito J.A., Stott M.B., Li D., Zhao G., Wu J., Galperin M.Y., Koonin E.V., Makarova K.S., Wolf Y.I., Rigden D.J., Dunfield P.F., Wang L., Alam M. Genome Biol. 9:R161.1-R161.16(2008) [PubMed: 19014707] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000922 Genomic DNA. Translation: ACJ33641.1.
RefSeq	YP_002315626.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	7037525.
GenomeReviews	Gene locus Aflv_1271 in contig CP000922_GR.
KEGG	afl:Aflv_1271.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG318080.
Family and domain databases
HAMAP	MF_00412. ProA. [Tree]
InterPro	IPR016161. Ald_DH/histidinol_DH. IPR016163. Ald_DH_C. IPR016162. Ald_DH_N. IPR015590. Aldehyde_DH. IPR000965. G-glutamylP_reductase. IPR020593. G-glutamylP_reductase_CS. IPR012134. Glu-5-SA_DH. [Graphical view]
Gene3D	G3DSA:3.40.309.10. Aldehyde_dehydrogenase_C. 1 hit. G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
PANTHER	PTHR11063:SF1. GSA_DH. 1 hit.
Pfam	PF00171. Aldedh. 1 hit. [Graphical view]
PIRSF	PIRSF000151. GPR. 1 hit.
TIGRFAMs	TIGR00407. proA. 1 hit.
PROSITE	PS01223. PROA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PROA_ANOFW

Accession

Primary (citable) accession number: B7GJH1

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	February 10, 2009
Last modified:	February 9, 2010
This is version 11 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)