B7GJ46 (SYC_ANOFW) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cysteine--tRNA ligase
EC=6.1.1.16

Alternative name(s):
Cysteinyl-tRNA synthetase
Short name=CysRS

Gene names

Name:	cysS
Ordered Locus Names:	Aflv_0084

Organism

Anoxybacillus flavithermus (strain DSM 21510 / WK1) [Complete proteome] [HAMAP]

Taxonomic identifier

491915 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Anoxybacillus

Protein attributes

Sequence length	465 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys). HAMAP MF_00041
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00041
Subunit structure	Monomer By similarity. HAMAP MF_00041
Subcellular location	Cytoplasm By similarity HAMAP MF_00041.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cysteinyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW cysteine-tRNA ligase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 465	465	Cysteine--tRNA ligase HAMAP MF_00041		PRO_1000199032
Regions
	Motif	31 – 41	11	"HIGH" region HAMAP MF_00041
	Motif	266 – 270	5	"KMSKS" region HAMAP MF_00041
Sites
	Metal binding	29	1	Zinc By similarity
	Metal binding	209	1	Zinc By similarity
	Metal binding	234	1	Zinc By similarity
	Metal binding	238	1	Zinc By similarity
	Binding site	269	1	ATP By similarity
Amino acid modifications
	Modified residue	270	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	B7GJ46 [UniParc]. Last modified February 10, 2009. Version 1. Checksum: FAA1D6C5D7C68D49	FASTA	465	54,009
	10 20 30 40 50 60 MSIQVYNTLT RKKEPFIPLE PNKVKMYVCG PTVYNYIHIG NARPAIVFDT IRRYLQFRGY 70 80 90 100 110 120 EVQYVSNFTD VDDKLIRAAR ELGEDVPTIA ERFIEAYFED ITALGCKKAD VHPRVTENID 130 140 150 160 170 180 TIIQFIEQLI EKGYAYEVDG DVYYRTRRFS DYGKLSHQSV DELKSGARIE VGEKKEDPLD 190 200 210 220 230 240 FALWKAAKEG EIYWDSPWGK GRPGWHIECS AMARKYLGDT IDIHAGGQDL TFPHHENEIA 250 260 270 280 290 300 QSEALTGKPF AKYWLHNGYI NIDNEKMSKS LGNFILVHDI IKQVDPQVLR FFMLSVHYRH 310 320 330 340 350 360 PINYSQPLLE SARSGLERLK TAYANLKHRL ESSTNLTTND EQWLAKIEEL RNEFIREMDD 370 380 390 400 410 420 DFNTANGIAV LFELAKQANV YLMENHTSQQ VIQAFLQQFE QLFDVLGLSL QQDELLDEEI 430 440 450 460 EALIQQRIEA RKNRDFALAD RIRDELKAKN IILEDTPQGT RWRRG

References

[1]

"Encapsulated in silica: genome, proteome and physiology of the thermophilic bacterium Anoxybacillus flavithermus WK1." Saw J.H., Mountain B.W., Feng L., Omelchenko M.V., Hou S., Saito J.A., Stott M.B., Li D., Zhao G., Wu J., Galperin M.Y., Koonin E.V., Makarova K.S., Wolf Y.I., Rigden D.J., Dunfield P.F., Wang L., Alam M. Genome Biol. 9:R161.1-R161.16(2008) [PubMed: 19014707] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 21510 / WK1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000922 Genomic DNA. Translation: ACJ32468.1.
RefSeq	YP_002314453.1. NC_011567.1.
3D structure databases
ProteinModelPortal	B7GJ46.
ModBase	Search...
Protein-protein interaction databases
STRING	B7GJ46.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7036283.
GenomeReviews	Gene locus Aflv_0084 in contig CP000922_GR.
KEGG	afl:Aflv_0084.
PATRIC	20951831. VBIAnoFla45531_0084.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG327651.
ProtClustDB	PRK00260.
Family and domain databases
HAMAP	MF_00041. Cys_tRNA_synth. [Tree]
InterPro	IPR015803. Cys-tRNA-synt. IPR015273. Cys-tRNA-synt_Ia_DALR. IPR024909. Cys-tRNA/MSH_ligase. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits.
KO	K01883.
PANTHER	PTHR10890. Cys_tRNA-synt_1a. 1 hit.
Pfam	PF09190. DALR_2. 1 hit. PF01406. tRNA-synt_1e. 1 hit. [Graphical view]
PRINTS	PR00983. TRNASYNTHCYS.
SMART	SM00840. DALR_2. 1 hit. [Graphical view]
SUPFAM	SSF47323. tRNAsyn_1a_bind. 1 hit.
TIGRFAMs	TIGR00435. CysS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYC_ANOFW

Accession

Primary (citable) accession number: B7GJ46

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	February 10, 2009
Last modified:	January 25, 2012
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program