B7GH35 (GSA2_ANOFW) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate-1-semialdehyde 2,1-aminomutase 2
Short name=GSA 2
EC=5.4.3.8

Alternative name(s):
Glutamate-1-semialdehyde aminotransferase 2
Short name=GSA-AT 2

Gene names

Name:	hemL2
Ordered Locus Names:	Aflv_0610

Organism

Anoxybacillus flavithermus (strain DSM 21510 / WK1) [Complete proteome] [HAMAP]

Taxonomic identifier

491915 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Anoxybacillus › Anoxybacillus flavithermus

Protein attributes

Sequence length	431 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	(S)-4-amino-5-oxopentanoate = 5-aminolevulinate. HAMAP-Rule MF_00375
Cofactor	Pyridoxal phosphate By similarity.
Pathway	Porphyrin metabolism; protoporphyrin-IX biosynthesis; 5-aminolevulinate from L-glutamyl-tRNA(Glu): step 2/2. HAMAP-Rule MF_00375
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm Potential HAMAP-Rule MF_00375.
Sequence similarities	Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily.
Sequence caution	The sequence ACJ32991.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Porphyrin biosynthesis
Cellular component	Cytoplasm
Ligand	Pyridoxal phosphate
Molecular function	Isomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	chlorophyll biosynthetic process Inferred from electronic annotation. Source: HAMAP protoporphyrinogen IX biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	glutamate-1-semialdehyde 2,1-aminomutase activity Inferred from electronic annotation. Source: HAMAP pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro transaminase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 431	431	Glutamate-1-semialdehyde 2,1-aminomutase 2 HAMAP-Rule MF_00375		PRO_0000382251
Amino acid modifications
	Modified residue	268	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	B7GH35 [UniParc]. Last modified September 1, 2009. Version 2. Checksum: 6F6DBDBBAAE864F7	FASTA	431	46,825
	10 20 30 40 50 60 MRSYERSKAA YAEAVKLMPG GVNSPVRAFK AVKMDPIFMA RGKGSKIYDI DGNEYIDYVL 70 80 90 100 110 120 SWGPLILGHA NDQVVEALKR VAETGTSFGA PTLIENELAK LVMERMPSIE IIRMVSSGTE 130 140 150 160 170 180 ATMSALRLAR GYTGRNKIVK FEGCYHGHGD SLLIKAGSGV ATLGLPDSPG VPETVAQHTI 190 200 210 220 230 240 TVPYNDLQSV RYAFEKFGED IAAVIVEPVA GNMGVVPPEP GFLQGLRDVT NEYGALLIFD 250 260 270 280 290 300 EVMTGFRVDY YSGQGYYGVT PDLTCLGKVI GGGLPVGAYG GRADIMEKIA PSGPIYQAGT 310 320 330 340 350 360 LSGNPMAMTA GYETLRQLTP ETYEAFKKKA NRLAEGLSEA AKAYHIPHTI NQAGSMIGMF 370 380 390 400 410 420 FTNERVTNYE TAKTSNLDLF ARYYQEMANE GIFLPPSQFE GMFLSTAHTD EDIEKTIEAA 430 RRAFAKMSDC L

References

[1]

"Encapsulated in silica: genome, proteome and physiology of the thermophilic bacterium Anoxybacillus flavithermus WK1." Saw J.H., Mountain B.W., Feng L., Omelchenko M.V., Hou S., Saito J.A., Stott M.B., Li D., Zhao G., Wu J., Galperin M.Y., Koonin E.V., Makarova K.S., Wolf Y.I., Rigden D.J., Dunfield P.F., Wang L., Alam M. Genome Biol. 9:R161.1-R161.16(2008) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 21510 / WK1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000922 Genomic DNA. Translation: ACJ32991.1. Different initiation.
RefSeq	YP_002314976.1. NC_011567.1.
3D structure databases
ProteinModelPortal	B7GH35.
ModBase	Search...
Protein-protein interaction databases
STRING	491915.Aflv_0610.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACJ32991; ACJ32991; Aflv_0610.
GeneID	7036867.
KEGG	afl:Aflv_0610.
PATRIC	20953033. VBIAnoFla45531_0627.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0001.
HOGENOM	HOG000020210.
KO	K01845.
OMA	FNGNPIS.
ProtClustDB	PRK00062.
Enzyme and pathway databases
BioCyc	AFLA491915:GHEO-685-MONOMER.
UniPathway	UPA00251; UER00317.
Family and domain databases
Gene3D	3.40.640.10. 1 hit. 3.90.1150.10. 2 hits.
HAMAP	MF_00375. HemL_aminotrans_3.
InterPro	IPR004639. 4pyrrol_synth_GluAld_NH2Trfase. IPR005814. Aminotrans_3. IPR015424. PyrdxlP-dep_Trfase. IPR015421. PyrdxlP-dep_Trfase_major_sub1. IPR015422. PyrdxlP-dep_Trfase_major_sub2. [Graphical view]
PANTHER	PTHR11986. PTHR11986. 1 hit. PTHR11986:SF5. PTHR11986:SF5. 1 hit.
Pfam	PF00202. Aminotran_3. 1 hit. [Graphical view]
SUPFAM	SSF53383. PyrdxlP-dep_Trfase_major. 1 hit.
TIGRFAMs	TIGR00713. hemL. 1 hit.
PROSITE	PS00600. AA_TRANSFER_CLASS_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GSA2_ANOFW

Accession

Primary (citable) accession number: B7GH35

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 1, 2009
Last sequence update:	September 1, 2009
Last modified:	May 1, 2013
This is version 36 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program