Reviewed, UniProtKB/Swiss-Prot B7GGW8 (SYFA_ANOFW)

Names and origin

Protein names

Recommended name:
    Phenylalanyl-tRNA synthetase alpha chain
    EC=6.1.1.20
Alternative name(s):
    Phenylalanine--tRNA ligase alpha chain
      Short name=PheRS

Gene names

Name:	pheS
Ordered Locus Names:	Aflv_0542

Organism

Anoxybacillus flavithermus (strain DSM 21510 / WK1) [Complete proteome] [HAMAP]

Taxonomic identifier

491915 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Anoxybacillus

Protein attributes

Sequence length	344 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe). HAMAP MF_00281
Cofactor	Binds 2 magnesium ions per tetramer By similarity.
Subunit structure	Tetramer of two alpha and two beta chains By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phenylalanyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP phenylalanine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 344	344	Phenylalanyl-tRNA synthetase alpha chain HAMAP MF_00281		PRO_1000119385
Sites
	Metal binding	256	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	B7GGW8-1 [UniParc]. Last modified February 10, 2009. Version 1. Checksum: BC9EB0C7451CA12C	FASTA	344	39,009
	10 20 30 40 50 60 MKERLQQLQQ EALEKIEQAN DLKALNDVRV AYLGKKGPIT EVLRGMGALS PEERPIMGAL 70 80 90 100 110 120 VNDVREAIQQ ALEAKQATLE QAEVEKKLAA EAIDVTLPGR PIRRGNHHPL TRVIEEIEDL 130 140 150 160 170 180 FIGMGYTIAE GPEVEQDYYN FEALNLPKGH PARDMQDSFY ITEEILLRTH TSPVQARTME 190 200 210 220 230 240 KHQGRGPVKI ICPGKVYRRD NDDATHSHQF TQIEGLVVDE NIRMSDLKGT LREFARKMFG 250 260 270 280 290 300 EDRDIRFRPS FFPFTEPSVE VDVSCFNCGG HGCNVCKGTG WIEILGAGMV HPNVLEMAGF 310 320 330 340 DSKKYTGFAF GMGPERIAML KYGIDDIRHF YQNDLRFLQQ FNRV

References

[1]

"Encapsulated in silica: genome, proteome and physiology of the thermophilic bacterium Anoxybacillus flavithermus WK1." Saw J.H., Mountain B.W., Feng L., Omelchenko M.V., Hou S., Saito J.A., Stott M.B., Li D., Zhao G., Wu J., Galperin M.Y., Koonin E.V., Makarova K.S., Wolf Y.I., Rigden D.J., Dunfield P.F., Wang L., Alam M. Genome Biol. 9:RESEARCH161.1-RESEARCH161.16(2008) [PubMed: 19014707] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000922 Genomic DNA. Translation: ACJ32923.1.
RefSeq	YP_002314908.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	7036798.
GenomeReviews	Gene locus Aflv_0542 in contig CP000922_GR.
KEGG	afl:Aflv_0542.
Organism-specific databases
CMR	Search...
Family and domain databases
HAMAP	MF_00281. [Tree]
InterPro	IPR006195. aa-tRNA-synth_II_cons-reg. IPR004529. Phe-tRNA-synth_IIc_asu. IPR004188. Phe-tRNA_synth_II_N. IPR002319. Phenylalanyl-tRNA_Synthase_acu. [Graphical view]
PANTHER	PTHR11538. tRNA-synt_2d. 1 hit.
Pfam	PF02912. Phe_tRNA-synt_N. 1 hit. PF01409. tRNA-synt_2d. 1 hit. [Graphical view]
TIGRFAMs	TIGR00468. pheS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYFA_ANOFW

Accession

Primary (citable) accession number: B7GGW8

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	February 10, 2009
Last modified:	September 22, 2009
This is version 7 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)