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Protein

ATP-dependent 6-phosphofructokinase

Gene

pfkA

Organism
Anoxybacillus flavithermus (strain DSM 21510 / WK1)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Enzyme regulationi

Allosterically activated by ADP and other diphosphonucleosides, and allosterically inhibited by phosphoenolpyruvate.UniRule annotation

Pathwayi: glycolysis

This protein is involved in step 3 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.UniRule annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Glucose-6-phosphate isomerase (pgi)
  3. ATP-dependent 6-phosphofructokinase (pfkA)
  4. no protein annotated in this organism
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei11ATP; via amide nitrogenUniRule annotation1
Metal bindingi103Magnesium; catalyticUniRule annotation1
Active sitei127Proton acceptorUniRule annotation1
Binding sitei154Allosteric activator ADPUniRule annotation1
Binding sitei162Substrate; shared with dimeric partnerUniRule annotation1
Binding sitei211Allosteric activator ADPUniRule annotation1
Binding sitei222SubstrateUniRule annotation1
Binding sitei243Substrate; shared with dimeric partnerUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi72 – 73ATPUniRule annotation2
Nucleotide bindingi102 – 105ATPUniRule annotation4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAllosteric enzyme, Kinase, Transferase
Biological processGlycolysis
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciAFLA491915:G1GAW-584-MONOMER
UniPathwayiUPA00109; UER00182

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinaseUniRule annotation (EC:2.7.1.11UniRule annotation)
Short name:
ATP-PFKUniRule annotation
Short name:
PhosphofructokinaseUniRule annotation
Alternative name(s):
PhosphohexokinaseUniRule annotation
Gene namesi
Name:pfkAUniRule annotation
Ordered Locus Names:Aflv_0499
OrganismiAnoxybacillus flavithermus (strain DSM 21510 / WK1)
Taxonomic identifieri491915 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeAnoxybacillus
Proteomesi
  • UP000000742 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001200261 – 319ATP-dependent 6-phosphofructokinaseAdd BLAST319

Proteomic databases

PRIDEiB7GGT1

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi491915.Aflv_0499

Structurei

3D structure databases

ProteinModelPortaliB7GGT1
SMRiB7GGT1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni21 – 25Allosteric activator ADP binding; shared with dimeric partnerUniRule annotation5
Regioni125 – 127Substrate bindingUniRule annotation3
Regioni169 – 171Substrate bindingUniRule annotation3
Regioni185 – 187Allosteric activator ADP bindingUniRule annotation3
Regioni213 – 215Allosteric activator ADP bindingUniRule annotation3
Regioni249 – 252Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Prokaryotic clade "B1" sub-subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CTQ Bacteria
COG0205 LUCA
HOGENOMiHOG000248869
KOiK00850
OMAiGKLHSII
OrthoDBiPOG091H01AC

Family and domain databases

CDDicd00763 Bacterial_PFK, 1 hit
HAMAPiMF_00339 Phosphofructokinase_I_B1, 1 hit
InterProiView protein in InterPro
IPR022953 ATP_PFK
IPR012003 ATP_PFK_prok-type
IPR012828 PFKA_ATP_prok
IPR015912 Phosphofructokinase_CS
IPR000023 Phosphofructokinase_dom
IPR035966 PKF_sf
PfamiView protein in Pfam
PF00365 PFK, 1 hit
PIRSFiPIRSF000532 ATP_PFK_prok, 1 hit
PRINTSiPR00476 PHFRCTKINASE
SUPFAMiSSF53784 SSF53784, 1 hit
TIGRFAMsiTIGR02482 PFKA_ATP, 1 hit
PROSITEiView protein in PROSITE
PS00433 PHOSPHOFRUCTOKINASE, 1 hit

Sequencei

Sequence statusi: Complete.

B7GGT1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKRIGVLTSG GDSPGMNAAI RAVVRKAIYH GLEVYGIYHG YAGLIAGQIK
60 70 80 90 100
KLEIGDVGDI IHRGGTMLYT ARCPEFKTLE GQLKGIEQLK KHGIEGLVVI
110 120 130 140 150
GGDGSYQGAK KLTEHGYPCV GVPGTIDNDI PGTDFTIGFD TALNTVIDAI
160 170 180 190 200
DKIRDTATSH ERTYVIEVMG RHAGDIALWA GLAGGAESIL IPEADYDMDE
210 220 230 240 250
IVSRLKRGHE RGKKHSIIIV AEGVGSGVDF GKRIEEATGL ETRVTVLGHV
260 270 280 290 300
QRGGSPTAFD RVLASRLGAR AVELLIEGKG GRCVGIQNNQ LVDHDIIEAL
310
AQTHTVDQKM YQLSKELSI
Length:319
Mass (Da):34,174
Last modified:February 10, 2009 - v1
Checksum:iB87117BB2F29CE7D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000922 Genomic DNA Translation: ACJ32883.1
RefSeqiWP_012574203.1, NC_011567.1

Genome annotation databases

EnsemblBacteriaiACJ32883; ACJ32883; Aflv_0499
KEGGiafl:Aflv_0499
PATRICifig|491915.6.peg.511

Similar proteinsi

Entry informationi

Entry nameiPFKA_ANOFW
AccessioniPrimary (citable) accession number: B7GGT1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: February 10, 2009
Last modified: May 23, 2018
This is version 59 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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