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B7GGT1

- PFKA_ANOFW

UniProt

B7GGT1 - PFKA_ANOFW

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Protein
ATP-dependent 6-phosphofructokinase
Gene
pfkA, Aflv_0499
Organism
Anoxybacillus flavithermus (strain DSM 21510 / WK1)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis By similarity.UniRule annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

Cofactori

Magnesium By similarity.UniRule annotation

Enzyme regulationi

Allosterically activated by ADP and other diphosphonucleosides, and allosterically inhibited by phosphoenolpyruvate By similarity.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei11 – 111ATP; via amide nitrogen By similarity
Metal bindingi103 – 1031Magnesium; catalytic By similarity
Active sitei127 – 1271Proton acceptor By similarity
Binding sitei154 – 1541Allosteric activator ADP By similarity
Binding sitei162 – 1621Substrate; shared with dimeric partner By similarity
Binding sitei211 – 2111Allosteric activator ADP By similarity
Binding sitei222 – 2221Substrate By similarity
Binding sitei243 – 2431Substrate; shared with dimeric partner By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi72 – 732ATP By similarity
Nucleotide bindingi102 – 1054ATP By similarity

GO - Molecular functioni

  1. 6-phosphofructokinase activity Source: UniProtKB-HAMAP
  2. ATP binding Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

GO - Biological processi

  1. fructose 6-phosphate metabolic process Source: InterPro
  2. glycolytic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciAFLA491915:GHEO-574-MONOMER.
UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinase (EC:2.7.1.11)
Short name:
ATP-PFK
Short name:
Phosphofructokinase
Alternative name(s):
Phosphohexokinase
Gene namesi
Name:pfkA
Ordered Locus Names:Aflv_0499
OrganismiAnoxybacillus flavithermus (strain DSM 21510 / WK1)
Taxonomic identifieri491915 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeAnoxybacillus
ProteomesiUP000000742: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. 6-phosphofructokinase complex Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 319319ATP-dependent 6-phosphofructokinaseUniRule annotation
PRO_1000120026Add
BLAST

Proteomic databases

PRIDEiB7GGT1.

Interactioni

Subunit structurei

Homotetramer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi491915.Aflv_0499.

Structurei

3D structure databases

ProteinModelPortaliB7GGT1.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni21 – 255Allosteric activator ADP binding; shared with dimeric partner By similarity
Regioni125 – 1273Substrate binding By similarity
Regioni169 – 1713Substrate binding By similarity
Regioni185 – 1873Allosteric activator ADP binding By similarity
Regioni213 – 2153Allosteric activator ADP binding By similarity
Regioni249 – 2524Substrate binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0205.
HOGENOMiHOG000248869.
KOiK00850.
OMAiGFGGRCV.
OrthoDBiEOG644ZRM.

Family and domain databases

HAMAPiMF_00339. Phosphofructokinase_I_B1.
InterProiIPR012003. ATP_PFK_prok.
IPR012828. PFKA_ATP.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
TIGRFAMsiTIGR02482. PFKA_ATP. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B7GGT1-1 [UniParc]FASTAAdd to Basket

« Hide

MKRIGVLTSG GDSPGMNAAI RAVVRKAIYH GLEVYGIYHG YAGLIAGQIK    50
KLEIGDVGDI IHRGGTMLYT ARCPEFKTLE GQLKGIEQLK KHGIEGLVVI 100
GGDGSYQGAK KLTEHGYPCV GVPGTIDNDI PGTDFTIGFD TALNTVIDAI 150
DKIRDTATSH ERTYVIEVMG RHAGDIALWA GLAGGAESIL IPEADYDMDE 200
IVSRLKRGHE RGKKHSIIIV AEGVGSGVDF GKRIEEATGL ETRVTVLGHV 250
QRGGSPTAFD RVLASRLGAR AVELLIEGKG GRCVGIQNNQ LVDHDIIEAL 300
AQTHTVDQKM YQLSKELSI 319
Length:319
Mass (Da):34,174
Last modified:February 10, 2009 - v1
Checksum:iB87117BB2F29CE7D
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000922 Genomic DNA. Translation: ACJ32883.1.
RefSeqiYP_002314868.1. NC_011567.1.

Genome annotation databases

EnsemblBacteriaiACJ32883; ACJ32883; Aflv_0499.
GeneIDi7036756.
KEGGiafl:Aflv_0499.
PATRICi20952799. VBIAnoFla45531_0511.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000922 Genomic DNA. Translation: ACJ32883.1 .
RefSeqi YP_002314868.1. NC_011567.1.

3D structure databases

ProteinModelPortali B7GGT1.
ModBasei Search...

Protein-protein interaction databases

STRINGi 491915.Aflv_0499.

Proteomic databases

PRIDEi B7GGT1.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACJ32883 ; ACJ32883 ; Aflv_0499 .
GeneIDi 7036756.
KEGGi afl:Aflv_0499.
PATRICi 20952799. VBIAnoFla45531_0511.

Phylogenomic databases

eggNOGi COG0205.
HOGENOMi HOG000248869.
KOi K00850.
OMAi GFGGRCV.
OrthoDBi EOG644ZRM.

Enzyme and pathway databases

UniPathwayi UPA00109 ; UER00182 .
BioCyci AFLA491915:GHEO-574-MONOMER.

Family and domain databases

HAMAPi MF_00339. Phosphofructokinase_I_B1.
InterProi IPR012003. ATP_PFK_prok.
IPR012828. PFKA_ATP.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view ]
Pfami PF00365. PFK. 1 hit.
[Graphical view ]
PIRSFi PIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSi PR00476. PHFRCTKINASE.
SUPFAMi SSF53784. SSF53784. 1 hit.
TIGRFAMsi TIGR02482. PFKA_ATP. 1 hit.
PROSITEi PS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Encapsulated in silica: genome, proteome and physiology of the thermophilic bacterium Anoxybacillus flavithermus WK1."
    Saw J.H., Mountain B.W., Feng L., Omelchenko M.V., Hou S., Saito J.A., Stott M.B., Li D., Zhao G., Wu J., Galperin M.Y., Koonin E.V., Makarova K.S., Wolf Y.I., Rigden D.J., Dunfield P.F., Wang L., Alam M.
    Genome Biol. 9:R161.1-R161.16(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 21510 / WK1.

Entry informationi

Entry nameiPFKA_ANOFW
AccessioniPrimary (citable) accession number: B7GGT1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: February 10, 2009
Last modified: July 9, 2014
This is version 38 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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