Reviewed, UniProtKB/Swiss-Prot B7GGN9 (SYT_ANOFW)

Names and origin

Protein names

Recommended name:
    Threonyl-tRNA synthetase
    EC=6.1.1.3
Alternative name(s):
    Threonine--tRNA ligase
      Short name=ThrRS

Gene names

Name:	thrS
Ordered Locus Names:	Aflv_0521

Organism

Anoxybacillus flavithermus (strain DSM 21510 / WK1) [Complete proteome] [HAMAP]

Taxonomic identifier

491915 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Anoxybacillus

Protein attributes

Sequence length	643 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-threonine + tRNA(Thr) = AMP + diphosphate + L-threonyl-tRNA(Thr). HAMAP MF_00184
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	threonyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP threonine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 643	643	Threonyl-tRNA synthetase HAMAP MF_00184		PRO_1000199526
Regions
	Region	245 – 542	298	Catalytic HAMAP MF_00184
Sites
	Metal binding	338	1	Zinc; catalytic By similarity
	Metal binding	389	1	Zinc; catalytic By similarity
	Metal binding	519	1	Zinc; catalytic By similarity

Sequences

	Sequence		Length	Mass (Da)
	B7GGN9-1 [UniParc]. Last modified February 10, 2009. Version 1. Checksum: 76CB0798FA115C22	FASTA	643	73,967
	10 20 30 40 50 60 MADVVKITFP DGAVKEFPKG VTTEDIAASI SPGLKKKAIA GKLNERMIDL RTPIEEDGAI 70 80 90 100 110 120 SIITQDMPEA LDILRHSTAH LMAQAVKRLY KDVKLGVGPV IENGFYYDID VDVPITAEDL 130 140 150 160 170 180 PKIEQEMKKI VKENLDIVRK EVSREEAIRR YEEIGDPLKI ELINDIPEGE VVSIYEQGEF 190 200 210 220 230 240 FDLCRGVHVP STGKIKEFKL LSVAGAYWRG DSKNKMLQRI YGTAFFKKED LDEYLRLLQE 250 260 270 280 290 300 AKERDHRKLG KELDLFMTSQ KVGQGLPLWL PKGATIRRTI ERYIVDKELS LGYQHVYTPV 310 320 330 340 350 360 LGSVELYKTS GHWDHYKDGM FPPMEMDNEQ LVLRPMNCPH HMMVYKHKIH SYRELPIRIA 370 380 390 400 410 420 ELGTMHRYEM SGALTGLQRV RGMTLNDAHI FVRPDQIKDE FKRVVNLILD VYKDFGLTDY 430 440 450 460 470 480 SFRLSYRDPQ DKEKYYDDDA MWEKAQSMLK EAMDELGLDY YEAEGEAAFY GPKLDVQVRT 490 500 510 520 530 540 ALGKDETLST VQLDFLLPER FDLTYIGEDG KPHRPVVIHR GVVSTMERFV AFLIEEYKGA 550 560 570 580 590 600 FPTWLAPIQV QVIPVAPSIH MDYAYEVKHA LQVAGIRVEV DEREEKIGYK IREAQMQKIP 610 620 630 640 YMLVVGDNEV NERAVNVRKY GEQKSETMPL DAFVDSIAKE VRK

References

[1]

"Encapsulated in silica: genome, proteome and physiology of the thermophilic bacterium Anoxybacillus flavithermus WK1." Saw J.H., Mountain B.W., Feng L., Omelchenko M.V., Hou S., Saito J.A., Stott M.B., Li D., Zhao G., Wu J., Galperin M.Y., Koonin E.V., Makarova K.S., Wolf Y.I., Rigden D.J., Dunfield P.F., Wang L., Alam M. Genome Biol. 9:RESEARCH161.1-RESEARCH161.16(2008) [PubMed: 19014707] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000922 Genomic DNA. Translation: ACJ32905.1.
RefSeq	YP_002314890.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	7036778.
GenomeReviews	Gene locus Aflv_0521 in contig CP000922_GR.
KEGG	afl:Aflv_0521.
Organism-specific databases
CMR	Search...
Family and domain databases
HAMAP	MF_00184. [Tree]
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-reg. IPR006195. aa-tRNA-synth_II_cons-reg. IPR004154. Anticodon_bd. IPR012675. b-grasp_ferredoxin-like. IPR004095. TGS. IPR002320. Thr-tRNA-synth_IIa. IPR018158. Thr-tRNA-synth_IIa_cons-reg. IPR012947. tRNA_SAD. [Graphical view]
Gene3D	G3DSA:3.40.50.800. Anticodon_bd. 1 hit. G3DSA:3.10.20.30. Ferredoxin_fold. 1 hit.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF02824. TGS. 1 hit. PF00587. tRNA-synt_2b. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR01047. TRNASYNTHTHR.
TIGRFAMs	TIGR00418. thrS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYT_ANOFW

Accession

Primary (citable) accession number: B7GGN9

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	February 10, 2009
Last modified:	July 28, 2009
This is version 7 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)