Reviewed, UniProtKB/Swiss-Prot B7GGI5 (MRAY_ANOFW)

Names and origin

Protein names

Recommended name:
    Phospho-N-acetylmuramoyl-pentapeptide-transferase
    EC=2.7.8.13
Alternative name(s):
    UDP-MurNAc-pentapeptide phosphotransferase

Gene names

Name:	mraY
Ordered Locus Names:	Aflv_1833

Organism

Anoxybacillus flavithermus (strain DSM 21510 / WK1) [Complete proteome] [HAMAP]

Taxonomic identifier

491915 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Anoxybacillus

Protein attributes

Sequence length	324 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	First step of the lipid cycle reactions in the biosynthesis of the cell wall peptidoglycan By similarity.
Catalytic activity	UDP-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala) + undecaprenyl phosphate = UMP + Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol. HAMAP MF_00038
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00038
Subcellular location	Cell membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the glycosyltransferase 4 family. MraY subfamily.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: HAMAP
Molecular function	phospho-N-acetylmuramoyl-pentapeptide-transferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 324	324	Phospho-N-acetylmuramoyl-pentapeptide-transferase HAMAP MF_00038		PRO_1000116502
Regions
	Transmembrane	5 – 25	21	Potential
	Transmembrane	55 – 75	21	Potential
	Transmembrane	81 – 101	21	Potential
	Transmembrane	122 – 142	21	Potential
	Transmembrane	147 – 167	21	Potential
	Transmembrane	176 – 196	21	Potential
	Transmembrane	203 – 223	21	Potential
	Transmembrane	227 – 247	21	Potential
	Transmembrane	250 – 270	21	Potential
	Transmembrane	302 – 322	21	Potential

Sequences

	Sequence		Length	Mass (Da)
	B7GGI5-1 [UniParc]. Last modified February 10, 2009. Version 1. Checksum: D4DC3F59D318378D	FASTA	324	35,437
	10 20 30 40 50 60 MNEKVILFTA GLAFIITVVL SPIFIPFLRR LKFGQSIREE GPKSHQKKSG TPTMGGLMIL 70 80 90 100 110 120 LSLSITTWLM SDIFFERTAH TYMLLFVTVG YGLLGFIDDF IKVVMKRNLG LTSKQKLAGQ 130 140 150 160 170 180 LLIALIFYFF FQHYSMSTVV SIPGTDVSLD LGVAYVLLII FMLVGGSNAV NLTDGLDGLL 190 200 210 220 230 240 AGTAAIAFGA YAVLAWNQGQ YDVAIFSVAV VGAVLGFLVF NAHPAKVFMG DTGSLALGGA 250 260 270 280 290 300 IVTIAILTKL EILLVIIGGV FVIETLSVII QVISFKTTGK RVFRMSPLHH HYELIGWSEW 310 320 RVVVTFWAVG LLFAILGIYI EVWV

References

[1]

"Encapsulated in silica: genome, proteome and physiology of the thermophilic bacterium Anoxybacillus flavithermus WK1." Saw J.H., Mountain B.W., Feng L., Omelchenko M.V., Hou S., Saito J.A., Stott M.B., Li D., Zhao G., Wu J., Galperin M.Y., Koonin E.V., Makarova K.S., Wolf Y.I., Rigden D.J., Dunfield P.F., Wang L., Alam M. Genome Biol. 9:RESEARCH161.1-RESEARCH161.16(2008) [PubMed: 19014707] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000922 Genomic DNA. Translation: ACJ34194.1.
RefSeq	YP_002316179.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	7038086.
GenomeReviews	Gene locus Aflv_1833 in contig CP000922_GR.
KEGG	afl:Aflv_1833.
Organism-specific databases
CMR	Search...
Family and domain databases
HAMAP	MF_00038. [Tree]
InterPro	IPR000715. Glycosyl_transferase_4. IPR018481. Glycosyl_Trfase_4_cons-reg. IPR003524. PNAcMuramoyl-5peptid_Trfase. IPR018480. PNAcMuramoyl-5peptid_Trfase_CS. [Graphical view]
PANTHER	PTHR22926. Glyco_trans_4. 1 hit. PTHR22926:SF3. PNAcPpept_trans. 1 hit.
Pfam	PF00953. Glycos_transf_4. 1 hit. PF10555. MraY_sig1. 1 hit. [Graphical view]
TIGRFAMs	TIGR00445. mraY. 1 hit.
PROSITE	PS01347. MRAY_1. 1 hit. PS01348. MRAY_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MRAY_ANOFW

Accession

Primary (citable) accession number: B7GGI5

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	February 10, 2009
Last modified:	October 13, 2009
This is version 8 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)