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Protein

Glutamyl-tRNA(Gln) amidotransferase subunit A

Gene

gatA

Organism
Anoxybacillus flavithermus (strain DSM 21510 / WK1)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln).UniRule annotation

Catalytic activityi

ATP + L-glutamyl-tRNA(Gln) + L-glutamine = ADP + phosphate + L-glutaminyl-tRNA(Gln) + L-glutamate.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei79Charge relay systemUniRule annotation1
Active sitei154Charge relay systemUniRule annotation1
Active sitei178Acyl-ester intermediateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamyl-tRNA(Gln) amidotransferase subunit AUniRule annotation (EC:6.3.5.7UniRule annotation)
Short name:
Glu-ADT subunit AUniRule annotation
Gene namesi
Name:gatAUniRule annotation
Ordered Locus Names:Aflv_0259
OrganismiAnoxybacillus flavithermus (strain DSM 21510 / WK1)
Taxonomic identifieri491915 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeAnoxybacillus
Proteomesi
  • UP000000742 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001176021 – 485Glutamyl-tRNA(Gln) amidotransferase subunit AAdd BLAST485

Interactioni

Subunit structurei

Heterotrimer of A, B and C subunits.UniRule annotation

Protein-protein interaction databases

STRINGi491915.Aflv_0259.

Family & Domainsi

Sequence similaritiesi

Belongs to the amidase family. GatA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C3P. Bacteria.
COG0154. LUCA.
HOGENOMiHOG000116699.
KOiK02433.
OMAiKEYFGAG.
OrthoDBiPOG091H00O9.

Family and domain databases

Gene3Di3.90.1300.10. 1 hit.
HAMAPiMF_00120. GatA. 1 hit.
InterProiIPR000120. Amidase.
IPR020556. Amidase_CS.
IPR023631. Amidase_dom.
IPR004412. GatA.
[Graphical view]
PANTHERiPTHR11895. PTHR11895. 1 hit.
PfamiPF01425. Amidase. 1 hit.
[Graphical view]
SUPFAMiSSF75304. SSF75304. 1 hit.
TIGRFAMsiTIGR00132. gatA. 1 hit.
PROSITEiPS00571. AMIDASES. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B7GFW3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLFDYRVSE LHSLLHKKEI SVSDLVDESF KRIHEVDEKV QAFLTLNEEQ
60 70 80 90 100
ARAKAKELDE KLATEKEFGL LFGMPIGIKD NIVTKGLRTT CASKILYNFD
110 120 130 140 150
PIYDATVVER LNEADAVTVG KLNMDEFAMG SSTENSGFQL TRNPWDLERV
160 170 180 190 200
PGGSSGGSAA AVAAGEVPFA LGSDTGGSIR QPAAFCGVVG LKPTYGRVSR
210 220 230 240 250
YGLVAFASSL DQIGPITRTV EDNAYLLQVI SGLDPMDSTS ANVDVPDYVS
260 270 280 290 300
ALTGDIQGLK IAVPKEYLGE GVSEEVRQSV LDALKVLEGL GATWEEVSLP
310 320 330 340 350
HSKYALATYY LLASSEASAN LARFDGVRYG YRTDHAKNLM EMYKQTRSEG
360 370 380 390 400
FGNEVKRRIM LGTFALSSGY YDAYYKKAQK VRTLIKQDFE NVFEKYDVII
410 420 430 440 450
GPTTPTPAFK IGEKTSDPLT MYANDILTIP VNLAGVPGIS IPCGFVNGLP
460 470 480
VGLQIIGKHF DESTIYRVAH AFEQATDYHK QKPTL
Length:485
Mass (Da):53,073
Last modified:February 10, 2009 - v1
Checksum:i3E80448DCF85AA68
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000922 Genomic DNA. Translation: ACJ32643.1.
RefSeqiWP_012573980.1. NC_011567.1.

Genome annotation databases

EnsemblBacteriaiACJ32643; ACJ32643; Aflv_0259.
GeneIDi7036491.
KEGGiafl:Aflv_0259.
PATRICi20952255. VBIAnoFla45531_0264.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000922 Genomic DNA. Translation: ACJ32643.1.
RefSeqiWP_012573980.1. NC_011567.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi491915.Aflv_0259.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACJ32643; ACJ32643; Aflv_0259.
GeneIDi7036491.
KEGGiafl:Aflv_0259.
PATRICi20952255. VBIAnoFla45531_0264.

Phylogenomic databases

eggNOGiENOG4105C3P. Bacteria.
COG0154. LUCA.
HOGENOMiHOG000116699.
KOiK02433.
OMAiKEYFGAG.
OrthoDBiPOG091H00O9.

Family and domain databases

Gene3Di3.90.1300.10. 1 hit.
HAMAPiMF_00120. GatA. 1 hit.
InterProiIPR000120. Amidase.
IPR020556. Amidase_CS.
IPR023631. Amidase_dom.
IPR004412. GatA.
[Graphical view]
PANTHERiPTHR11895. PTHR11895. 1 hit.
PfamiPF01425. Amidase. 1 hit.
[Graphical view]
SUPFAMiSSF75304. SSF75304. 1 hit.
TIGRFAMsiTIGR00132. gatA. 1 hit.
PROSITEiPS00571. AMIDASES. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGATA_ANOFW
AccessioniPrimary (citable) accession number: B7GFW3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: February 10, 2009
Last modified: November 2, 2016
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.