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Protein

Glutamyl-tRNA(Gln) amidotransferase subunit A

Gene

gatA

Organism
Anoxybacillus flavithermus (strain DSM 21510 / WK1)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln).UniRule annotation

Catalytic activityi

ATP + L-glutamyl-tRNA(Gln) + L-glutamine = ADP + phosphate + L-glutaminyl-tRNA(Gln) + L-glutamate.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei79Charge relay systemUniRule annotation1
Active sitei154Charge relay systemUniRule annotation1
Active sitei178Acyl-ester intermediateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciAFLA491915:G1GAW-313-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamyl-tRNA(Gln) amidotransferase subunit AUniRule annotation (EC:6.3.5.7UniRule annotation)
Short name:
Glu-ADT subunit AUniRule annotation
Gene namesi
Name:gatAUniRule annotation
Ordered Locus Names:Aflv_0259
OrganismiAnoxybacillus flavithermus (strain DSM 21510 / WK1)
Taxonomic identifieri491915 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeAnoxybacillus
Proteomesi
  • UP000000742 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001176021 – 485Glutamyl-tRNA(Gln) amidotransferase subunit AAdd BLAST485

Proteomic databases

PRIDEiB7GFW3

Interactioni

Subunit structurei

Heterotrimer of A, B and C subunits.UniRule annotation

Protein-protein interaction databases

STRINGi491915.Aflv_0259

Structurei

3D structure databases

SMRiB7GFW3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the amidase family. GatA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C3P Bacteria
COG0154 LUCA
HOGENOMiHOG000116699
KOiK02433
OMAiPITQNVE
OrthoDBiPOG091H00O9

Family and domain databases

Gene3Di3.90.1300.10, 1 hit
HAMAPiMF_00120 GatA, 1 hit
InterProiView protein in InterPro
IPR000120 Amidase
IPR020556 Amidase_CS
IPR023631 Amidase_dom
IPR036928 AS_sf
IPR004412 GatA
PANTHERiPTHR11895 PTHR11895, 1 hit
PfamiView protein in Pfam
PF01425 Amidase, 1 hit
SUPFAMiSSF75304 SSF75304, 1 hit
TIGRFAMsiTIGR00132 gatA, 1 hit
PROSITEiView protein in PROSITE
PS00571 AMIDASES, 1 hit

Sequencei

Sequence statusi: Complete.

B7GFW3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLFDYRVSE LHSLLHKKEI SVSDLVDESF KRIHEVDEKV QAFLTLNEEQ
60 70 80 90 100
ARAKAKELDE KLATEKEFGL LFGMPIGIKD NIVTKGLRTT CASKILYNFD
110 120 130 140 150
PIYDATVVER LNEADAVTVG KLNMDEFAMG SSTENSGFQL TRNPWDLERV
160 170 180 190 200
PGGSSGGSAA AVAAGEVPFA LGSDTGGSIR QPAAFCGVVG LKPTYGRVSR
210 220 230 240 250
YGLVAFASSL DQIGPITRTV EDNAYLLQVI SGLDPMDSTS ANVDVPDYVS
260 270 280 290 300
ALTGDIQGLK IAVPKEYLGE GVSEEVRQSV LDALKVLEGL GATWEEVSLP
310 320 330 340 350
HSKYALATYY LLASSEASAN LARFDGVRYG YRTDHAKNLM EMYKQTRSEG
360 370 380 390 400
FGNEVKRRIM LGTFALSSGY YDAYYKKAQK VRTLIKQDFE NVFEKYDVII
410 420 430 440 450
GPTTPTPAFK IGEKTSDPLT MYANDILTIP VNLAGVPGIS IPCGFVNGLP
460 470 480
VGLQIIGKHF DESTIYRVAH AFEQATDYHK QKPTL
Length:485
Mass (Da):53,073
Last modified:February 10, 2009 - v1
Checksum:i3E80448DCF85AA68
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000922 Genomic DNA Translation: ACJ32643.1
RefSeqiWP_012573980.1, NC_011567.1

Genome annotation databases

EnsemblBacteriaiACJ32643; ACJ32643; Aflv_0259
KEGGiafl:Aflv_0259
PATRICifig|491915.6.peg.264

Similar proteinsi

Entry informationi

Entry nameiGATA_ANOFW
AccessioniPrimary (citable) accession number: B7GFW3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: February 10, 2009
Last modified: May 23, 2018
This is version 60 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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