B7GFV3 (ROCA_ANOFW) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
1-pyrroline-5-carboxylate dehydrogenase
Short name=P5C dehydrogenase
EC=1.5.1.12

Gene names

Name:	rocA
Ordered Locus Names:	Aflv_0249

Organism

Anoxybacillus flavithermus (strain DSM 21510 / WK1) [Complete proteome] [HAMAP]

Taxonomic identifier

491915 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Anoxybacillus

Protein attributes

Sequence length	515 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	(S)-1-pyrroline-5-carboxylate + NAD(P)+ + 2 H2O = L-glutamate + NAD(P)H. HAMAP MF_00733
Pathway	Amino-acid degradation; L-proline degradation into L-glutamate; L-glutamate from L-proline: step 2/2. HAMAP MF_00733
Sequence similarities	Belongs to the aldehyde dehydrogenase family. RocA subfamily.

Ontologies

Keywords
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proline biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	1-pyrroline-5-carboxylate dehydrogenase activity Inferred from electronic annotation. Source: EC oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 515	515	1-pyrroline-5-carboxylate dehydrogenase HAMAP MF_00733		PRO_1000132748
Sites
	Active site	286	1	By similarity
	Active site	320	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	B7GFV3 [UniParc]. Last modified February 10, 2009. Version 1. Checksum: 740FDEADC2C72C0D	FASTA	515	56,735
	10 20 30 40 50 60 MVQPYKHEPF TDFTVEANKK AFEEGLKTVQ AYLGQDYPLV IGGERVMTED KIVSINPANK 70 80 90 100 110 120 TEVVGRVAKA NKDLAEKAMQ TADAAFKWWS KTKPEMRADI LFRAAAIVRR RKHEFSALLV 130 140 150 160 170 180 KEAGKPWKEA DADTAEAIDF MEYYARQMLK LKDGIPVESR PGETNRFFYI PLGVGVVISP 190 200 210 220 230 240 WNFPFAIMAG TTVAALVTGN TVLLKPASAT PVVAYKFVEV LEEAGLPAGV LNYIPGSGAE 250 260 270 280 290 300 VGDYLVDHPR TRFISFTGSR DVGIRIYERA AKVHPGQIWL KRVIAEMGGK DTIVVDKEAD 310 320 330 340 350 360 LELAAQSIVA SAFGFSGQKC SACSRVVALE DVYDQVLNRV VELTKQLKVG NPEEQSTFMG 370 380 390 400 410 420 PVIDQSAYNK IMEYIEIGKQ EGKLMTGGEG DDSKGFFIQP TVFADLDPKA RIMQEEIFGP 430 440 450 460 470 480 VVAFTKAKDF DHALEIANNT EYGLTGAVIS NNRFNLEKAR EEFHVGNLYF NRGCTGAIVG 490 500 510 YHPFGGFNMS GTDSKAGGPD YLLLHMQAKT VSEMF

References

[1]

"Encapsulated in silica: genome, proteome and physiology of the thermophilic bacterium Anoxybacillus flavithermus WK1." Saw J.H., Mountain B.W., Feng L., Omelchenko M.V., Hou S., Saito J.A., Stott M.B., Li D., Zhao G., Wu J., Galperin M.Y., Koonin E.V., Makarova K.S., Wolf Y.I., Rigden D.J., Dunfield P.F., Wang L., Alam M. Genome Biol. 9:R161.1-R161.16(2008) [PubMed: 19014707] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 21510 / WK1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000922 Genomic DNA. Translation: ACJ32633.1.
RefSeq	YP_002314618.1. NC_011567.1.
3D structure databases
ProteinModelPortal	B7GFV3.
ModBase	Search...
Protein-protein interaction databases
STRING	B7GFV3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7036481.
GenomeReviews	Gene locus Aflv_0249 in contig CP000922_GR.
KEGG	afl:Aflv_0249.
PATRIC	20952235. VBIAnoFla45531_0254.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG752218.
ProtClustDB	PRK03137.
Family and domain databases
HAMAP	MF_00733. RocA. [Tree]
InterPro	IPR016161. Ald_DH/histidinol_DH. IPR016163. Ald_DH_C. IPR016160. Ald_DH_CS. IPR016162. Ald_DH_N. IPR015590. Aldehyde_DH_dom. IPR005932. Delta1-pyrroline-5-COlate_DH-2. [Graphical view]
Gene3D	G3DSA:3.40.309.10. Aldehyde_dehydrogenase_C. 1 hit. G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
KO	K00294.
Pfam	PF00171. Aldedh. 1 hit. [Graphical view]
SUPFAM	SSF53720. Aldehyde_DH/Histidinol_DH. 1 hit.
TIGRFAMs	TIGR01237. D1pyr5carbox2. 1 hit.
PROSITE	PS00070. ALDEHYDE_DEHYDR_CYS. 1 hit. PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ROCA_ANOFW

Accession

Primary (citable) accession number: B7GFV3

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	February 10, 2009
Last modified:	January 25, 2012
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program