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Protein

Bifunctional purine biosynthesis protein PurH

Gene

purH

Organism
Anoxybacillus flavithermus (strain DSM 21510 / WK1)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

10-formyltetrahydrofolate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide = tetrahydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide.UniRule annotation
IMP + H2O = 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide.UniRule annotation

Pathwayi

GO - Molecular functioni

  1. IMP cyclohydrolase activity Source: UniProtKB-HAMAP
  2. phosphoribosylaminoimidazolecarboxamide formyltransferase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. 'de novo' IMP biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Transferase

Keywords - Biological processi

Purine biosynthesis

Enzyme and pathway databases

BioCyciAFLA491915:GHEO-288-MONOMER.
UniPathwayiUPA00074; UER00133.
UPA00074; UER00135.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional purine biosynthesis protein PurHUniRule annotation
Including the following 2 domains:
Phosphoribosylaminoimidazolecarboxamide formyltransferaseUniRule annotation (EC:2.1.2.3UniRule annotation)
Alternative name(s):
AICAR transformylaseUniRule annotation
IMP cyclohydrolaseUniRule annotation (EC:3.5.4.10UniRule annotation)
Alternative name(s):
ATICUniRule annotation
IMP synthaseUniRule annotation
InosinicaseUniRule annotation
Gene namesi
Name:purHUniRule annotation
Ordered Locus Names:Aflv_0238
OrganismiAnoxybacillus flavithermus (strain DSM 21510 / WK1)
Taxonomic identifieri491915 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeAnoxybacillus
ProteomesiUP000000742: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 511511Bifunctional purine biosynthesis protein PurHPRO_1000117863Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi491915.Aflv_0238.

Structurei

3D structure databases

ProteinModelPortaliB7GFU2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

The IMP cyclohydrolase activity resides in the N-terminal region.UniRule annotation

Sequence similaritiesi

Belongs to the PurH family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0138.
HOGENOMiHOG000230373.
KOiK00602.
OMAiPCGVAEG.
OrthoDBiEOG6QCDFF.

Family and domain databases

Gene3Di3.40.140.20. 2 hits.
3.40.50.1380. 1 hit.
HAMAPiMF_00139. PurH.
InterProiIPR024051. AICAR_Tfase_dom.
IPR002695. AICARFT_IMPCHas.
IPR016193. Cytidine_deaminase-like.
IPR011607. MGS-like_dom.
[Graphical view]
PANTHERiPTHR11692. PTHR11692. 1 hit.
PfamiPF01808. AICARFT_IMPCHas. 1 hit.
PF02142. MGS. 1 hit.
[Graphical view]
PIRSFiPIRSF000414. AICARFT_IMPCHas. 1 hit.
SMARTiSM00798. AICARFT_IMPCHas. 1 hit.
SM00851. MGS. 1 hit.
[Graphical view]
SUPFAMiSSF52335. SSF52335. 1 hit.
SSF53927. SSF53927. 1 hit.
TIGRFAMsiTIGR00355. purH. 1 hit.

Sequencei

Sequence statusi: Complete.

B7GFU2-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKRRAIISVS NKKGIVTFAK QLAELGVEII STGGTKRVLA EHGVPVISIS
60 70 80 90 100
DVTNFPEILD GRVKTLHPNI HGGLLAVRDD ETHQQQLQAH NITPIDFVVV
110 120 130 140 150
NLYPFQETIA KPNVTLMEAI EQIDIGGPTM LRAAAKNHAY VTAVVDPADY
160 170 180 190 200
HLVIEQLKQY GEVLLETRRA LAAKVFRHTA AYDAMIAQYL TNIVGETYPE
210 220 230 240 250
TMTMTFVKKQ SLRYGENPHQ TAAFYEKPLS SPFSIAAAKQ LHGKELSYNN
260 270 280 290 300
INDANAALQI VAEFVEPAAV AVKHMNPCGV GVGETIAEAF QKAYEADPTS
310 320 330 340 350
IFGGIVALNR EVDEQMAAKL HDIFLEIVIA PSFTEQALEI LTRKKNIRLL
360 370 380 390 400
TVDFEVERKK EPFVVSVRGG LLVQDEDTYT IDDATLRVVT ERKPTEEEWS
410 420 430 440 450
NLTFAWRVVK HVKSNAIVLA KNGMTIGVGA GQMNRVGAAK IAIEQAGDRA
460 470 480 490 500
KGAVLASDAF FPMSDTVEAA AQAGVTAIIQ PGGSIRDEDS IQKANEYGIA
510
MVFTGVRHFK H
Length:511
Mass (Da):55,923
Last modified:February 10, 2009 - v1
Checksum:i8142E20B885C4433
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000922 Genomic DNA. Translation: ACJ32622.1.
RefSeqiWP_012573961.1. NC_011567.1.
YP_002314607.1. NC_011567.1.

Genome annotation databases

EnsemblBacteriaiACJ32622; ACJ32622; Aflv_0238.
GeneIDi7036470.
KEGGiafl:Aflv_0238.
PATRICi20952215. VBIAnoFla45531_0244.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000922 Genomic DNA. Translation: ACJ32622.1.
RefSeqiWP_012573961.1. NC_011567.1.
YP_002314607.1. NC_011567.1.

3D structure databases

ProteinModelPortaliB7GFU2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi491915.Aflv_0238.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACJ32622; ACJ32622; Aflv_0238.
GeneIDi7036470.
KEGGiafl:Aflv_0238.
PATRICi20952215. VBIAnoFla45531_0244.

Phylogenomic databases

eggNOGiCOG0138.
HOGENOMiHOG000230373.
KOiK00602.
OMAiPCGVAEG.
OrthoDBiEOG6QCDFF.

Enzyme and pathway databases

UniPathwayiUPA00074; UER00133.
UPA00074; UER00135.
BioCyciAFLA491915:GHEO-288-MONOMER.

Family and domain databases

Gene3Di3.40.140.20. 2 hits.
3.40.50.1380. 1 hit.
HAMAPiMF_00139. PurH.
InterProiIPR024051. AICAR_Tfase_dom.
IPR002695. AICARFT_IMPCHas.
IPR016193. Cytidine_deaminase-like.
IPR011607. MGS-like_dom.
[Graphical view]
PANTHERiPTHR11692. PTHR11692. 1 hit.
PfamiPF01808. AICARFT_IMPCHas. 1 hit.
PF02142. MGS. 1 hit.
[Graphical view]
PIRSFiPIRSF000414. AICARFT_IMPCHas. 1 hit.
SMARTiSM00798. AICARFT_IMPCHas. 1 hit.
SM00851. MGS. 1 hit.
[Graphical view]
SUPFAMiSSF52335. SSF52335. 1 hit.
SSF53927. SSF53927. 1 hit.
TIGRFAMsiTIGR00355. purH. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Encapsulated in silica: genome, proteome and physiology of the thermophilic bacterium Anoxybacillus flavithermus WK1."
    Saw J.H., Mountain B.W., Feng L., Omelchenko M.V., Hou S., Saito J.A., Stott M.B., Li D., Zhao G., Wu J., Galperin M.Y., Koonin E.V., Makarova K.S., Wolf Y.I., Rigden D.J., Dunfield P.F., Wang L., Alam M.
    Genome Biol. 9:R161.1-R161.16(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 21510 / WK1.

Entry informationi

Entry nameiPUR9_ANOFW
AccessioniPrimary (citable) accession number: B7GFU2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: February 10, 2009
Last modified: February 4, 2015
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.