Reviewed, UniProtKB/Swiss-Prot B7GFT8 (PURL_ANOFW)

Names and origin

Protein names

Recommended name:
    Phosphoribosylformylglycinamidine synthase 2
    EC=6.3.5.3
Alternative name(s):
    Phosphoribosylformylglycinamidine synthase II
      Short name=FGAM synthase II

Gene names

Name:	purL
Ordered Locus Names:	Aflv_0234

Organism

Anoxybacillus flavithermus (strain DSM 21510 / WK1) [Complete proteome] [HAMAP]

Taxonomic identifier

491915 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Anoxybacillus

Protein attributes

Sequence length	740 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate. HAMAP MF_00420
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2. HAMAP MF_00420
Subunit structure	Heterodimer of two subunits, purQ and purL By similarity. HAMAP MF_00420
Subcellular location	Cytoplasm By similarity HAMAP MF_00420.
Sequence similarities	Belongs to the FGAMS family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP phosphoribosylformylglycinamidine synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 740	740	Phosphoribosylformylglycinamidine synthase 2 HAMAP MF_00420		PRO_1000194817
Regions
	Nucleotide binding	111 – 122	12	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	B7GFT8-1 [UniParc]. Last modified February 10, 2009. Version 1. Checksum: B93BC1EFC06B3D38	FASTA	740	80,716
	10 20 30 40 50 60 MSLLLEPSPT MIKEQKMYRD MGLTDEEFAM IERILGRLPN YTETGIFSVM WSEHCSYKNS 70 80 90 100 110 120 KPVLKKFPTE GKHVLQGPGE GAGIVDIGDG LAVAFKIESH NHPSAIEPYQ GAATGVGGII 130 140 150 160 170 180 RDVFSMGARP IALLNSLRFG ELTSPRVKYL FERVVAGIAG YGNCVGIPTV GGEVQFDAAY 190 200 210 220 230 240 EGNPLVNAMC VGVIRHEDIQ KGIASGVGNT VMYVGAKTGR DGIHGATFAS EELTEQSEQK 250 260 270 280 290 300 RPAVQVGDPF MEKLLLEACL EVIHCDALVG MQDMGAAGLT SSSAEMASKA GSGIELNLDL 310 320 330 340 350 360 VPQRETGMTP YEMMLSESQE RMLLVVKKGR EQEIMDVFSK YGLEAKAIGR VTDDHMLRLY 370 380 390 400 410 420 HRGEVVAEIP VDALAKDAPV YHKPSQEPAY YREFQTMEYV PTVDNYEETL LALLSQPTIA 430 440 450 460 470 480 SKEWVYEQYD YMVRTNTVVA PGSDAAVLRI RGTNKALAMT TDCNSRYVYL DPEVGGKIAI 490 500 510 520 530 540 AEAARNIVCS GAQPLAVTDC LNFGNPEKPE IFWQLEKAVD GMSEACRILE TPVISGNVSL 550 560 570 580 590 600 YNETNGEAIY PTPIVGMVGI VEHVDHITTQ AFKQPGDLIY IIGEAKQEFG GSELQKWLTG 610 620 630 640 650 660 RIFGKAPTID LHVEVKRQQQ LLTAIRAGVV ASAHDVSEGG LAVALAECVM SAQGLGARVE 670 680 690 700 710 720 MKGDVVAELF SETQSRFIVS VKKEHQQMFE RLVQAVRIGE VTNDGTLHVT AEDTCILHVP 730 740 VETMRNVWKG AIPCLLKSKD

References

[1]

"Encapsulated in silica: genome, proteome and physiology of the thermophilic bacterium Anoxybacillus flavithermus WK1." Saw J.H., Mountain B.W., Feng L., Omelchenko M.V., Hou S., Saito J.A., Stott M.B., Li D., Zhao G., Wu J., Galperin M.Y., Koonin E.V., Makarova K.S., Wolf Y.I., Rigden D.J., Dunfield P.F., Wang L., Alam M. Genome Biol. 9:R161.1-R161.16(2008) [PubMed: 19014707] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000922 Genomic DNA. Translation: ACJ32618.1.
RefSeq	YP_002314603.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	7036466.
GenomeReviews	Gene locus Aflv_0234 in contig CP000922_GR.
KEGG	afl:Aflv_0234.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG311214.
Family and domain databases
HAMAP	MF_00420. PurL_2. [Tree]
InterPro	IPR000728. AIR_synth. IPR010918. AIR_synth_C. IPR010074. PRibForGlyAmidine_synth_II. IPR016188. PurM_N-like. [Graphical view]
Pfam	PF00586. AIRS. 2 hits. PF02769. AIRS_C. 2 hits. [Graphical view]
TIGRFAMs	TIGR01736. FGAM_synth_II. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PURL_ANOFW

Accession

Primary (citable) accession number: B7GFT8

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	February 10, 2009
Last modified:	February 9, 2010
This is version 12 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)