Reviewed, UniProtKB/Swiss-Prot B7GFR1 (SYD_ANOFW)

Names and origin

Protein names

Recommended name:
    Aspartyl-tRNA synthetase
    EC=6.1.1.12
Alternative name(s):
    Aspartate--tRNA ligase
      Short name=AspRS

Gene names

Name:	aspS
Ordered Locus Names:	Aflv_0731

Organism

Anoxybacillus flavithermus (strain DSM 21510 / WK1) [Complete proteome] [HAMAP]

Taxonomic identifier

491915 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Anoxybacillus

Protein attributes

Sequence length	592 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-aspartate + tRNA(Asp) = AMP + diphosphate + L-aspartyl-tRNA(Asp). HAMAP MF_00044
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aspartyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP aspartate-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 592	592	Aspartyl-tRNA synthetase HAMAP MF_00044		PRO_1000198952

Sequences

	Sequence		Length	Mass (Da)
	B7GFR1-1 [UniParc]. Last modified February 10, 2009. Version 1. Checksum: C05F3F63BE98DE77	FASTA	592	67,044
	10 20 30 40 50 60 MFGRTHYCGE VTDMAIGQTV RLKGWVQKRR DLGGLIFIDL RDRTGIVQIV FSPDVSKEAL 70 80 90 100 110 120 QVAETIRSEY VLDVEGTVVQ REEGQVNPNL PTGTIEVHAM HVTILSEAKT PPFLISDKTD 130 140 150 160 170 180 VSEDVRLKYR YLDLRRPVMF QTFKMRHQVT KAIRDFLDEE GFLEVETPIL TKSTPEGARD 190 200 210 220 230 240 YLVPSRVHPG EFYALPQSPQ IFKQLLMVAG FERYYQIARC FRDEDLRADR QPEFTQVDIE 250 260 270 280 290 300 TSFMSQDDIL AMIERMMARV MKVAKGVDIS IPFPRMSYDE AIARYGSDKP DTRFGLELVD 310 320 330 340 350 360 LSEQVKDCGF KVFASAVQNG GQVKAINVKG AADKYSRKDI DALTEYVARY GAKGLAWLKV 370 380 390 400 410 420 EADGLKGPIA KFFTEDEQKQ MMQTLEAETG DLLLFVADKK SVVADALGAL RLKLGKDLQL 430 440 450 460 470 480 IDESAFHFLW ITDWPLFEYD EEEGRYYAAH HPFTMPVRED VPKFETDPAS VRAQAYDLVL 490 500 510 520 530 540 NGYELGGGSL RIFERDIQEK MFKTLGFTEE QAREQFGFLL EAFEYGTPPH GGIALGLDRL 550 560 570 580 590 VMLLAGRSNL RDTIAFPKTA SASCLLTEAP SAVSEEQLEE LHLRIKDVQK VD

References

[1]

"Encapsulated in silica: genome, proteome and physiology of the thermophilic bacterium Anoxybacillus flavithermus WK1." Saw J.H., Mountain B.W., Feng L., Omelchenko M.V., Hou S., Saito J.A., Stott M.B., Li D., Zhao G., Wu J., Galperin M.Y., Koonin E.V., Makarova K.S., Wolf Y.I., Rigden D.J., Dunfield P.F., Wang L., Alam M. Genome Biol. 9:RESEARCH161.1-RESEARCH161.16(2008) [PubMed: 19014707] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000922 Genomic DNA. Translation: ACJ33110.1.
RefSeq	YP_002315095.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	7036988.
GenomeReviews	Gene locus Aflv_0731 in contig CP000922_GR.
KEGG	afl:Aflv_0731.
Organism-specific databases
CMR	Search...
Family and domain databases
HAMAP	MF_00044. [Tree]
InterPro	IPR004364. aa-tRNA-synt_II. IPR018150. aa-tRNA-synt_II-like. IPR006195. aa-tRNA-synth_II_cons-reg. IPR002312. Asp-tRNA-synth_IIb. IPR020564. Asp-tRNA-synth_IIb_bac-type. IPR004524. Asp-tRNA-synth_IIb_bac/mt. IPR018153. Asp-tRNA-synth_IIb_C_bac/mt. IPR004115. GAD_dom. IPR012340. NA-bd_OB-fold. IPR004365. NA_bd_OB_tRNA-helicase. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
PANTHER	PTHR22594. aa-tRNA-synt_II. 1 hit. PTHR22594:SF5. AspS_bac. 1 hit.
Pfam	PF02938. GAD. 1 hit. PF00152. tRNA-synt_2. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
PRINTS	PR01042. TRNASYNTHASP.
TIGRFAMs	TIGR00459. aspS_bact. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYD_ANOFW

Accession

Primary (citable) accession number: B7GFR1

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	February 10, 2009
Last modified:	September 22, 2009
This is version 8 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)