Reviewed, UniProtKB/Swiss-Prot B7GFQ4 (GCP_ANOFW)

Names and origin

Protein names

Recommended name:
    Probable O-sialoglycoprotein endopeptidase
      Short name=Glycoprotease
    EC=3.4.24.57

Gene names

Name:	gcp
Ordered Locus Names:	Aflv_0206

Organism

Anoxybacillus flavithermus (strain DSM 21510 / WK1) [Complete proteome] [HAMAP]

Taxonomic identifier

491915 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Anoxybacillus

Protein attributes

Sequence length	341 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Hydrolysis of O-sialoglycoproteins; cleaves 31-Arg-|-Asp-32 bond in glycophorin A. Does not cleave unglycosylated proteins, desialylated glycoproteins or glycoproteins that are only N-glycosylated. HAMAP MF_01445
Cofactor	Zinc By similarity. HAMAP MF_01445
Sequence similarities	Belongs to the peptidase M22 family.

Ontologies

Keywords
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Metalloprotease Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Molecular function	metalloendopeptidase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 341	341	Probable O-sialoglycoprotein endopeptidase HAMAP MF_01445		PRO_1000145947
Sites
	Metal binding	120	1	Zinc Potential
	Metal binding	124	1	Zinc Potential

Sequences

	Sequence		Length	Mass (Da)
	B7GFQ4-1 [UniParc]. Last modified February 10, 2009. Version 1. Checksum: 3BE83C1F6C5867F8	FASTA	341	36,840
	10 20 30 40 50 60 MGEFMNKKDM FVLGIETSCD ETAAAIVKNG REIVANVVAS QMESHQRFGG VVPEIASRHH 70 80 90 100 110 120 VEQITLVLEE TMKQANMDIS QLDAIAVTEG PGLVGALLIG VNAAKALAFA HRIPLVGVHH 130 140 150 160 170 180 IAGHIYANQL VQEMKFPLLA LVVSGGHTEL IYMKEHGIFE VIGETRDDAA GEAYDKVARA 190 200 210 220 230 240 LQLPYPGGPH IDRLAKQGKP IIDLPRAWLE EGSYDFSFSG LKSAVINTLH NAKQRGEMIR 250 260 270 280 290 300 PEDMAASFQA SVVEVLVRKT IEAAMHYSVK QILLAGGVAA NEGLRTQLQK QMDQLPEVEL 310 320 330 340 VIPPLSLCTD NAAMIAAAGT VLFQQGKRAT MALNANPSLL L

References

[1]

"Encapsulated in silica: genome, proteome and physiology of the thermophilic bacterium Anoxybacillus flavithermus WK1." Saw J.H., Mountain B.W., Feng L., Omelchenko M.V., Hou S., Saito J.A., Stott M.B., Li D., Zhao G., Wu J., Galperin M.Y., Koonin E.V., Makarova K.S., Wolf Y.I., Rigden D.J., Dunfield P.F., Wang L., Alam M. Genome Biol. 9:R161.1-R161.16(2008) [PubMed: 19014707] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000922 Genomic DNA. Translation: ACJ32590.1.
RefSeq	YP_002314575.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	7036435.
GenomeReviews	Gene locus Aflv_0206 in contig CP000922_GR.
KEGG	afl:Aflv_0206.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG304663.
Family and domain databases
HAMAP	MF_01445. Glycoptase_bact. [Tree]
InterPro	IPR009180. Pept_M22_O-sialoglycoprot. IPR000905. Peptidase_M22. IPR017860. Peptidase_M22_CS. IPR017861. Peptidase_M22_subgr. [Graphical view]
PANTHER	PTHR11735. Pept_M22_Osialgl. 1 hit.
Pfam	PF00814. Peptidase_M22. 1 hit. [Graphical view]
PRINTS	PR00789. OSIALOPTASE.
TIGRFAMs	TIGR00329. gcp. 1 hit.
PROSITE	PS01016. GLYCOPROTEASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GCP_ANOFW

Accession

Primary (citable) accession number: B7GFQ4

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	February 10, 2009
Last modified:	February 9, 2010
This is version 10 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)