B7GFP5 (ALR_ANOFW) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alanine racemase
EC=5.1.1.1

Gene names

Name:	alr
Ordered Locus Names:	Aflv_0197

Organism

Anoxybacillus flavithermus (strain DSM 21510 / WK1) [Complete proteome] [HAMAP]

Taxonomic identifier

491915 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Anoxybacillus

Protein attributes

Sequence length	385 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Provides the D-alanine required for cell wall biosynthesis By similarity. HAMAP MF_01201
Catalytic activity	L-alanine = D-alanine. HAMAP MF_01201
Cofactor	Pyridoxal phosphate By similarity. HAMAP MF_01201
Pathway	Amino-acid biosynthesis; D-alanine biosynthesis; D-alanine from L-alanine: step 1/1. HAMAP MF_01201 Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_01201
Sequence similarities	Belongs to the alanine racemase family.

Ontologies

Keywords
Biological process	Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Ligand	Pyridoxal phosphate
Molecular function	Isomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanine metabolic process Inferred from electronic annotation. Source: InterPro cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	alanine racemase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 385	385	Alanine racemase HAMAP MF_01201		PRO_1000138579
Sites
	Active site	40	1	Proton acceptor; specific for D-alanine By similarity
	Active site	268	1	Proton acceptor; specific for L-alanine By similarity
Amino acid modifications
	Modified residue	40	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	B7GFP5 [UniParc]. Last modified February 10, 2009. Version 1. Checksum: 31A13949F934A7A1	FASTA	385	43,245
	10 20 30 40 50 60 MEKAFYRDTW AEIDLDAIFY NVQSMKQHVG SHVEVIAVVK ANAYGHGDIQ VAKTALEAGA 70 80 90 100 110 120 TRLAVAFLDE ALALRKKGIS LDVPILVLGA TKPQYAPLAA SQNIALTVFR ADWFEQAIVY 130 140 150 160 170 180 APYEQPLNVH MKLDTGMGRL GAKTKEEVQQ MIYAIEQHPS FILEGVYTHF ATADEQNIDY 190 200 210 220 230 240 FSFQYDTFLH MLEWLSIQPP LIHCANSAAG LRYPDRIFNA VRFGISMYGL APSQEMKSLL 250 260 270 280 290 300 PYPLKEAFSL HSRLTHVKKV KGGEKISYGA TYEAETDEWI GTVPIGYADG WLRRMQHFSV 310 320 330 340 350 360 LVDGKRAPIV GRVCMDQMMI RLPYELPVGT KVTLIGEQQG DRISVDDVAA HVNTINYEIP 370 380 CTISYRVPRI FLKNKSIIEV RNAVL

References

[1]

"Encapsulated in silica: genome, proteome and physiology of the thermophilic bacterium Anoxybacillus flavithermus WK1." Saw J.H., Mountain B.W., Feng L., Omelchenko M.V., Hou S., Saito J.A., Stott M.B., Li D., Zhao G., Wu J., Galperin M.Y., Koonin E.V., Makarova K.S., Wolf Y.I., Rigden D.J., Dunfield P.F., Wang L., Alam M. Genome Biol. 9:R161.1-R161.16(2008) [PubMed: 19014707] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 21510 / WK1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000922 Genomic DNA. Translation: ACJ32581.1.
RefSeq	YP_002314566.1. NC_011567.1.
3D structure databases
ProteinModelPortal	B7GFP5.
ModBase	Search...
Protein-protein interaction databases
STRING	B7GFP5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7036410.
GenomeReviews	Gene locus Aflv_0197 in contig CP000922_GR.
KEGG	afl:Aflv_0197.
PATRIC	20952089. VBIAnoFla45531_0200.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG712172.
ProtClustDB	CLSK712473.
Family and domain databases
HAMAP	MF_01201. Ala_racemase. [Tree]
InterPro	IPR000821. Ala_racemase. IPR009006. Ala_racemase/Decarboxylase_C. IPR011079. Ala_racemase_C. IPR001608. Ala_racemase_N. IPR020622. Ala_racemase_pyridoxalP-BS. [Graphical view]
Gene3D	G3DSA:2.40.37.10. Ala_racemase/Decarboxylase_C. 1 hit.
KO	K01775.
Pfam	PF00842. Ala_racemase_C. 1 hit. PF01168. Ala_racemase_N. 1 hit. [Graphical view]
PRINTS	PR00992. ALARACEMASE.
SMART	SM01005. Ala_racemase_C. 1 hit. [Graphical view]
SUPFAM	SSF50621. Racem_decarbox_C. 1 hit.
TIGRFAMs	TIGR00492. Alr. 1 hit.
PROSITE	PS00395. ALANINE_RACEMASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALR_ANOFW

Accession

Primary (citable) accession number: B7GFP5

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	February 10, 2009
Last modified:	January 25, 2012
This is version 19 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program