B7GFB0 (LSPA_ANOFW) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Lipoprotein signal peptidase
EC=3.4.23.36

Alternative name(s):
Prolipoprotein signal peptidase
Signal peptidase II
Short name=SPase II

Gene names

Name:	lspA
Ordered Locus Names:	Aflv_1810

Organism

Anoxybacillus flavithermus (strain DSM 21510 / WK1) [Complete proteome] [HAMAP]

Taxonomic identifier

491915 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Anoxybacillus

Protein attributes

Sequence length	168 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This protein specifically catalyzes the removal of signal peptides from prolipoproteins By similarity. HAMAP MF_00161
Catalytic activity	Release of signal peptides from bacterial membrane prolipoproteins. Hydrolyzes -Xaa-Yaa-Zaa-|-(S,diacylglyceryl)Cys-, in which Xaa is hydrophobic (preferably Leu), and Yaa (Ala or Ser) and Zaa (Gly or Ala) have small, neutral side chains. HAMAP MF_00161
Pathway	Protein modification; lipoprotein biosynthesis (signal peptide cleavage). HAMAP MF_00161
Subcellular location	Cell membrane; Multi-pass membrane protein By similarity HAMAP MF_00161.
Sequence similarities	Belongs to the peptidase A8 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Aspartyl protease Hydrolase Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aspartic-type endopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 168	168	Lipoprotein signal peptidase HAMAP MF_00161		PRO_1000190786
Regions
	Transmembrane	8 – 28	21	Helical; Potential
	Transmembrane	61 – 81	21	Helical; Potential
	Transmembrane	91 – 111	21	Helical; Potential
	Transmembrane	128 – 148	21	Helical; Potential
Sites
	Active site	108	1	By similarity
	Active site	135	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	B7GFB0 [UniParc]. Last modified February 10, 2009. Version 1. Checksum: C6F9B3E3C5D4E66C	FASTA	168	19,455
	10 20 30 40 50 60 MLWRGVMLYY LLAFVVILID QWTKWLVVRY MELGESIPII ENVLYMTSHR NRGAAWGMLQ 70 80 90 100 110 120 GQFWLFYLIT IVVVVGIVIY IQRLQPTQRL FGIALGLMLG GALGNFIDRI FRKEVVDFVH 130 140 150 160 TYIFNYSFPI FNVADAALTI GVALMFIYTW TEEKQRKGMS DGANSTHD

References

[1]

"Encapsulated in silica: genome, proteome and physiology of the thermophilic bacterium Anoxybacillus flavithermus WK1." Saw J.H., Mountain B.W., Feng L., Omelchenko M.V., Hou S., Saito J.A., Stott M.B., Li D., Zhao G., Wu J., Galperin M.Y., Koonin E.V., Makarova K.S., Wolf Y.I., Rigden D.J., Dunfield P.F., Wang L., Alam M. Genome Biol. 9:R161.1-R161.16(2008) [PubMed: 19014707] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 21510 / WK1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000922 Genomic DNA. Translation: ACJ34171.1.
RefSeq	YP_002316156.1. NC_011567.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	B7GFB0.
Protein family/group databases
MEROPS	A08.001.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7038063.
GenomeReviews	Gene locus Aflv_1810 in contig CP000922_GR.
KEGG	afl:Aflv_1810.
PATRIC	20955505. VBIAnoFla45531_1861.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG724422.
ProtClustDB	PRK00376.
Family and domain databases
HAMAP	MF_00161. LspA. [Tree]
InterPro	IPR001872. Peptidase_A8. [Graphical view]
KO	K03101.
Pfam	PF01252. Peptidase_A8. 1 hit. [Graphical view]
PRINTS	PR00781. LIPOSIGPTASE.
TIGRFAMs	TIGR00077. LspA. 1 hit.
PROSITE	PS00855. SPASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LSPA_ANOFW

Accession

Primary (citable) accession number: B7GFB0

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	February 10, 2009
Last modified:	January 25, 2012
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program