B7GF12 (HEMH_ANOFW) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ferrochelatase
EC=4.99.1.1

Alternative name(s):
Heme synthase
Protoheme ferro-lyase

Gene names

Name:	hemH
Ordered Locus Names:	Aflv_2279

Organism

Anoxybacillus flavithermus (strain DSM 21510 / WK1) [Complete proteome] [HAMAP]

Taxonomic identifier

491915 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Anoxybacillus

Protein attributes

Sequence length	315 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the ferrous insertion into protoporphyrin IX By similarity. HAMAP MF_00323
Catalytic activity	Protoheme + 2 H+ = protoporphyrin + Fe2+. HAMAP MF_00323
Pathway	Porphyrin metabolism; protoheme biosynthesis; protoheme from protoporphyrin-IX: step 1/1. HAMAP MF_00323
Subcellular location	Cytoplasm By similarity HAMAP MF_00323.
Sequence similarities	Belongs to the ferrochelatase family.

Ontologies

Keywords
Biological process	Heme biosynthesis Porphyrin biosynthesis
Cellular component	Cytoplasm
Ligand	Iron Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	heme biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ferrochelatase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 315	315	Ferrochelatase HAMAP MF_00323		PRO_1000119601
Sites
	Metal binding	183	1	Iron By similarity
	Metal binding	264	1	Iron By similarity

Sequences

	Sequence		Length	Mass (Da)
	B7GF12 [UniParc]. Last modified February 10, 2009. Version 1. Checksum: 27C93149041520E0	FASTA	315	35,885
	10 20 30 40 50 60 MSKKVMGLLV MAYGTPYKEE DLERYYTHIR HGRKPSEEML ADLRERYEAI GGISPLAAIT 70 80 90 100 110 120 KQQAEKLAER LNEVQDEIEF RMYLGLKHIE PFVEDAVQQM HEDGIEEAVS IVLAPHFSTF 130 140 150 160 170 180 SVKSYNGRAK EEAARLGGPK LTCVESWYTE PKFIQYWADR VKETYASMSE REREKAVLIV 190 200 210 220 230 240 SAHSLPEKII AMGDPYPKQL QETADFIAKE AGVSEYVIGW QSAGNTPEPW LGPDVQDLTR 250 260 270 280 290 300 QLYEEKGYEA FVYVPAGFVS DHLEVLYDND IECKQVTDEL GVSYYRPPMP NAHPQFIDAL 310 ATVVLNHLRK EGESL

References

[1]

"Encapsulated in silica: genome, proteome and physiology of the thermophilic bacterium Anoxybacillus flavithermus WK1." Saw J.H., Mountain B.W., Feng L., Omelchenko M.V., Hou S., Saito J.A., Stott M.B., Li D., Zhao G., Wu J., Galperin M.Y., Koonin E.V., Makarova K.S., Wolf Y.I., Rigden D.J., Dunfield P.F., Wang L., Alam M. Genome Biol. 9:R161.1-R161.16(2008) [PubMed: 19014707] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 21510 / WK1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000922 Genomic DNA. Translation: ACJ34636.1.
RefSeq	YP_002316621.1. NC_011567.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	B7GF12.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7038531.
GenomeReviews	Gene locus Aflv_2279 in contig CP000922_GR.
KEGG	afl:Aflv_2279.
PATRIC	20956480. VBIAnoFla45531_2347.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG697135.
ProtClustDB	PRK12435.
Family and domain databases
HAMAP	MF_00323. Ferrochelatase. [Tree]
InterPro	IPR001015. Ferrochelatase. IPR019772. Ferrochelatase_AS. [Graphical view]
KO	K01772.
PANTHER	PTHR11108. Ferrochelatase. 1 hit.
Pfam	PF00762. Ferrochelatase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00109. HemH. 1 hit.
PROSITE	PS00534. FERROCHELATASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HEMH_ANOFW

Accession

Primary (citable) accession number: B7GF12

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	February 10, 2009
Last modified:	January 25, 2012
This is version 20 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program