ID   B7G725_PHATC            Unreviewed;       877 AA.
AC   B7G725;
DT   10-FEB-2009, integrated into UniProtKB/TrEMBL.
DT   10-FEB-2009, sequence version 1.
DT   24-JAN-2024, entry version 61.
DE   RecName: Full=phosphoenolpyruvate carboxylase {ECO:0000256|ARBA:ARBA00012305};
DE            EC=4.1.1.31 {ECO:0000256|ARBA:ARBA00012305};
GN   Name=PEPCase_2 {ECO:0000313|EMBL:EEC45565.1};
GN   ORFNames=PHATRDRAFT_51136 {ECO:0000313|EMBL:EEC45565.1};
OS   Phaeodactylum tricornutum (strain CCAP 1055/1).
OC   Eukaryota; Sar; Stramenopiles; Ochrophyta; Bacillariophyta;
OC   Bacillariophyceae; Bacillariophycidae; Naviculales; Phaeodactylaceae;
OC   Phaeodactylum.
OX   NCBI_TaxID=556484 {ECO:0000313|EMBL:EEC45565.1, ECO:0000313|Proteomes:UP000000759};
RN   [1] {ECO:0000313|EMBL:EEC45565.1, ECO:0000313|Proteomes:UP000000759}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CCAP 1055/1 {ECO:0000313|EMBL:EEC45565.1,
RC   ECO:0000313|Proteomes:UP000000759};
RX   PubMed=18923393; DOI=10.1038/nature07410;
RA   Bowler C., Allen A.E., Badger J.H., Grimwood J., Jabbari K., Kuo A.,
RA   Maheswari U., Martens C., Maumus F., Otillar R.P., Rayko E., Salamov A.,
RA   Vandepoele K., Beszteri B., Gruber A., Heijde M., Katinka M., Mock T.,
RA   Valentin K., Verret F., Berges J.A., Brownlee C., Cadoret J.P.,
RA   Chiovitti A., Choi C.J., Coesel S., De Martino A., Detter J.C., Durkin C.,
RA   Falciatore A., Fournet J., Haruta M., Huysman M.J., Jenkins B.D.,
RA   Jiroutova K., Jorgensen R.E., Joubert Y., Kaplan A., Kroger N., Kroth P.G.,
RA   La Roche J., Lindquist E., Lommer M., Martin-Jezequel V., Lopez P.J.,
RA   Lucas S., Mangogna M., McGinnis K., Medlin L.K., Montsant A.,
RA   Oudot-Le Secq M.P., Napoli C., Obornik M., Parker M.S., Petit J.L.,
RA   Porcel B.M., Poulsen N., Robison M., Rychlewski L., Rynearson T.A.,
RA   Schmutz J., Shapiro H., Siaut M., Stanley M., Sussman M.R., Taylor A.R.,
RA   Vardi A., von Dassow P., Vyverman W., Willis A., Wyrwicz L.S.,
RA   Rokhsar D.S., Weissenbach J., Armbrust E.V., Green B.R., Van de Peer Y.,
RA   Grigoriev I.V.;
RT   "The Phaeodactylum genome reveals the evolutionary history of diatom
RT   genomes.";
RL   Nature 456:239-244(2008).
RN   [2] {ECO:0000313|Proteomes:UP000000759}
RP   GENOME REANNOTATION.
RC   STRAIN=CCAP 1055/1 {ECO:0000313|Proteomes:UP000000759};
RG   Diatom Consortium;
RA   Grigoriev I., Grimwood J., Kuo A., Otillar R.P., Salamov A., Detter J.C.,
RA   Lindquist E., Shapiro H., Lucas S., Glavina del Rio T., Pitluck S.,
RA   Rokhsar D., Bowler C.;
RL   Submitted (AUG-2008) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=oxaloacetate + phosphate = hydrogencarbonate +
CC         phosphoenolpyruvate; Xref=Rhea:RHEA:28370, ChEBI:CHEBI:16452,
CC         ChEBI:CHEBI:17544, ChEBI:CHEBI:43474, ChEBI:CHEBI:58702; EC=4.1.1.31;
CC         Evidence={ECO:0000256|ARBA:ARBA00001071};
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000256|ARBA:ARBA00001946};
CC   -!- SIMILARITY: Belongs to the PEPCase type 1 family.
CC       {ECO:0000256|ARBA:ARBA00008346}.
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DR   EMBL; CM000619; EEC45565.1; -; Genomic_DNA.
DR   RefSeq; XP_002182829.1; XM_002182793.1.
DR   AlphaFoldDB; B7G725; -.
DR   SMR; B7G725; -.
DR   STRING; 556484.B7G725; -.
DR   PaxDb; 2850-Phatr51136; -.
DR   GeneID; 7203602; -.
DR   KEGG; pti:PHATRDRAFT_51136; -.
DR   eggNOG; ENOG502QPVS; Eukaryota.
DR   HOGENOM; CLU_006557_2_0_1; -.
DR   InParanoid; B7G725; -.
DR   OrthoDB; 355614at2759; -.
DR   Proteomes; UP000000759; Chromosome 17.
DR   GO; GO:0008964; F:phosphoenolpyruvate carboxylase activity; IEA:UniProtKB-EC.
DR   GO; GO:0015977; P:carbon fixation; IEA:UniProtKB-KW.
DR   GO; GO:0006099; P:tricarboxylic acid cycle; IEA:InterPro.
DR   Gene3D; 1.20.1440.90; Phosphoenolpyruvate/pyruvate domain; 1.
DR   HAMAP; MF_00595; PEPcase_type1; 1.
DR   InterPro; IPR021135; PEP_COase.
DR   InterPro; IPR022805; PEP_COase_bac/pln-type.
DR   InterPro; IPR018129; PEP_COase_Lys_AS.
DR   InterPro; IPR033129; PEPCASE_His_AS.
DR   InterPro; IPR015813; Pyrv/PenolPyrv_Kinase-like_dom.
DR   PANTHER; PTHR30523; PHOSPHOENOLPYRUVATE CARBOXYLASE; 1.
DR   PANTHER; PTHR30523:SF6; PHOSPHOENOLPYRUVATE CARBOXYLASE; 1.
DR   Pfam; PF00311; PEPcase; 1.
DR   PRINTS; PR00150; PEPCARBXLASE.
DR   SUPFAM; SSF51621; Phosphoenolpyruvate/pyruvate domain; 1.
DR   PROSITE; PS00781; PEPCASE_1; 1.
DR   PROSITE; PS00393; PEPCASE_2; 1.
PE   3: Inferred from homology;
KW   Carbon dioxide fixation {ECO:0000256|ARBA:ARBA00023300};
KW   Lyase {ECO:0000256|ARBA:ARBA00023239};
KW   Magnesium {ECO:0000256|ARBA:ARBA00022842};
KW   Reference proteome {ECO:0000313|Proteomes:UP000000759}.
FT   REGION          37..58
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        94
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU10111"
FT   ACT_SITE        531
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU10112"
SQ   SEQUENCE   877 AA;  98351 MW;  D37F3D64690B7E10 CRC64;
     MIDAASKLTA TEALGVTRVF SIMLNLVNAA EVQHRNRQIR AHESTKDPSG GPLPKTEDSI
     RGTMETLLES KQATPEEIFA QLQKQKVEIV LTAHPTQVQR KSLLRKYRRV SEMLAYLERP
     DLDGFEKSSA QTSLQTILSS IWGADEIRRQ KPTPQQEAAG GNAILESVLW DAVPAYLRKL
     DQQCRLTLGQ SLPVDVCPIK FASWIGGDRD GNPNVTPEVT REVVLQQRLR AARLLLKDMY
     DLISELAISS RFSPAMDALA DSVKDSQHKR EKYRRVIGHL IKRLVKTARE CELELSKLNT
     SASMVSQTLV EEAVDGWQDV DALDDATDLI KPLRIMYDSL VETGFGLVAD GLLVDIIRRL
     YVFGMSLVPL DIREESTKHT EALDAITRWL GIGSYSEWTE EARLSWLTSE LSNKRPLYRI
     RELPKLGFND SVLKTLNVFG TIATLRPSCL GAYVISQAQT ASDVLAVMLL QKQYGMTDKN
     RNMMRVVPLF ETLNDLTNAP DKLEQLFSIP LYVGAVKGKQ EVMVGYSDSA KDAGRLAACW
     AQYNSQERMV KVAAKHNIEL TFFHGKGGTV GRGGNPSVYR AIMSHPPNTI NGRFRVTEQG
     EMITQNFGAP SIAERTLDIY TAGVCREAFS ERVEPSQAWR DQMQRISDVS CAEYRHLVRE
     EPRFVPYFRQ ATPELELGSL NIGSRPAKRN PKGGIESLRA IPWTFAWTQT RTHLSAWLGV
     GAGLTTTDQS ELKTLRAMYI EWPWFRETID LIAMIVSKTD FSISKNYDDQ LVEKKEGLLK
     LGDEVREKMV QTRQAVLDVT ESTDVAGAHV ALMRGSSTIR HPYVDPVNVI QAELLKRLRV
     MDKKKSLLAD EMEEQEILKD ALIISINGIA QGMRNSG
//