B6ZZZ3 (B6ZZZ3_ECO57) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Dihydroorotase HAMAP MF_00219
Short name=DHOase HAMAP MF_00219
EC=3.5.2.3 HAMAP MF_00219

Gene names

Name:	pyrC HAMAP MF_00219 EMBL EEC27108.1
ORF Names:	ESCCO14588_2893 EMBL EEC27108.1

Organism

Escherichia coli O157:H7 str. TW14588 EMBL EEC27108.1

Taxonomic identifier

502346 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	348 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	(S)-dihydroorotate + H2O = N-carbamoyl-L-aspartate. HAMAP MF_00219 RuleBase RU003440 SAAS SAAS004721
Cofactor	Binds 2 zinc ions per subunit By similarity. HAMAP MF_00219 RuleBase RU003440 SAAS SAAS004721
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 3/3. HAMAP MF_00219 RuleBase RU003440 SAAS SAAS004721
Subunit structure	Homodimer By similarity. HAMAP MF_00219 SAAS SAAS004721
Sequence similarities	Belongs to the DHOase family. Type 1 subfamily. HAMAP MF_00219 RuleBase RU003440

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis HAMAP MF_00219 RuleBase RU003440 SAAS SAAS004721
Ligand	Metal-binding SAAS SAAS004721 HAMAP MF_00219 RuleBase RU003440 Zinc HAMAP MF_00219 RuleBase RU003440 SAAS SAAS004721
Molecular function	Hydrolase HAMAP MF_00219 RuleBase RU003440 SAAS SAAS004721 EMBL EEC27108.1
Gene Ontology (GO)
Biological process	pyrimidine base biosynthetic process Inferred from electronic annotation. Source: InterPro pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	dihydroorotase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Metal binding	17	1	Zinc 1 By similarity HAMAP MF_00219
	Metal binding	19	1	Zinc 1 By similarity HAMAP MF_00219
	Metal binding	103	1	Zinc 1; via carbamate group By similarity HAMAP MF_00219
	Metal binding	103	1	Zinc 2; via carbamate group By similarity HAMAP MF_00219
	Metal binding	140	1	Zinc 2 By similarity HAMAP MF_00219
	Metal binding	178	1	Zinc 2 By similarity HAMAP MF_00219
	Metal binding	251	1	Zinc 1 By similarity HAMAP MF_00219
Amino acid modifications
	Modified residue	103	1	N6-carboxylysine By similarity HAMAP MF_00219

Sequences

	Sequence		Length	Mass (Da)
	B6ZZZ3 [UniParc]. Last modified February 10, 2009. Version 1. Checksum: 057D1F6834446CA7	FASTA	348	38,844
	10 20 30 40 50 60 MTAPSQVLKI RRPDDWHLHL RDGDMLKTVV PYTSEIYGRA IVMPNLAPPV TTVEAAVAYR 70 80 90 100 110 120 QRILHAVPAG HDFTPLMTCY LTDSLDPNEL ERGFNEGVFT AAKLYPANAT TNSSHGVTSV 130 140 150 160 170 180 DAIMPVLERM EKIGMPLLVH GEVTHADIDI FDREARFIES VMEPLRQRLT ALKVVFEHIT 190 200 210 220 230 240 TKDAADYVRD GNERLAATIT PQHLMFNRNH MLVGGVRPHL YCLPILKRNI HQQALRELVA 250 260 270 280 290 300 SGFNRVFLGT DSAPHARHRK ESSCGCAGCF NAPTALGSYA TVFEEMNALQ HFEAFCSVNG 310 320 330 340 PQFYGLPVND TFIELVREEH QVAESIALTD DTLVPFLAGE TVRWSVKQ

References

[1]	Sebastian Y., Whittam T.S., Manning S.D., Sutton G. Submitted (NOV-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: TW14588 EMBL EEC27108.1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	ABKY02000003 Genomic DNA. Translation: EEC27108.1.
3D structure databases
ProteinModelPortal	B6ZZZ3.
SMR	B6ZZZ3. Positions 4-347.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000223744; EBESCP00000211260; EBESCG00000225289.
PATRIC	29408193. VBIEscCol82497_2845.
Phylogenomic databases
OMA	CLPVAKR.
Family and domain databases
HAMAP	MF_00219. PyrC_type1. [Tree]
InterPro	IPR006680. Amidohydro_1. IPR004721. DHOdimr. IPR002195. Dihydroorotase_CS. [Graphical view]
Pfam	PF01979. Amidohydro_1. 1 hit. [Graphical view]
PIRSF	PIRSF001237. DHOdimr. 1 hit.
TIGRFAMs	TIGR00856. PyrC_dimer. 1 hit.
PROSITE	PS00482. DIHYDROOROTASE_1. 1 hit. PS00483. DIHYDROOROTASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

B6ZZZ3_ECO57

Accession

Primary (citable) accession number: B6ZZZ3

Entry history

Integrated into UniProtKB/TrEMBL:	February 10, 2009
Last sequence update:	February 10, 2009
Last modified:	December 14, 2011
This is version 20 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)