B6ZT36 (B6ZT36_ECO57) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
RNA 3'-terminal phosphate cyclase HAMAP MF_00200
Short name=RNA cyclase HAMAP MF_00200
Short name=RNA-3'-phosphate cyclase HAMAP MF_00200
EC=6.5.1.4 HAMAP MF_00200

Gene names

Name:	rtcA HAMAP MF_00200 EMBL EEC29708.1
ORF Names:	ESCCO14588_0127 EMBL EEC29708.1

Organism

Escherichia coli O157:H7 str. TW14588 EMBL EEC29708.1

Taxonomic identifier

502346 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	342 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) transfer of adenylate to an RNA-N3'P to produce RNA-N3'PP5'A; (C) and attack of the adjacent 2'-hydroxyl on the 3'-phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing By similarity. HAMAP MF_00200
Catalytic activity	ATP + RNA 3'-terminal-phosphate = AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate. HAMAP MF_00200
Subcellular location	Cytoplasm By similarity HAMAP MF_00200.
Sequence similarities	Belongs to the RNA 3'-terminal cyclase family. Type 1 subfamily. HAMAP MF_00200

Ontologies

Keywords
Cellular component	Cytoplasm HAMAP MF_00200
Ligand	ATP-binding HAMAP MF_00200 Nucleotide-binding
Molecular function	Ligase HAMAP MF_00200 EMBL EEC29708.1
Gene Ontology (GO)
Biological process	RNA processing Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA-3'-phosphate cyclase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Nucleotide binding	283 – 287	5	ATP By similarity HAMAP MF_00200
Sites
	Active site	308	1	Tele-AMP-histidine intermediate By similarity HAMAP MF_00200
	Binding site	103	1	ATP By similarity HAMAP MF_00200

Sequences

	Sequence		Length	Mass (Da)
	B6ZT36 [UniParc]. Last modified February 10, 2009. Version 1. Checksum: 783FE7FAD7160846	FASTA	342	36,332
	10 20 30 40 50 60 MKRMIALDGA QGEGGGQIMR SALSLSMITG QPFTITGIRA GRAKPGLLRQ HLTAVKAATE 70 80 90 100 110 120 ICGATVEGAE LGSQRLVFRP GTVRGGDYRF AIGSAGSCTL VLQTVLPALW FADGPSRVEV 130 140 150 160 170 180 SGGTDNPSAP PADFIRRVLE PLLAKIGIHQ QTTLLRHGFY PAGGGVVATE VSPVASFNTL 190 200 210 220 230 240 QLGERGNIVQ MRGEVLLAGV PRHVAEREIA TLAASFSLHE QNIHNLPRDQ GPGNTVSLEV 250 260 270 280 290 300 ESENITERFF VVGEKRVSAE VVAAQLVKEV KRYLASPAAV GEYLADQLVL PMALAGAGQF 310 320 330 340 TVAHPSCHLL TNIAVVERFL PVRFTLAETD GVTRVMITKL TD

References

[1]	Sebastian Y., Whittam T.S., Manning S.D., Sutton G. Submitted (NOV-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: TW14588 EMBL EEC29708.1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	ABKY02000001 Genomic DNA. Translation: EEC29708.1.
3D structure databases
ProteinModelPortal	B6ZT36.
SMR	B6ZT36. Positions 4-337.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000226453; EBESCP00000211093; EBESCG00000225956.
PATRIC	29402065. VBIEscCol82497_2389.
Phylogenomic databases
OMA	GGTDVAW.
Family and domain databases
HAMAP	MF_00200. RTC. [Tree]
InterPro	IPR013791. RNA3'-term_phos_cycl_insert. IPR023797. RNA3'_phos_cyclase_dom. IPR000228. RNA3'_term_phos_cyc. IPR017770. RNA3'_term_phos_cyc_type_1. IPR020719. RNA3'_term_phos_cycl-like_CS. IPR013796. RNA3'_term_phos_cycl_insert. IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b. [Graphical view]
Gene3D	G3DSA:3.30.360.20. G3DSA:3.30.360.20. 1 hit. G3DSA:3.65.10.20. RNA3'_term_phos_cycl. 2 hits.
PANTHER	PTHR11096. RNA3'_term_phos_cycl. 1 hit.
Pfam	PF01137. RTC. 1 hit. PF05189. RTC_insert. 1 hit. [Graphical view]
SUPFAM	SSF52913. RNA3'-term_phos_cycl_insert. 1 hit. SSF55205. RNA3'_cycl/enolpyr_transf_A/B. 2 hits.
TIGRFAMs	TIGR03399. RNA_3prim_cycl. 1 hit.
PROSITE	PS01287. RTC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

B6ZT36_ECO57

Accession

Primary (citable) accession number: B6ZT36

Entry history

Integrated into UniProtKB/TrEMBL:	February 10, 2009
Last sequence update:	February 10, 2009
Last modified:	December 14, 2011
This is version 16 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)