B6YXZ4 (B6YXZ4_THEON) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Methylated-DNA--protein-cysteine methyltransferase HAMAP-Rule MF_00772
EC=2.1.1.63 HAMAP-Rule MF_00772

Alternative name(s):
6-O-methylguanine-DNA methyltransferase HAMAP-Rule MF_00772
O-6-methylguanine-DNA-alkyltransferase HAMAP-Rule MF_00772

Gene names

Name:	ogt HAMAP-Rule MF_00772
Ordered Locus Names:	TON_1467

Organism

Thermococcus onnurineus (strain NA1) [Complete proteome] [HAMAP] EMBL ACJ16957.1

Taxonomic identifier

523850 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Thermococcus › Thermococcus onnurineus

Protein attributes

Sequence length	179 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated By similarity. HAMAP-Rule MF_00772
Catalytic activity	DNA (containing 6-O-methylguanine) + protein L-cysteine = DNA (without 6-O-methylguanine) + protein S-methyl-L-cysteine. HAMAP-Rule MF_00772
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00772.
Miscellaneous	This enzyme catalyzes only one turnover and therefore is not strictly catalytic. According to one definition, an enzyme is a biocatalyst that acts repeatedly and over many reaction cycles By similarity. HAMAP-Rule MF_00772
Sequence similarities	Belongs to the MGMT family. HAMAP-Rule MF_00772

Ontologies

Keywords
Biological process	DNA damage DNA repair HAMAP-Rule MF_00772
Cellular component	Cytoplasm HAMAP-Rule MF_00772
Molecular function	Methyltransferase HAMAP-Rule MF_00772 EMBL ACJ16957.1 Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	DNA dealkylation involved in DNA repair Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	methylated-DNA-[protein]-cysteine S-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Active site	142	1	Nucleophile; methyl group acceptor By similarity HAMAP-Rule MF_00772

Sequences

	Sequence		Length	Mass (Da)
	B6YXZ4 [UniParc]. Last modified January 20, 2009. Version 1. Checksum: 917AACF8FEA86ECE	FASTA	179	20,532
	10 20 30 40 50 60 MLSVERFDIA RKEVWIAVIF DEKIQGITFS LDGEGFLRER IANMAFFLKK RGVNVELTLL 70 80 90 100 110 120 ESDYPRLVRD VILGKLENEE LLPELGFLGV TSFERRVYEW LTKRVKRGSV VTYGELAKTL 130 140 150 160 170 GTSPRAIGGA MKRNPYPIVV PCHRVVAWDG PGYYTPKLEY KAFLLKLEGV EEWTSSKRT

References

[1]

"The complete genome sequence of Thermococcus onnurineus NA1 reveals a mixed heterotrophic and carboxydotrophic metabolism." Lee H.S., Kang S.G., Bae S.S., Lim J.K., Cho Y., Kim Y.J., Jeon J.H., Cha S.-S., Kwon K.K., Kim H.-T., Park C.-J., Lee H.-W., Kim S.I., Chun J., Colwell R.R., Kim S.-J., Lee J.-H. J. Bacteriol. 190:7491-7499(2008) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NA1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000855 Genomic DNA. Translation: ACJ16957.1.
RefSeq	YP_002307854.1. NC_011529.1.
3D structure databases
ProteinModelPortal	B6YXZ4.
ModBase	Search...
Protein-protein interaction databases
STRING	523850.TON_1467.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACJ16957; ACJ16957; TON_1467.
GeneID	7018502.
KEGG	ton:TON_1467.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0350.
HOGENOM	HOG000244137.
KO	K00567.
OMA	KRNPYPI.
ProtClustDB	PRK03887.
Family and domain databases
Gene3D	1.10.10.10. 1 hit.
HAMAP	MF_00772. OGT.
InterPro	IPR015236. DUF1938. IPR001497. MethylDNA_cys_MeTrfase_AS. IPR014048. MethylDNA_cys_MeTrfase_DNA-bd. IPR023546. MGMT. IPR011991. WHTH_DNA-bd_dom. [Graphical view]
Pfam	PF01035. DNA_binding_1. 1 hit. PF09153. DUF1938. 1 hit. [Graphical view]
ProDom	PD068053. DUF1938. 1 hit. [Graphical view] [Entries sharing at least one domain]
SUPFAM	SSF46767. MethylDNA_cys_mtrans_DNA_bd. 1 hit.
TIGRFAMs	TIGR00589. ogt. 1 hit.
PROSITE	PS00374. MGMT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

B6YXZ4_THEON

Accession

Primary (citable) accession number: B6YXZ4

Entry history

Integrated into UniProtKB/TrEMBL:	January 20, 2009
Last sequence update:	January 20, 2009
Last modified:	May 1, 2013
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)