B6YXU4 (ENDA_THEON) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA-splicing endonuclease
EC=3.1.27.9

Alternative name(s):
tRNA-intron endonuclease

Gene names

Name:	endA
Ordered Locus Names:	TON_1417

Organism

Thermococcus onnurineus (strain NA1) [Complete proteome] [HAMAP]

Taxonomic identifier

523850 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Thermococcus › Thermococcus onnurineus

Protein attributes

Sequence length	171 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp By similarity. HAMAP-Rule MF_01833
Catalytic activity	Endonucleolytic cleavage of pre-tRNA, producing 5'-hydroxy and 2',3'-cyclic phosphate termini, and specifically removing the intron. HAMAP-Rule MF_01833
Subunit structure	Homotetramer; although the tetramer contains four active sites, only two participate in the cleavage. Therefore, it should be considered as a dimer of dimers By similarity.
Sequence similarities	Belongs to the tRNA-intron endonuclease family. Archaeal short subfamily.

Ontologies

Keywords
Biological process	tRNA processing
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC tRNA splicing, via endonucleolytic cleavage and ligation Inferred from electronic annotation. Source: HAMAP
Molecular_function	nucleic acid binding Inferred from electronic annotation. Source: InterPro tRNA-intron endonuclease activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 171	171	tRNA-splicing endonuclease HAMAP-Rule MF_01833		PRO_1000188343
Sites
	Active site	110	1	By similarity
	Active site	117	1	By similarity
	Active site	148	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	B6YXU4 [UniParc]. Last modified January 20, 2009. Version 1. Checksum: BEFA08021C7A99D9	FASTA	171	20,286
	10 20 30 40 50 60 MKEPIEFKLS GDRAFSEREK AINQLYNRRY FGEVVNGKLF LSLIEAAYLM ERGKIKVLDG 70 80 90 100 110 120 GKELSFEELF ELGRKKDDQF DIKYLVYKDL RDRGYIVKSA LKFGSHFRVY RRGMDEHSQW 130 140 150 160 170 LIWVVPENLR FSANDITARV RVAHGVRKNM VMAVVDEDND VVYYKIEWVK F

References

[1]

"The complete genome sequence of Thermococcus onnurineus NA1 reveals a mixed heterotrophic and carboxydotrophic metabolism." Lee H.S., Kang S.G., Bae S.S., Lim J.K., Cho Y., Kim Y.J., Jeon J.H., Cha S.-S., Kwon K.K., Kim H.-T., Park C.-J., Lee H.-W., Kim S.I., Chun J., Colwell R.R., Kim S.-J., Lee J.-H. J. Bacteriol. 190:7491-7499(2008) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NA1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000855 Genomic DNA. Translation: ACJ16907.1.
RefSeq	YP_002307804.1. NC_011529.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	523850.TON_1417.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACJ16907; ACJ16907; TON_1417.
GeneID	7018451.
KEGG	ton:TON_1417.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1676.
HOGENOM	HOG000107823.
KO	K01170.
OMA	FVRVAHS.
ProtClustDB	PRK09297.
Family and domain databases
Gene3D	3.40.1350.10. 1 hit.
HAMAP	MF_01833. EndA_short.
InterPro	IPR011856. tRNA_endonuc-like_dom. IPR006677. tRNA_intron_Endonuc_cat-like. IPR006678. tRNA_intron_Endonuc_N. IPR006676. tRNA_splic. IPR016442. tRNA_splic_arch_short. [Graphical view]
Pfam	PF01974. tRNA_int_endo. 1 hit. PF02778. tRNA_int_endo_N. 1 hit. [Graphical view]
PIRSF	PIRSF005285. tRNA_splic_archaea. 1 hit.
SUPFAM	SSF53032. tRNA_int_endo_C. 1 hit. SSF55267. tRNA_int_endo_N. 1 hit.
TIGRFAMs	TIGR00324. endA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ENDA_THEON

Accession

Primary (citable) accession number: B6YXU4

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	January 20, 2009
Last modified:	May 1, 2013
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program