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B6YXA9 (RBL_THEON) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 38. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ribulose bisphosphate carboxylase

Short name=RuBisCO
EC=4.1.1.39
Gene names
Name:rbcL
Ordered Locus Names:TON_1234
OrganismThermococcus onnurineus (strain NA1) [Complete proteome] [HAMAP]
Taxonomic identifier523850 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaeThermococcus

Protein attributes

Sequence length444 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the addition of molecular CO2 and H2O to ribulose 1,5-bisphosphate (RuBP), generating two molecules of 3-phosphoglycerate (3-PGA). Functions in an archaeal AMP degradation pathway, together with AMP phosphorylase and R15P isomerase By similarity. HAMAP-Rule MF_01133

Catalytic activity

2 3-phospho-D-glycerate + 2 H+ = D-ribulose 1,5-bisphosphate + CO2 + H2O. HAMAP-Rule MF_01133

3-phospho-D-glycerate + 2-phosphoglycolate = D-ribulose 1,5-bisphosphate + O2. HAMAP-Rule MF_01133

Cofactor

Binds 1 magnesium ion per subunit By similarity. HAMAP-Rule MF_01133

Subunit structure

Homodimer or homodecamer. In contrast to form I RuBisCO, the form III RuBisCO is composed solely of large subunits By similarity.

Miscellaneous

Because the Archaea possessing a type III RuBisCO are all anaerobic, it is most likely that only the carboxylase activity of RuBisCO, and not the competitive oxygenase activity (by which RuBP reacts with O2 to form one molecule of 3-phosphoglycerate and one molecule of 2-phosphoglycolate), is biologically relevant in these strains (PubMed:17303759).

Sequence similarities

Belongs to the RuBisCO large chain family. Type III subfamily.

Ontologies

Keywords
   Biological processCarbon dioxide fixation
   LigandMagnesium
Metal-binding
   Molecular functionLyase
Oxidoreductase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processAMP catabolic process

Inferred from electronic annotation. Source: UniProtKB-HAMAP

carbon fixation

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionmagnesium ion binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

oxidoreductase activity

Inferred from electronic annotation. Source: UniProtKB-KW

ribulose-bisphosphate carboxylase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 444444Ribulose bisphosphate carboxylase HAMAP-Rule MF_01133
PRO_1000137340

Regions

Region367 – 3693Substrate binding By similarity
Region389 – 3924Substrate binding By similarity

Sites

Active site1631Proton acceptor By similarity
Active site2811Proton acceptor By similarity
Metal binding1891Magnesium; via carbamate group By similarity
Metal binding1911Magnesium By similarity
Metal binding1921Magnesium By similarity
Binding site1651Substrate By similarity
Binding site2821Substrate By similarity
Binding site3141Substrate By similarity
Site3221Transition state stabilizer By similarity

Amino acid modifications

Modified residue1891N6-carboxylysine By similarity

Sequences

Sequence LengthMass (Da)Tools
B6YXA9 [UniParc].

Last modified January 20, 2009. Version 1.
Checksum: 69F7078ECAF9346D

FASTA44449,875
        10         20         30         40         50         60 
MVEKFDKIYD YYVDKSYEPN KKRDIIAVFR ITPAEGYTIE QVAGGVAAES STGTWTTLYN 

        70         80         90        100        110        120 
WYEEERWADL SAKAYDFIDM GDGSWIVKIA YPFHAFEEAN LPGLLASIAG NVFGMRRAKG 

       130        140        150        160        170        180 
LRLEDMYFPE KLIREFSGPA FGIEGVRKML EIKDRPIYGV VPKPKVGYSP EEFEKLSYEL 

       190        200        210        220        230        240 
LLNGADYMKD DENLTSPWYN RFEERAETIA KIIEKVESET GEKKTWFANI TADVREMERR 

       250        260        270        280        290        300 
LEILADLGLK HAMVDVVITG WGALEYIRDL AADYGLAIHG HRAMHATFTR NPYHGISMFV 

       310        320        330        340        350        360 
LAKLYRLIGI DQLHVGTAGA GKLEGGKWDV IQNARILREE HYKPDENDVF HLEQKFYSIK 

       370        380        390        400        410        420 
PAFPTSSGGL HPGNLPIVID ALGTDIVLQL GGGTLGHPDG PAAGARAVRQ AIDALVQGIP 

       430        440 
LDEYAKTHKE LARALEKWGH VTPI 

« Hide

References

[1]"The complete genome sequence of Thermococcus onnurineus NA1 reveals a mixed heterotrophic and carboxydotrophic metabolism."
Lee H.S., Kang S.G., Bae S.S., Lim J.K., Cho Y., Kim Y.J., Jeon J.H., Cha S.-S., Kwon K.K., Kim H.-T., Park C.-J., Lee H.-W., Kim S.I., Chun J., Colwell R.R., Kim S.-J., Lee J.-H.
J. Bacteriol. 190:7491-7499(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: NA1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000855 Genomic DNA. Translation: ACJ16722.1.
RefSeqYP_002307619.1. NC_011529.1.

3D structure databases

ProteinModelPortalB6YXA9.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING523850.TON_1234.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACJ16722; ACJ16722; TON_1234.
GeneID7018257.
KEGGton:TON_1234.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1850.
HOGENOMHOG000230831.
KOK01601.
OMAHRAMHAA.
ProtClustDBPRK04208.

Enzyme and pathway databases

BioCycTONN523850:GC7X-1267-MONOMER.

Family and domain databases

Gene3D3.20.20.110. 1 hit.
3.30.70.150. 1 hit.
HAMAPMF_01133. RuBisCO_L_type3.
InterProIPR017712. RuBisCO_III.
IPR000685. RuBisCO_lsu_C.
IPR017443. RuBisCO_lsu_fd_N.
IPR017444. RuBisCO_lsu_N.
[Graphical view]
PfamPF00016. RuBisCO_large. 1 hit.
PF02788. RuBisCO_large_N. 1 hit.
[Graphical view]
SUPFAMSSF51649. SSF51649. 1 hit.
SSF54966. SSF54966. 1 hit.
TIGRFAMsTIGR03326. rubisco_III. 1 hit.
ProtoNetSearch...

Entry information

Entry nameRBL_THEON
AccessionPrimary (citable) accession number: B6YXA9
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: January 20, 2009
Last modified: February 19, 2014
This is version 38 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families