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Protein

Ribulose bisphosphate carboxylase

Gene

rbcL

Organism
Thermococcus onnurineus (strain NA1)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the addition of molecular CO2 and H2O to ribulose 1,5-bisphosphate (RuBP), generating two molecules of 3-phosphoglycerate (3-PGA). Functions in an archaeal AMP degradation pathway, together with AMP phosphorylase and R15P isomerase.UniRule annotation

Miscellaneous

Because the Archaea possessing a type III RuBisCO are all anaerobic, it is most likely that only the carboxylase activity of RuBisCO, and not the competitive oxygenase activity (by which RuBP reacts with O2 to form one molecule of 3-phosphoglycerate and one molecule of 2-phosphoglycolate), is biologically relevant in these strains.UniRule annotation

Catalytic activityi

2 3-phospho-D-glycerate + 2 H+ = D-ribulose 1,5-bisphosphate + CO2 + H2O.UniRule annotation
3-phospho-D-glycerate + 2-phosphoglycolate = D-ribulose 1,5-bisphosphate + O2.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei163Proton acceptorUniRule annotation1
Binding sitei165SubstrateUniRule annotation1
Metal bindingi189Magnesium; via carbamate groupUniRule annotation1
Metal bindingi191MagnesiumUniRule annotation1
Metal bindingi192MagnesiumUniRule annotation1
Active sitei281Proton acceptorUniRule annotation1
Binding sitei282SubstrateUniRule annotation1
Binding sitei314SubstrateUniRule annotation1
Sitei322Transition state stabilizerUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase, Oxidoreductase
Biological processCarbon dioxide fixation
LigandMagnesium, Metal-binding

Enzyme and pathway databases

BioCyciTONN523850:G1GAB-1266-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Ribulose bisphosphate carboxylaseUniRule annotation (EC:4.1.1.39UniRule annotation)
Short name:
RuBisCOUniRule annotation
Gene namesi
Name:rbcLUniRule annotation
Ordered Locus Names:TON_1234
OrganismiThermococcus onnurineus (strain NA1)
Taxonomic identifieri523850 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaeThermococcus
Proteomesi
  • UP000002727 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001373401 – 444Ribulose bisphosphate carboxylaseAdd BLAST444

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei189N6-carboxylysineUniRule annotation1

Interactioni

Subunit structurei

Homodimer or homodecamer. In contrast to form I RuBisCO, the form III RuBisCO is composed solely of large subunits.UniRule annotation

Protein-protein interaction databases

STRINGi523850.TON_1234

Structurei

3D structure databases

ProteinModelPortaliB6YXA9
SMRiB6YXA9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni367 – 369Substrate bindingUniRule annotation3
Regioni389 – 392Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the RuBisCO large chain family. Type III subfamily.UniRule annotation

Phylogenomic databases

eggNOGiarCOG04443 Archaea
COG1850 LUCA
HOGENOMiHOG000230831
KOiK01601
OMAiHRAMHAA
OrthoDBiPOG093Z02MJ

Family and domain databases

CDDicd08213 RuBisCO_large_III, 1 hit
Gene3Di3.20.20.1101 hit
3.30.70.1501 hit
HAMAPiMF_01133 RuBisCO_L_type3, 1 hit
InterProiView protein in InterPro
IPR033966 RuBisCO
IPR017712 RuBisCO_III
IPR000685 RuBisCO_lsu_C
IPR036376 RuBisCO_lsu_C_sf
IPR017443 RuBisCO_lsu_fd_N
IPR036422 RuBisCO_lsu_N_sf
PfamiView protein in Pfam
PF00016 RuBisCO_large, 1 hit
PF02788 RuBisCO_large_N, 1 hit
SFLDiSFLDS00014 RuBisCO, 1 hit
SUPFAMiSSF51649 SSF51649, 1 hit
SSF54966 SSF54966, 1 hit
TIGRFAMsiTIGR03326 rubisco_III, 1 hit

Sequencei

Sequence statusi: Complete.

B6YXA9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVEKFDKIYD YYVDKSYEPN KKRDIIAVFR ITPAEGYTIE QVAGGVAAES
60 70 80 90 100
STGTWTTLYN WYEEERWADL SAKAYDFIDM GDGSWIVKIA YPFHAFEEAN
110 120 130 140 150
LPGLLASIAG NVFGMRRAKG LRLEDMYFPE KLIREFSGPA FGIEGVRKML
160 170 180 190 200
EIKDRPIYGV VPKPKVGYSP EEFEKLSYEL LLNGADYMKD DENLTSPWYN
210 220 230 240 250
RFEERAETIA KIIEKVESET GEKKTWFANI TADVREMERR LEILADLGLK
260 270 280 290 300
HAMVDVVITG WGALEYIRDL AADYGLAIHG HRAMHATFTR NPYHGISMFV
310 320 330 340 350
LAKLYRLIGI DQLHVGTAGA GKLEGGKWDV IQNARILREE HYKPDENDVF
360 370 380 390 400
HLEQKFYSIK PAFPTSSGGL HPGNLPIVID ALGTDIVLQL GGGTLGHPDG
410 420 430 440
PAAGARAVRQ AIDALVQGIP LDEYAKTHKE LARALEKWGH VTPI
Length:444
Mass (Da):49,875
Last modified:January 20, 2009 - v1
Checksum:i69F7078ECAF9346D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000855 Genomic DNA Translation: ACJ16722.1
RefSeqiWP_012572194.1, NC_011529.1

Genome annotation databases

EnsemblBacteriaiACJ16722; ACJ16722; TON_1234
GeneIDi7018257
KEGGiton:TON_1234
PATRICifig|523850.10.peg.1241

Similar proteinsi

Entry informationi

Entry nameiRBL_THEON
AccessioniPrimary (citable) accession number: B6YXA9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: January 20, 2009
Last modified: February 28, 2018
This is version 64 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome