Reviewed, UniProtKB/Swiss-Prot B6YWH0 (GYAR_THEON)

Names and origin

Protein names

Recommended name:
    Glyoxylate reductase
    EC=1.1.1.26

Gene names

Name:	gyaR
Ordered Locus Names:	TON_0945

Organism

Thermococcus onnurineus (strain NA1) [Complete proteome] [HAMAP]

Taxonomic identifier

523850 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Thermococcus

Protein attributes

Sequence length	334 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Glycolate + NAD+ = glyoxylate + NADH. HAMAP MF_00776
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. GyaR subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro glyoxylate reductase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 334	334	Glyoxylate reductase HAMAP MF_00776		PRO_1000201582
Regions
	Nucleotide binding	158 – 161	4	NADP By similarity
	Nucleotide binding	180 – 182	3	NADP By similarity
	Nucleotide binding	239 – 241	3	NADP By similarity
	Nucleotide binding	288 – 290	3	NADP By similarity
Sites
	Active site	241	1	By similarity
	Active site	270	1	By similarity
	Active site	288	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	B6YWH0-1 [UniParc]. Last modified January 20, 2009. Version 1. Checksum: 19FB2B93FD933EBF	FASTA	334	37,780
	10 20 30 40 50 60 MKPKVLITRK IPENGIKMLR EHFEVEVWED EHEISREVLL EKVRDVDALV TMLSERIDAE 70 80 90 100 110 120 VFDAAPRLKI VANYAVGYDN IDIEEATKMG VYITNTPDVL TNATADMAWV LLLATARRLI 130 140 150 160 170 180 EADKFVRSGE WKKRGVAWHP LMFLGYDVYG RTIGIVGFGR IGQAIARRAK GFGMRILYNS 190 200 210 220 230 240 RTRKPEVEKE LGAEFMPLDE LLKESDFVVL VVPLTKETYH MINEERLKLM KPTAILVNIA 250 260 270 280 290 300 RGKVVDTEAL VKALREGWIA GAGLDVFEEE PYYHEELFSL DNVVLAPHIG SATYGAREGM 310 320 330 AELVARNLIA FKNGEVPPTL VNREVLNVRK PGFE

References

[1]

"The complete genome sequence of Thermococcus onnurineus NA1 reveals a mixed heterotrophic and carboxydotrophic metabolism." Lee H.S., Kang S.G., Bae S.S., Lim J.K., Cho Y., Kim Y.J., Jeon J.H., Cha S.-S., Kwon K.K., Kim H.-T., Park C.-J., Lee H.-W., Kim S.I., Chun J., Colwell R.R., Kim S.-J., Lee J.-H. J. Bacteriol. 190:7491-7499(2008) [PubMed: 18790866] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000855 Genomic DNA. Translation: ACJ16433.1.
RefSeq	YP_002307330.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	7017248.
GenomeReviews	Gene locus TON_0945 in contig CP000855_GR.
KEGG	ton:TON_0945.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	ETRGMFG.
Family and domain databases
HAMAP	MF_00776. [Tree]
InterPro	IPR006139. D-isomer_2_OHA_DH. IPR006140. D-isomer_2_OHA_DH_NAD-bd. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF00389. 2-Hacid_dh. 1 hit. PF02826. 2-Hacid_dh_C. 1 hit. [Graphical view]
PROSITE	PS00065. D_2_HYDROXYACID_DH_1. False negative. PS00670. D_2_HYDROXYACID_DH_2. False negative. PS00671. D_2_HYDROXYACID_DH_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GYAR_THEON

Accession

Primary (citable) accession number: B6YWH0

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	January 20, 2009
Last modified:	November 3, 2009
This is version 12 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)