B6YV32 (B6YV32_THEON) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
NH(3)-dependent NAD(+) synthetase HAMAP MF_00193 RuleBase RU003812
EC=6.3.1.5 HAMAP MF_00193 RuleBase RU003812

Gene names

Name:	nadE HAMAP MF_00193
Ordered Locus Names:	TON_1770

Organism

Thermococcus onnurineus (strain NA1) [Complete proteome] [HAMAP]

Taxonomic identifier

523850 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Thermococcus

Protein attributes

Sequence length	253 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+. HAMAP MF_00193 RuleBase RU003812
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (ammonia route): step 1/1. HAMAP MF_00193 RuleBase RU004252
Sequence similarities	Belongs to the NAD synthetase family. HAMAP MF_00193 RuleBase RU003811

Ontologies

Keywords
Ligand	ATP-binding HAMAP MF_00193 RuleBase RU003811 NAD HAMAP MF_00193 RuleBase RU003811 Nucleotide-binding
Molecular function	Ligase HAMAP MF_00193 RuleBase RU003811
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro NAD biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP NAD+ synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro NAD+ synthase activity Inferred from electronic annotation. Source: HAMAP double-stranded DNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Nucleotide binding	32 – 39	8	ATP By similarity HAMAP MF_00193
Sites
	Active site	34	1	By similarity HAMAP MF_00193

Sequences

	Sequence		Length	Mass (Da)
	B6YV32 [UniParc]. Last modified January 20, 2009. Version 1. Checksum: 198200314600D5B7	FASTA	253	28,433
	10 20 30 40 50 60 MRSLDYDAVI ERIVSFIQGK VEEARANGVV VGVSGGIDSA TTAYLAVKAL GREKVLGLIM 70 80 90 100 110 120 PYYENGDVED AKLVCENLGI EYKLINIRPI VDEFEKAVGE LDVKSKGNIM ARTRMILLYA 130 140 150 160 170 180 HANSRNYLVL GTSNRSELLT GYFTKWGDGA SDYAPLINIY KTEVWELAKS LGVPERIIQK 190 200 210 220 230 240 KPTAGLWEGQ TDEDELGISY RLLDEILWRL IDLKMPKGEI AEELGIPLEK VEYVELLVKR 250 SEHKRRLPLG PEF

References

[1]

"The complete genome sequence of Thermococcus onnurineus NA1 reveals a mixed heterotrophic and carboxydotrophic metabolism." Lee H.S., Kang S.G., Bae S.S., Lim J.K., Cho Y., Kim Y.J., Jeon J.H., Cha S.-S., Kwon K.K., Kim H.-T., Park C.-J., Lee H.-W., Kim S.I., Chun J., Colwell R.R., Kim S.-J., Lee J.-H. J. Bacteriol. 190:7491-7499(2008) [PubMed: 18790866] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000855 Genomic DNA. Translation: ACJ17260.1.
RefSeq	YP_002308157.1. NC_011529.1.
3D structure databases
ProteinModelPortal	B6YV32.
ModBase	Search...
Protein-protein interaction databases
STRING	B6YV32.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7017439.
GenomeReviews	Gene locus TON_1770 in contig CP000855_GR.
KEGG	ton:TON_1770.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG351567.
OMA	DGAVDCH.
ProtClustDB	PRK13980.
Family and domain databases
HAMAP	MF_00193. NadE. [Tree]
InterPro	IPR022310. NAD/GMP_synthase. IPR003694. NAD_synthase. IPR022926. NH(3)-dep_NAD(+)_synth. IPR014729. Rossmann-like_a/b/a_fold. IPR018126. SASP_alpha/beta-type_CS. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01916.
Pfam	PF02540. NAD_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00552. NadE. 1 hit.
PROSITE	PS00304. SASP_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

B6YV32_THEON

Accession

Primary (citable) accession number: B6YV32

Entry history

Integrated into UniProtKB/TrEMBL:	January 20, 2009
Last sequence update:	January 20, 2009
Last modified:	December 14, 2011
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)