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B6YUZ1 (B6YUZ1_THEON) Unreviewed, UniProtKB/TrEMBL

Last modified January 25, 2012. Version 22. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Alanine--tRNA ligase HAMAP MF_00036
EC=6.1.1.7 HAMAP MF_00036
Alternative name(s):
Alanyl-tRNA synthetase HAMAP MF_00036
Gene names
Name:alaS HAMAP MF_00036
Ordered Locus Names:TON_1729
OrganismThermococcus onnurineus (strain NA1) [Complete proteome] [HAMAP]
Taxonomic identifier523850 [NCBI]
Taxonomic lineageArchaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Thermococcus

Protein attributes

Sequence length909 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editing domain By similarity. HAMAP MF_00036

Catalytic activity

ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala). HAMAP MF_00036 SAAS SAAS022429

Cofactor

Binds 1 zinc ion per subunit By similarity. HAMAP MF_00036 SAAS SAAS022429

Subcellular location

Cytoplasm By similarity HAMAP MF_00036.

Domain

Consists of three domains; the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs By similarity. HAMAP MF_00036

Sequence similarities

Belongs to the class-II aminoacyl-tRNA synthetase family. HAMAP MF_00036

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Sites

Metal binding6071Zinc By similarity HAMAP MF_00036
Metal binding6111Zinc By similarity HAMAP MF_00036
Metal binding7111Zinc By similarity HAMAP MF_00036
Metal binding7151Zinc By similarity HAMAP MF_00036

Sequences

Sequence LengthMass (Da)Tools
B6YUZ1 [UniParc].

Last modified January 20, 2009. Version 1.
Checksum: 06DFC6FAAE7688DF

FASTA909104,051
        10         20         30         40         50         60 
MSMDMTTRMF KEEGWMRKQC PKCGKFFWTL DPERKTCGDP PCDEYGFIGK PGIPKKYTLD 

        70         80         90        100        110        120 
EMREKFLSFF EKHGHGRVKR YPVLPRWRDD VLLVGASIMD FQPWVISGEA DPPANPLTIS 

       130        140        150        160        170        180 
QPSIRFTDID NVGITGRHFT IFEMMAHHAF NYPGKPIYWM DETVELAFEF FTKELGMKAE 

       190        200        210        220        230        240 
DITFKENPWA GGGNAGPAFE VLYRGLEVAT LVFMQYKKAP ENADPSQVVE IKGDKYVPME 

       250        260        270        280        290        300 
TMVVDTGYGL ERLVWMSQGT PTAYDAVLGY VIEPLKRMAG IEKIDERILM ENSKLAGMFD 

       310        320        330        340        350        360 
IEDMGDLRVL RQEVAKRVGI SVEELEKLVR PYELIYAIAD HTKALTFMLA DGVIPSNVKA 

       370        380        390        400        410        420 
GYLARLLIRK SIRHLRELGL EMPLAEIVAM HIKELSPSFP EFKEMEDVIL DIINVEEKRY 

       430        440        450        460        470        480 
QETLRRGSDL VKREIAKLKK KGENEIPLEK LILFYESHGL TPEIVAEIAQ KEGVKVHIPD 

       490        500        510        520        530        540 
NFYTLVAKEA EKEEKKEAAQ YVVDFELVKD LPDTRTLYYE DPFMKEFDAE VLRVIDDWVV 

       550        560        570        580        590        600 
LDRTAFYPEG GGQPYDTGEL NGVKVTNVQK VGKVILHRVE EPEKFKEGMT VHGRINWDRR 

       610        620        630        640        650        660 
IQHMRHHTGT HVLMGALVRV LGKHVWQAGS QLHTDWARLD ISHYKRITEE ELREIERLAN 

       670        680        690        700        710        720 
RIVMENRRVA WEWLPRTEAE MKYGFRLYQG GVVPGRIIRV LRIEDWDVQA CGGTHLPNTG 

       730        740        750        760        770        780 
LVGPIKILRT ERIQDGVERI IFAAGEAAVN WMQETERILK RTSEVFRVPP EKLPETAGRF 

       790        800        810        820        830        840 
FNEWKEARKE VERLRKELAK LLVYELEDRI ERVGEVEFIG AVVEGTMDDL REAANKLRKD 

       850        860        870        880        890        900 
KRVVLLISRE GHFVVAVGDG LDLKAGELAK VITSVAGGGG GGRKELAQGR IKNPLKAEEA 


IAEVKKRLG

« Hide

References

[1]"The complete genome sequence of Thermococcus onnurineus NA1 reveals a mixed heterotrophic and carboxydotrophic metabolism."
Lee H.S., Kang S.G., Bae S.S., Lim J.K., Cho Y., Kim Y.J., Jeon J.H., Cha S.-S., Kwon K.K., Kim H.-T., Park C.-J., Lee H.-W., Kim S.I., Chun J., Colwell R.R., Kim S.-J., Lee J.-H.
J. Bacteriol. 190:7491-7499(2008) [PubMed: 18790866] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000855 Genomic DNA. Translation: ACJ17219.1.
RefSeqYP_002308116.1. NC_011529.1.

3D structure databases

ProteinModelPortalB6YUZ1.
ModBaseSearch...

Protein-protein interaction databases

STRINGB6YUZ1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID7017398.
GenomeReviewsGene locus TON_1729 in contig CP000855_GR.
KEGGton:TON_1729.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG392147.
OMAMFTNSGM.
ProtClustDBPRK13902.

Family and domain databases

HAMAPMF_00036_A. Ala_tRNA_synth_A.
[Tree]
InterProIPR002318. Ala-tRNA-synth_IIc.
IPR018162. Ala-tRNA-synth_IIc_anticod-bd.
IPR018165. Ala-tRNA-synth_IIc_core.
IPR018164. Ala-tRNA-synth_IIc_N.
IPR022429. Ala-tRNA_synth_arc.
IPR003156. Pesterase_DHHA1.
IPR018163. Thr/Ala-tRNA-synth_IIc_edit.
IPR012947. tRNA_SAD.
[Graphical view]
KOK01872.
PANTHERPTHR11777:SF6. PTHR11777:SF6. 1 hit.
PfamPF02272. DHHA1. 1 hit.
PF01411. tRNA-synt_2c. 1 hit.
PF07973. tRNA_SAD. 1 hit.
[Graphical view]
PRINTSPR00980. TRNASYNTHALA.
SMARTSM00863. tRNA_SAD. 1 hit.
[Graphical view]
SUPFAMSSF101353. Ala-tRNA-synth_IIc_anticod-bd. 1 hit.
SSF55186. Thr/Ala-tRNA-synth_IIc_edit. 1 hit.
TIGRFAMsTIGR03683. A-tRNA_syn_arch. 1 hit.
TIGR00344. AlaS. 1 hit.
PROSITEPS50860. AA_TRNA_LIGASE_II_ALA. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameB6YUZ1_THEON
AccessionPrimary (citable) accession number: B6YUZ1
Entry history
Integrated into UniProtKB/TrEMBL: January 20, 2009
Last sequence update: January 20, 2009
Last modified: January 25, 2012
This is version 22 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info