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B6YUU0 (G3P_THEON) Reviewed, UniProtKB/Swiss-Prot

Last modified November 16, 2011. Version 29. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Glyceraldehyde-3-phosphate dehydrogenase
Short name=GAPDH
EC=1.2.1.59
Alternative name(s):
NAD(P)-dependent glyceraldehyde-3-phosphate dehydrogenase
Gene names
Name:gap
Ordered Locus Names:TON_0639
OrganismThermococcus onnurineus (strain NA1) [Complete proteome] [HAMAP]
Taxonomic identifier523850 [NCBI]
Taxonomic lineageArchaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Thermococcus

Protein attributes

Sequence length334 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

D-glyceraldehyde 3-phosphate + phosphate + NAD(P)+ = 3-phospho-D-glyceroyl phosphate + NAD(P)H. HAMAP MF_00559

Pathway

Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5. HAMAP MF_00559

Subunit structure

Homotetramer By similarity. HAMAP MF_00559

Subcellular location

Cytoplasm By similarity HAMAP MF_00559.

Sequence similarities

Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.

Ontologies

Keywords
   Biological processGlycolysis
   Cellular componentCytoplasm
   LigandNAD
NADP
   Molecular functionOxidoreductase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processglycolysis

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionNAD binding

Inferred from electronic annotation. Source: InterPro

NADP binding

Inferred from electronic annotation. Source: InterPro

glyceraldehyde-3-phosphate dehydrogenase (NAD(P)+) (phosphorylating) activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 334334Glyceraldehyde-3-phosphate dehydrogenase HAMAP MF_00559
PRO_1000129248

Regions

Nucleotide binding12 – 132NAD By similarity
Region140 – 1423Glyceraldehyde 3-phosphate binding By similarity
Region192 – 1932Glyceraldehyde 3-phosphate binding By similarity

Sites

Active site1411Nucleophile By similarity
Binding site1111NAD; via amide nitrogen By similarity
Binding site1671NAD By similarity
Binding site2981NAD; via carbonyl oxygen By similarity

Sequences

Sequence LengthMass (Da)Tools
B6YUU0 [UniParc].

Last modified January 20, 2009. Version 1.
Checksum: A58E52772425ACBF

FASTA33437,124
        10         20         30         40         50         60 
MKVKVGINGY GTIGKRVAYA VTRQDDMKLI GVTKTKPDFE AYRAKELGIP VYAAGEEFLP 

        70         80         90        100        110        120 
RFENAGFEVA GTLSDLLEKV DVIVDATPGG MGAKNKAVYE KAGVKAIFQG GEKASTAEVS 

       130        140        150        160        170        180 
FVAQANYEKA LGKDYVRVVS CNTTGLIRTL SAVQEYIDYV YAVMIRRAAD PNDIKHGPIN 

       190        200        210        220        230        240 
AIKPSVTVPS HHGPDVQTVI PINIETSAFV VPTTIMHVHS IMVELKKPLE AKDVIDIFEN 

       250        260        270        280        290        300 
TTRVLLFEKE KGFESTAQLI EFARDLHREW NNLYEIAVWK ESISVRGNRL FYIQAVHQES 

       310        320        330 
DVVPENIDAI RAMFEMADKW ESIRKTNQSL GILK

« Hide

References

[1]"The complete genome sequence of Thermococcus onnurineus NA1 reveals a mixed heterotrophic and carboxydotrophic metabolism."
Lee H.S., Kang S.G., Bae S.S., Lim J.K., Cho Y., Kim Y.J., Jeon J.H., Cha S.-S., Kwon K.K., Kim H.-T., Park C.-J., Lee H.-W., Kim S.I., Chun J., Colwell R.R., Kim S.-J., Lee J.-H.
J. Bacteriol. 190:7491-7499(2008) [PubMed: 18790866] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: NA1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000855 Genomic DNA. Translation: ACJ16126.1.
RefSeqYP_002307023.1. NC_011529.1.

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

STRINGB6YUU0.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID7016938.
GenomeReviewsGene locus TON_0639 in contig CP000855_GR.
KEGGton:TON_0639.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG392099.
OMAVPSHHGP.
ProtClustDBPRK04207.

Family and domain databases

HAMAPMF_00559. G3P_dehdrog_arch.
[Tree]
InterProIPR020831. GlycerAld/Erythrose_P_DH.
IPR020830. GlycerAld_3-P_DH_AS.
IPR020829. GlycerAld_3-P_DH_cat.
IPR020828. GlycerAld_3-P_DH_NAD(P)-bd.
IPR006436. Glyceraldehyde-3-P_DH_2_arc.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
Gene3DG3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KOK00150.
PfamPF02800. Gp_dh_C. 1 hit.
PF00044. Gp_dh_N. 1 hit.
[Graphical view]
PIRSFPIRSF000149. GAP_DH. 1 hit.
SMARTSM00846. Gp_dh_N. 1 hit.
[Graphical view]
TIGRFAMsTIGR01546. GAPDH-II_archae. 1 hit.
PROSITEPS00071. GAPDH. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameG3P_THEON
AccessionPrimary (citable) accession number: B6YUU0
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: January 20, 2009
Last modified: November 16, 2011
This is version 29 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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