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Protein

DNA ligase

Gene

lig

Organism
Thermococcus onnurineus (strain NA1)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair. Can use both ATP and NAD+, but NAD+ may be a preferred nucleotide cofactor.1 Publication

Catalytic activityi

ATP + (deoxyribonucleotide)(n)-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)(m) = (deoxyribonucleotide)(n+m) + AMP + diphosphate.UniRule annotation1 Publication
NAD+ + (deoxyribonucleotide)(n)-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)(m) = (deoxyribonucleotide)(n+m) + AMP + beta-nicotinamide D-nucleotide.UniRule annotation1 Publication

Cofactori

Mg2+1 Publication, Zn2+1 PublicationNote: Not stimulated by Ca2+, Mn2+ and Ni2+.1 Publication

pH dependencei

Optimum pH is 7.5.1 Publication

Temperature dependencei

Optimum temperature is 80 degrees Celsius.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei250ATPUniRule annotation1
Active sitei252N6-AMP-lysine intermediateUniRule annotation1
Binding sitei257ATPUniRule annotation1
Binding sitei272ATPUniRule annotation1
Binding sitei302ATPUniRule annotation1
Binding sitei342ATPUniRule annotation1
Binding sitei417ATPUniRule annotation1
Binding sitei423ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processCell cycle, Cell division, DNA damage, DNA recombination, DNA repair, DNA replication
LigandATP-binding, Magnesium, Metal-binding, NAD, Nucleotide-binding, Zinc

Enzyme and pathway databases

BRENDAi6.5.1.6 9350

Names & Taxonomyi

Protein namesi
Recommended name:
DNA ligase1 PublicationUniRule annotation (EC:6.5.1.6UniRule annotation1 Publication)
Alternative name(s):
Polydeoxyribonucleotide synthase [ATP/NAD(+)]UniRule annotationCurated
TNA1_lig1 Publication
Gene namesi
Name:ligUniRule annotation
Ordered Locus Names:TON_1515Imported
OrganismiThermococcus onnurineus (strain NA1)
Taxonomic identifieri523850 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaeThermococcus
Proteomesi
  • UP000002727 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004318401 – 562DNA ligaseAdd BLAST562

Interactioni

Protein-protein interaction databases

STRINGi523850.TON_1515

Structurei

3D structure databases

ProteinModelPortaliB6YTR4
SMRiB6YTR4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ATP-dependent DNA ligase family.UniRule annotationCurated

Phylogenomic databases

eggNOGiarCOG01347 Archaea
COG1793 LUCA
HOGENOMiHOG000036008
KOiK10747
OMAiWLFEESY
OrthoDBiPOG093Z03L0

Family and domain databases

Gene3Di1.10.3260.10, 1 hit
HAMAPiMF_00407 DNA_ligase, 1 hit
InterProiView protein in InterPro
IPR022865 DNA_ligae_ATP-dep_bac/arc
IPR000977 DNA_ligase_ATP-dep
IPR012309 DNA_ligase_ATP-dep_C
IPR012310 DNA_ligase_ATP-dep_cent
IPR016059 DNA_ligase_ATP-dep_CS
IPR012308 DNA_ligase_ATP-dep_N
IPR036599 DNA_ligase_N_sf
IPR012340 NA-bd_OB-fold
PfamiView protein in Pfam
PF04679 DNA_ligase_A_C, 1 hit
PF01068 DNA_ligase_A_M, 1 hit
PF04675 DNA_ligase_A_N, 1 hit
SUPFAMiSSF117018 SSF117018, 1 hit
SSF50249 SSF50249, 1 hit
TIGRFAMsiTIGR00574 dnl1, 1 hit
PROSITEiView protein in PROSITE
PS00697 DNA_LIGASE_A1, 1 hit
PS00333 DNA_LIGASE_A2, 1 hit
PS50160 DNA_LIGASE_A3, 1 hit

Sequencei

Sequence statusi: Complete.

B6YTR4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGDMKYTELS DLYRRLEKTT LKTLKTKFVA DFLKKTPDEL LEVVPYLILG
60 70 80 90 100
KVFPDWDERE LGVGEKLLIK AVSMATGVQE REIENSVKDT GDLGESVALA
110 120 130 140 150
LKKKKQKSFF SQPLTIKRVY QTFIKIAEAS GEGSQDRKLK YLANIFMDAQ
160 170 180 190 200
PEEGKYIART VLGMMRTGVA EGILRDAIAE AFKVKAELVE RAYMLTSDFG
210 220 230 240 250
YVAKVAKLEG NDGLGKVHIQ IGKPIRPMLA QNAASVKEAL LEMGAEAAFE
260 270 280 290 300
IKYDGARVQV HKDGDRVVIY SRRLENVTRS IPEVVDAIKA SIKSEKAIVE
310 320 330 340 350
GELVAVGEGG RPRPFQYVLR RFRRKYNIEE MIEKIPLELN LFDVLYVDGE
360 370 380 390 400
PLIDTPFRER RAKLEEIVEE GEKLKLAQQL VTKKVEEAEE FYKKALELGH
410 420 430 440 450
EGLMAKRLDS VYEPGNRGKK WLKIKPTMED LDLVIIGAEW GEGRRAHLLG
460 470 480 490 500
SFLVAAYDPH SGEFVPVGKV GSGFTDEDLV EFTKMLKPLI IGGEGKFVEI
510 520 530 540 550
EPKVVIQVTY QEIQKSPKYR SGFALRFPRY VALREDKSPE EADTIERIAQ
560
LYEFQERFKA KK
Length:562
Mass (Da):63,655
Last modified:January 20, 2009 - v1
Checksum:iA54BDEB51585437D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ223722 Genomic DNA Translation: ABC11973.1
CP000855 Genomic DNA Translation: ACJ17005.1

Genome annotation databases

EnsemblBacteriaiACJ17005; ACJ17005; TON_1515
KEGGiton:TON_1515
PATRICifig|523850.10.peg.1528

Similar proteinsi

Entry informationi

Entry nameiDNLI_THEON
AccessioniPrimary (citable) accession number: B6YTR4
Secondary accession number(s): Q2Q452
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 4, 2015
Last sequence update: January 20, 2009
Last modified: March 28, 2018
This is version 62 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health