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Protein

Methionine aminopeptidase

Gene

map

Organism
Thermococcus onnurineus (strain NA1)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val).UniRule annotation1 Publication

Catalytic activityi

Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.UniRule annotation1 Publication

Cofactori

Fe2+By similarity1 Publication, Co2+By similarity1 Publication, Ni2+By similarity1 Publication, Mn2+By similarity1 PublicationNote: Binds 2 divalent metal cations per subunit. Has a high-affinity and a low affinity metal-binding site. The true nature of the physiological cofactor is under debate. Most likely, methionine aminopeptidases function as mononuclear Fe2+-metalloproteases under physiological conditions, and the catalytically relevant metal-binding site has been assigned to the histidine-containing high-affinity site. The enzyme is active with cobalt, nickel, manganese and divalent iron ions.By similarity1 Publication

Kineticsi

kcat is 168 min(-1) with L-Met-p-nitroanilide as substrate.
  1. KM=0.68 mM for L-Met-p-nitroanilide1 Publication

    pH dependencei

    Optimum pH is 7.1 Publication

    Temperature dependencei

    Optimum temperature is 80-90 degrees Celsius.1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei63SubstrateUniRule annotation1
    Metal bindingi83Divalent metal cation 1UniRule annotation1
    Metal bindingi94Divalent metal cation 1UniRule annotation1
    Metal bindingi94Divalent metal cation 2; catalyticUniRule annotation1
    Metal bindingi154Divalent metal cation 2; catalytic; via tele nitrogenUniRule annotation1
    Binding sitei162SubstrateUniRule annotation1
    Metal bindingi188Divalent metal cation 2; catalyticUniRule annotation1
    Metal bindingi281Divalent metal cation 1UniRule annotation1
    Metal bindingi281Divalent metal cation 2; catalyticUniRule annotation1

    GO - Molecular functioni

    Keywordsi

    Molecular functionAminopeptidase, Hydrolase, Protease
    LigandMetal-binding

    Enzyme and pathway databases

    BioCyciTONN523850:G1GAB-386-MONOMER

    Protein family/group databases

    MEROPSiM24.035

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Methionine aminopeptidaseUniRule annotation (EC:3.4.11.18UniRule annotation)
    Short name:
    MAPUniRule annotation
    Short name:
    MetAPUniRule annotation
    Alternative name(s):
    Peptidase MUniRule annotation
    Gene namesi
    Name:mapUniRule annotation
    Ordered Locus Names:TON_0362
    OrganismiThermococcus onnurineus (strain NA1)
    Taxonomic identifieri523850 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaeThermococcus
    Proteomesi
    • UP000002727 Componenti: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00004288261 – 295Methionine aminopeptidaseAdd BLAST295

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi523850.TON_0362

    Structurei

    3D structure databases

    ProteinModelPortaliB6YTG0
    SMRiB6YTG0
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the peptidase M24A family. Methionine aminopeptidase archaeal type 2 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiarCOG01001 Archaea
    COG0024 LUCA
    HOGENOMiHOG000226277
    KOiK01265
    OMAiHTVLLMP
    OrthoDBiPOG093Z06FJ

    Family and domain databases

    CDDicd01088 MetAP2, 1 hit
    Gene3Di1.10.10.10, 1 hit
    HAMAPiMF_01975 MetAP_2_arc, 1 hit
    InterProiView protein in InterPro
    IPR036005 Creatinase/aminopeptidase-like
    IPR028595 MetAP_archaeal
    IPR000994 Pept_M24
    IPR001714 Pept_M24_MAP
    IPR002468 Pept_M24A_MAP2
    IPR018349 Pept_M24A_MAP2_BS
    IPR036388 WH-like_DNA-bd_sf
    IPR036390 WH_DNA-bd_sf
    PfamiView protein in Pfam
    PF00557 Peptidase_M24, 1 hit
    PRINTSiPR00599 MAPEPTIDASE
    SUPFAMiSSF46785 SSF46785, 1 hit
    SSF55920 SSF55920, 2 hits
    TIGRFAMsiTIGR00501 met_pdase_II, 1 hit
    PROSITEiView protein in PROSITE
    PS01202 MAP_2, 1 hit

    Sequencei

    Sequence statusi: Complete.

    B6YTG0-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MDEREALIKA GEIARQVKKE VISLIKPGTK LYDIAEFVER RIIELGGKPA
    60 70 80 90 100
    FPCNLSINEI AAHYTPYKGD ETVLKEGDYL KVDIGVHVDG YIADTALTFR
    110 120 130 140 150
    VGMEEDDLVT AAREALENAI KVIRAGIKIN EIGKAIEETI RGYGFNPIVN
    160 170 180 190 200
    LSGHKIERYK LHAGISIPNI YRPADSYVLK EGDVIAIEPF ATTGAGQVIE
    210 220 230 240 250
    VPPALIFMYL RDRPVRMAQA RRVLMHIKRE YNGLPFAYRW LQGFMPEGQL
    260 270 280 290
    KLALAQLDRV GAIYSYPILR EVRGGLVAQF EHTVIVEKEG AYITT
    Length:295
    Mass (Da):33,013
    Last modified:January 20, 2009 - v1
    Checksum:i743CE50ECD2622D2
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    DQ144133 Genomic DNA Translation: ABA26945.1
    CP000855 Genomic DNA Translation: ACJ15847.1
    RefSeqiWP_012571319.1, NC_011529.1

    Genome annotation databases

    EnsemblBacteriaiACJ15847; ACJ15847; TON_0362
    GeneIDi7018028
    KEGGiton:TON_0362
    PATRICifig|523850.10.peg.365

    Similar proteinsi

    Entry informationi

    Entry nameiMAP2_THEON
    AccessioniPrimary (citable) accession number: B6YTG0
    Secondary accession number(s): Q2QC91
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 16, 2014
    Last sequence update: January 20, 2009
    Last modified: March 28, 2018
    This is version 66 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome
    UniProt is an ELIXIR core data resource
    Main funding by: National Institutes of Health