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B6YST9 (SYL_THEON) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 40. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:TON_0141
OrganismThermococcus onnurineus (strain NA1) [Complete proteome] [HAMAP]
Taxonomic identifier523850 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaeThermococcus

Protein attributes

Sequence length967 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 967967Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000199235

Regions

Motif43 – 5311"HIGH" region HAMAP-Rule MF_00049
Motif650 – 6545"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6531ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
B6YST9 [UniParc].

Last modified January 20, 2009. Version 1.
Checksum: BC2818D12683C5ED

FASTA967113,529
        10         20         30         40         50         60 
MAELNFKAIE EKWQKRWMED RVFEPDRNAK PKEKKFYITV AFPYLSGHLH VGHARTYTIP 

        70         80         90        100        110        120 
DVIARFKRMQ GYNVLFPMAW HITGAPIVGI AERIKNRDPK TIHIYRDVYK VPEEILWKFE 

       130        140        150        160        170        180 
DPKEIVKYFM KAARETFIRA GFSVDWSREF HTTSLFPPFS KFIEWQFWTL KDMGLVVKGA 

       190        200        210        220        230        240 
HRVRWDPVVG TPLGDHDIME GEDVQILEYV IIKFILEENG EEIYMPAATL RPETVYGVTN 

       250        260        270        280        290        300 
MWLNPEAIYV KAKVRRGDRE ETWIISKEAA YKLSFQDREI EVIEEFKGER LIGKYVKNPV 

       310        320        330        340        350        360 
TGDEVIILPA EFVDPDNATG VVMSVPAHAP FDHVALEDLK KETEILLKYD IDPRVVEEIS 

       370        380        390        400        410        420 
YISLIKLEGY GEFPAVEEVE KLGVKSQKDE EKLEEATKNI YKAEYHKGVF KIEPYAGKPV 

       430        440        450        460        470        480 
QEVKDLIAKE LQEKGIAEIM YEFAEKPVIS RFGNQAVIKI IHDQWFIDYG NPEWKEKARE 

       490        500        510        520        530        540 
ALANMTIYPE SRRTQFEAVI DWLDKKACAR KVGLGTPLPW DPEWVIESLS DSTIYMAYYT 

       550        560        570        580        590        600 
ISRHINKLRE EGRLDPEKLD REFFDYLFRE EFSEEREKEL AEKTGIPAEI IHEMKEEFEY 

       610        620        630        640        650        660 
WYPLDWRCSA KDLIPNHLTF FIFNHVAIFR KGHWPRGIAV NGFGTLEGQK MSKSKGNVLN 

       670        680        690        700        710        720 
FIDAIEENGA DVVRLYIMGL AEHDSDFDWR RKEVGKLRKQ VERFYELVSE FASYEAKEGV 

       730        740        750        760        770        780 
ELKDIDRWML HRLNKAIEGA TKGLEEFRTR TAVQWAFYSV LNDLRWYLRR TEGRDDEAKR 

       790        800        810        820        830        840 
YVLRTLADVW VRLMAPFTPH ISEELWEKLG GEGFVSLAKW PEPNPAWWNE TIELEEEYVK 

       850        860        870        880        890        900 
NLIEDIKEII RVAKIEDAKR AYIYTAPEWK WRVAEAVAEK RDFKAAMSEL MKDPEMRKHG 

       910        920        930        940        950        960 
KEISKMIQRL IKDRAFEIKR IDEEKALREA KDFIEKELGL EIIINPEEDK GGKKKAAMPM 


KPAVFVE 

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References

[1]"The complete genome sequence of Thermococcus onnurineus NA1 reveals a mixed heterotrophic and carboxydotrophic metabolism."
Lee H.S., Kang S.G., Bae S.S., Lim J.K., Cho Y., Kim Y.J., Jeon J.H., Cha S.-S., Kwon K.K., Kim H.-T., Park C.-J., Lee H.-W., Kim S.I., Chun J., Colwell R.R., Kim S.-J., Lee J.-H.
J. Bacteriol. 190:7491-7499(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: NA1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000855 Genomic DNA. Translation: ACJ15626.1.
RefSeqYP_002306523.1. NC_011529.1.

3D structure databases

ProteinModelPortalB6YST9.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING523850.TON_0141.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACJ15626; ACJ15626; TON_0141.
GeneID7017795.
KEGGton:TON_0141.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000216620.
KOK01869.
OMAYSKFVEW.

Enzyme and pathway databases

BioCycTONN523850:GC7X-150-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_A. Leu_tRNA_synth_A.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR020791. Leu-tRNA-lgase_arc.
IPR004493. Leu-tRNA-synth_Ia_arc/euk.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 2 hits.
TIGRFAMsTIGR00395. leuS_arch. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_THEON
AccessionPrimary (citable) accession number: B6YST9
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: January 20, 2009
Last modified: May 14, 2014
This is version 40 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries