Reviewed, UniProtKB/Swiss-Prot B6YSJ5 (RTCA_THEON)

Names and origin

Protein names

Recommended name:
    Probable RNA 3'-terminal phosphate cyclase
      Short name=RNA-3'-phosphate cyclase
      Short name=RNA cyclase
    EC=6.5.1.4

Gene names

Name:	rtcA
Ordered Locus Names:	TON_0048

Organism

Thermococcus onnurineus (strain NA1) [Complete proteome] [HAMAP]

Taxonomic identifier

523850 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Thermococcus

Protein attributes

Sequence length	340 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) the enzyme acts on RNA-N3'P to produce RNA-N3'PP5'A; (C) a non catalytic nucleophilic attack by the adjacent 2'hydroxyl on the phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing By similarity. HAMAP MF_00200
Catalytic activity	ATP + RNA 3'-terminal-phosphate = AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate. HAMAP MF_00200
Subcellular location	Cytoplasm Potential HAMAP MF_00200.
Sequence similarities	Belongs to the RNA 3'-terminal cyclase family. Type 1 subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	RNA processing Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA-3'-phosphate cyclase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 340	340	Probable RNA 3'-terminal phosphate cyclase HAMAP MF_00200		PRO_1000099357
Sites
	Active site	308	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	B6YSJ5-1 [UniParc]. Last modified January 20, 2009. Version 1. Checksum: 17E27D4184680FC1	FASTA	340	36,946
	10 20 30 40 50 60 MSMIVIDGSY GEGGGQILRT SVALSVITGK PVRIYNIRAN RPNPGLRPQH MHGILALKEL 70 80 90 100 110 120 SGAKVKGAQV GSTVLEFYPG RARPRHIRVP IKTAGSVTLV LQALLPAMAF IGGSFEITGG 130 140 150 160 170 180 TDVPWSPPVD YLRHVTLFAL EKMGLRAEIE IKRRGHYPKG GGLVVGRVEP WEERKPLVAL 190 200 210 220 230 240 EWRHIELFGG ISHATNLPEH VASRQAKAAR ERLSEFYDVP INIYEEVSRS LGPGSGIVVW 250 260 270 280 290 300 AETDVLRLGG DALGKRGKPA EAVGREAADE LLEQLTSRAA VDRFLGDQLV PFLAFAGGEI 310 320 330 340 KVAEITNHLV TNVWVVEQFL GKIFEVEGEV GEPGRVRVVG

References

[1]

"The complete genome sequence of Thermococcus onnurineus NA1 reveals a mixed heterotrophic and carboxydotrophic metabolism." Lee H.S., Kang S.G., Bae S.S., Lim J.K., Cho Y., Kim Y.J., Jeon J.H., Cha S.-S., Kwon K.K., Kim H.-T., Park C.-J., Lee H.-W., Kim S.I., Chun J., Colwell R.R., Kim S.-J., Lee J.-H. J. Bacteriol. 190:7491-7499(2008) [PubMed: 18790866] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000855 Genomic DNA. Translation: ACJ15532.1.
RefSeq	YP_002306429.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	7017694.
GenomeReviews	Gene locus TON_0048 in contig CP000855_GR.
KEGG	ton:TON_0048.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG683539.
OMA	EIDGSYG.
Family and domain databases
HAMAP	MF_00200. RTC. [Tree]
InterPro	IPR013791. RNA3'-term_phos_cycl_insert. IPR000228. RNA3'_term_phos_cycl-like. IPR020719. RNA3'_term_phos_cycl-like_CS. IPR013796. RNA3'_term_phos_cycl_insert. IPR020723. RNA3'_term_phos_cycl_prd. IPR017770. RNA3'_term_phos_cycl_sub. IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b. [Graphical view]
Gene3D	G3DSA:3.65.10.20. RNA3'_term_phos_cycl. 1 hit.
PANTHER	PTHR11096. RNA3'_term_phos_cycl. 1 hit.
Pfam	PF01137. RTC. 1 hit. PF05189. RTC_insert. 1 hit. [Graphical view]
TIGRFAMs	TIGR03399. RNA_3prim_cycl. 1 hit.
PROSITE	PS01287. RTC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RTCA_THEON

Accession

Primary (citable) accession number: B6YSJ5

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	January 20, 2009
Last modified:	February 9, 2010
This is version 14 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)