Reviewed, UniProtKB/Swiss-Prot B6YS29 (MRAY_AZOPC)

Names and origin

Protein names

Recommended name:
    Phospho-N-acetylmuramoyl-pentapeptide-transferase
    EC=2.7.8.13
Alternative name(s):
    UDP-MurNAc-pentapeptide phosphotransferase

Gene names

Name:	mraY
Ordered Locus Names:	CFPG_738

Organism

Azobacteroides pseudotrichonymphae genomovar. CFP2 [Complete proteome] [HAMAP]

Taxonomic identifier

511995 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Bacteroidia › Bacteroidales › Candidatus Azobacteroides

Protein attributes

Sequence length	418 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	First step of the lipid cycle reactions in the biosynthesis of the cell wall peptidoglycan By similarity.
Catalytic activity	UDP-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala) + undecaprenyl phosphate = UMP + Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol. HAMAP MF_00038
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00038
Subcellular location	Cell inner membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the glycosyltransferase 4 family. MraY subfamily.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: HAMAP
Molecular function	phospho-N-acetylmuramoyl-pentapeptide-transferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 418	418	Phospho-N-acetylmuramoyl-pentapeptide-transferase HAMAP MF_00038		PRO_1000090590
Regions
	Transmembrane	22 – 42	21	Potential
	Transmembrane	72 – 92	21	Potential
	Transmembrane	95 – 115	21	Potential
	Transmembrane	135 – 155	21	Potential
	Transmembrane	208 – 228	21	Potential
	Transmembrane	244 – 264	21	Potential
	Transmembrane	277 – 297	21	Potential
	Transmembrane	302 – 322	21	Potential
	Transmembrane	326 – 346	21	Potential
	Transmembrane	395 – 415	21	Potential

Sequences

	Sequence		Length	Mass (Da)
	B6YS29-1 [UniParc]. Last modified January 20, 2009. Version 1. Checksum: 30F2E0263072F9B1	FASTA	418	46,028
	10 20 30 40 50 60 MLYSLFYFLS SLNVPGARLF RYISFRSVLS FSLSLIISVL IGERIIRFLQ RHQAGETIRN 70 80 90 100 110 120 LGLEGQIQKK GTPTMGGIII IIAILIPVLL LARLSNIYVL LMLITTIWLG ILGLIDDCIK 130 140 150 160 170 180 VIWKHKEGLS GKIKIIAQVG LGLIVGLIVY LSPNIVMYEN VEIRQQGGRK TVSYTPPLKS 190 200 210 220 230 240 TQTTVPFFKN NNLDYRDLTA FMTNHRTAAT WILFVLITVF IVTAVSNGAN LTDGLDGLTA 250 260 270 280 290 300 GSSAIIGVVL GILAYVSANL GFAAYLNIMY IPGCGELTVF ASAFVGACMG FLWHNAFPAQ 310 320 330 340 350 360 VFMGDIGSLT LGGIIAVFAI IIHKELLLPM LCGIFFTESL SVMIQVAYFK YSKRKTGMGK 370 380 390 400 410 RIFKMTPIHH HFQKGGSEEV NVIIQKPLNA IPESKIVVRF WLIGILLAAL TVVTLKIR

References

[1]

"Genome of an endosymbiont coupling N2 fixation to cellulolysis within RT protist cells in termite gut." Hongoh Y., Sharma V.K., Prakash T., Noda S., Toh H., Taylor T.D., Kudo T., Sakaki Y., Toyoda A., Hattori M., Ohkuma M. Science 322:1108-1109(2008) [PubMed: 19008447] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AP010656 Genomic DNA. Translation: BAG84001.1.
RefSeq	YP_002309412.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	7039993.
GenomeReviews	Gene locus CFPG_738 in contig AP010656_GR.
KEGG	aps:CFPG_738.
Organism-specific databases
CMR	Search...
Family and domain databases
HAMAP	MF_00038. [Tree]
InterPro	IPR000715. Glycosyl_transferase_4. IPR018481. Glycosyl_Trfase_4_cons-reg. IPR003524. PNAcMuramoyl-5peptid_Trfase. IPR018480. PNAcMuramoyl-5peptid_Trfase_CS. [Graphical view]
PANTHER	PTHR22926. Glyco_trans_4. 1 hit. PTHR22926:SF3. PNAcPpept_trans. 1 hit.
Pfam	PF00953. Glycos_transf_4. 1 hit. PF10555. MraY_sig1. 1 hit. [Graphical view]
TIGRFAMs	TIGR00445. mraY. 1 hit.
PROSITE	PS01347. MRAY_1. 1 hit. PS01348. MRAY_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MRAY_AZOPC

Accession

Primary (citable) accession number: B6YS29

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	January 20, 2009
Last modified:	October 13, 2009
This is version 9 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)