Reviewed, UniProtKB/Swiss-Prot B6YQV1 (ILVD_AZOPC)

Names and origin

Protein names

Recommended name:
    Dihydroxy-acid dehydratase
      Short name=DAD
    EC=4.2.1.9

Gene names

Name:	ilvD
Ordered Locus Names:	CFPG_310

Organism

Azobacteroides pseudotrichonymphae genomovar. CFP2 [Complete proteome] [HAMAP]

Taxonomic identifier

511995 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Bacteroidia › Bacteroidales › Candidatus Azobacteroides

Protein attributes

Sequence length	609 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O. HAMAP MF_00012
Cofactor	Binds 1 4Fe-4S cluster Potential.
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4. HAMAP MF_00012 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4. HAMAP MF_00012
Sequence similarities	Belongs to the ilvD/edd family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoleucine biosynthetic process Inferred from electronic annotation. Source: HAMAP valine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW dihydroxy-acid dehydratase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 609	609	Dihydroxy-acid dehydratase HAMAP MF_00012		PRO_1000089367
Sites
	Metal binding	123	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	192	1	Iron-sulfur (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	B6YQV1-1 [UniParc]. Last modified January 20, 2009. Version 1. Checksum: 0ED33A3F6451DF14	FASTA	609	65,889
	10 20 30 40 50 60 MKIQLRSHES TYGRKMAGTR ALWKANGMKV EQFNKPIIAI VNSFTQFVPG HAHLHEVGQF 70 80 90 100 110 120 VKSEIEKQGC FAAEFNTIAI DDGIAMGHNG MLYSLPSRDL IADSVEYMIQ AHKADAMVCI 130 140 150 160 170 180 SNCDKITPGM LMASMRLNIP TVFVSGGPME AGNLNGNRLD LIEVMVQSAD AFVSDEQVRQ 190 200 210 220 230 240 VELNACPTCG SCSGMFTANS MNCLNEAIGL ALPGNGTIVA THTRRKQLFA DAASLIVKNT 250 260 270 280 290 300 YKYYEKGNEN VLPLSIATRE SFLNAMTLDI AMGGSTNTIL HLLAIAHEAG VDFTMQDIDT 310 320 330 340 350 360 LSRKTPCLCK ISPNASKYYI QDVNRAGGII GILSELHKSK LIDVSAKRVD GLTVAEAIRQ 370 380 390 400 410 420 FDICSTDVTK EALNKYKSAP GNRFNLVMAS QQNDYDTLDT DRMNGCIRST KYAYSEDGGL 430 440 450 460 470 480 AILKGNIAMD GCVVKTAGID ENIFLFSGPA KVFDSQESAC RGILEGKIVS GDVVVIIYEG 490 500 510 520 530 540 PKGGPGMQEM LYPTSYIKSR HLGKGCALIT DGRFSGGTSG LSIGHISPEA AASGNIGLVR 550 560 570 580 590 600 NNDIIAINIP ERTINVLLSE EELAKRRKEE LQRGDAAFTP PNRKRIVPKT LRAYASMVSS ADKGAVRII

References

[1]

"Genome of an endosymbiont coupling N2 fixation to cellulolysis within RT protist cells in termite gut." Hongoh Y., Sharma V.K., Prakash T., Noda S., Toh H., Taylor T.D., Kudo T., Sakaki Y., Toyoda A., Hattori M., Ohkuma M. Science 322:1108-1109(2008) [PubMed: 19008447] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AP010656 Genomic DNA. Translation: BAG83573.1.
RefSeq	YP_002308984.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	7039258.
GenomeReviews	Gene locus CFPG_310 in contig AP010656_GR.
KEGG	aps:CFPG_310.
Organism-specific databases
CMR	Search...
Family and domain databases
HAMAP	MF_00012. [Tree]
InterPro	IPR004404. DihydroxyA_deHydtase. IPR000581. DiOHA_6PGluconate_deHydtase. IPR020558. DiOHA_6PGluconate_deHydtase_CS. [Graphical view]
PANTHER	PTHR21000. ILVD_EDD_family. 1 hit.
Pfam	PF00920. ILVD_EDD. 1 hit. [Graphical view]
TIGRFAMs	TIGR00110. ilvD. 1 hit.
PROSITE	PS00886. ILVD_EDD_1. 1 hit. PS00887. ILVD_EDD_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ILVD_AZOPC

Accession

Primary (citable) accession number: B6YQV1

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	January 20, 2009
Last modified:	October 13, 2009
This is version 9 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)