B6YQU1 (SYC_AZOPC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cysteine--tRNA ligase
EC=6.1.1.16

Alternative name(s):
Cysteinyl-tRNA synthetase
Short name=CysRS

Gene names

Name:	cysS
Ordered Locus Names:	CFPG_300

Organism

Azobacteroides pseudotrichonymphae genomovar. CFP2 [Complete proteome] [HAMAP]

Taxonomic identifier

511995 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Bacteroidia › Bacteroidales › Candidatus Azobacteroides

Protein attributes

Sequence length	492 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys). HAMAP MF_00041
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00041
Subunit structure	Monomer By similarity. HAMAP MF_00041
Subcellular location	Cytoplasm By similarity HAMAP MF_00041.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cysteinyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW cysteine-tRNA ligase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 492	492	Cysteine--tRNA ligase HAMAP MF_00041		PRO_1000090816
Regions
	Motif	33 – 43	11	"HIGH" region HAMAP MF_00041
	Motif	283 – 287	5	"KMSKS" region HAMAP MF_00041
Sites
	Metal binding	31	1	Zinc By similarity
	Metal binding	226	1	Zinc By similarity
	Metal binding	251	1	Zinc By similarity
	Metal binding	255	1	Zinc By similarity
	Binding site	286	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	B6YQU1 [UniParc]. Last modified January 20, 2009. Version 1. Checksum: 762FBEDB8DA9B972	FASTA	492	56,682
	10 20 30 40 50 60 MKNQLFIYNT LTGRKELFQS LYPKRVGLYV CGPTVYGDPH LGHARPAITF DILFRYLMHL 70 80 90 100 110 120 NYKVRYVRNI TDVGHLTSDS DLGEDKIARK ARLEDLEPME VVQHYLNLYH KTMDALNVLP 130 140 150 160 170 180 PSIEPHASAH IIEQIQLIKE ILEKGYAYES KGSVYFDVEK YNKKYNYGKL SGQNIADMLN 190 200 210 220 230 240 TTRKLDGQEG KRNPIDFALW KKASSKHIMQ WISPWSNGFP GWHLECTTMS RKYLGNLFDI 250 260 270 280 290 300 HGGGMDLIFP HHECEIAQKV ASTGYEGVKY WMHNNMVTVN GQKMGKSSNN FINLEQLFNG 310 320 330 340 350 360 TNPLLIQSYN PMTVRFFILQ SHYRNTIDFS NKALQASKKG LSRLLEANNN IKQLTAQTTN 370 380 390 400 410 420 STVNIEGLRN KSIEAMNDDL NTPIIISYLF EATRIVNSAL AKQTQLTTED IQQLKDFFQL 430 440 450 460 470 480 FLFNLLGIKD ELKYKNTSYN SFAKAVDLLL QIRVQAKQEK NWIFADKIRD ELTVLGFEVK 490 DTKNGFEWKL SK

References

[1]

"Genome of an endosymbiont coupling N2 fixation to cellulolysis within RT protist cells in termite gut." Hongoh Y., Sharma V.K., Prakash T., Noda S., Toh H., Taylor T.D., Kudo T., Sakaki Y., Toyoda A., Hattori M., Ohkuma M. Science 322:1108-1109(2008) [PubMed: 19008447] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP010656 Genomic DNA. Translation: BAG83563.1.
RefSeq	YP_002308974.1. NC_011565.1.
3D structure databases
ProteinModelPortal	B6YQU1.
ModBase	Search...
Protein-protein interaction databases
STRING	B6YQU1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7039294.
GenomeReviews	Gene locus CFPG_300 in contig AP010656_GR.
KEGG	aps:CFPG_300.
PATRIC	31962267. VBICanAzo57536_0509.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG327651.
OMA	NVFDIHG.
ProtClustDB	PRK00260.
Family and domain databases
HAMAP	MF_00041. Cys_tRNA_synth. [Tree]
InterPro	IPR015803. Cys-tRNA-synt. IPR015273. Cys-tRNA-synt_Ia_DALR. IPR024909. Cys-tRNA/MSH_ligase. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits.
KO	K01883.
PANTHER	PTHR10890. Cys_tRNA-synt_1a. 1 hit.
Pfam	PF09190. DALR_2. 1 hit. PF01406. tRNA-synt_1e. 1 hit. [Graphical view]
PRINTS	PR00983. TRNASYNTHCYS.
SMART	SM00840. DALR_2. 1 hit. [Graphical view]
SUPFAM	SSF47323. tRNAsyn_1a_bind. 1 hit.
TIGRFAMs	TIGR00435. CysS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYC_AZOPC

Accession

Primary (citable) accession number: B6YQU1

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	January 20, 2009
Last modified:	January 25, 2012
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program