Reviewed, UniProtKB/Swiss-Prot B6YQC6 (ISPE_AZOPC)

Names and origin

Protein names

Recommended name:
    4-diphosphocytidyl-2-C-methyl-D-erythritol kinase
      Short name=CMK
    EC=2.7.1.148
Alternative name(s):
    4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Gene names

Name:	ispE
Ordered Locus Names:	CFPG_135

Organism

Azobacteroides pseudotrichonymphae genomovar. CFP2 [Complete proteome] [HAMAP]

Taxonomic identifier

511995 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Bacteroidia › Bacteroidales › Candidatus Azobacteroides

Protein attributes

Sequence length	267 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol By similarity.
Catalytic activity	ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00061
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 3/6. HAMAP MF_00061
Sequence similarities	Belongs to the GHMP kinase family. IspE subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phosphorylation Inferred from electronic annotation. Source: InterPro terpenoid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase activity Inferred from electronic annotation. Source: HAMAP ATP binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 267	267	4-diphosphocytidyl-2-C-methyl-D-erythritol kinase HAMAP MF_00061		PRO_1000092061
Regions
	Nucleotide binding	90 – 100	11	ATP Potential
Sites
	Active site	8	1	By similarity
	Active site	132	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	B6YQC6-1 [UniParc]. Last modified January 20, 2009. Version 1. Checksum: 579B93052DCC4D08	FASTA	267	30,042
	10 20 30 40 50 60 MIAFPNAKIN LGLHVIARRS DGYHDIETIF CPIDLCDILE IVSATQTKFI QSGIPMDCKV 70 80 90 100 110 120 SNNLVMKAYQ LLKKHYNLPE ITIHLRKNIP IGAGLGGGSS DASFMLYLLS NLANLCLSIE 130 140 150 160 170 180 ELEAYAKQLG SDCPFFIRNK PVFAKGIGNI FTPIELDLKK YNLALVTTNI HISTSEAYTN 190 200 210 220 230 240 IVVSHTPRES LTDIIHYPVD KWKNRLVNDF EEDIFPLYPE IREIKQELYN QGAIYASMSG 250 260 SGSSVYGLFK KPYRPIGTFN FSIHNFI

References

[1]

"Genome of an endosymbiont coupling N2 fixation to cellulolysis within RT protist cells in termite gut." Hongoh Y., Sharma V.K., Prakash T., Noda S., Toh H., Taylor T.D., Kudo T., Sakaki Y., Toyoda A., Hattori M., Ohkuma M. Science 322:1108-1109(2008) [PubMed: 19008447] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AP010656 Genomic DNA. Translation: BAG83398.1.
RefSeq	YP_002308809.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	7039482.
GenomeReviews	Gene locus CFPG_135 in contig AP010656_GR.
KEGG	aps:CFPG_135.
Organism-specific databases
CMR	Search...
Family and domain databases
HAMAP	MF_00061. [Tree]
InterPro	IPR006204. GHMP_kinase. IPR013750. GHMP_kinase_C. IPR004424. IspE. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
PANTHER	PTHR20861:SF2. IspE. 1 hit.
Pfam	PF08544. GHMP_kinases_C. 1 hit. PF00288. GHMP_kinases_N. 1 hit. [Graphical view]
PIRSF	PIRSF010376. IspE. 1 hit.
TIGRFAMs	TIGR00154. ispE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ISPE_AZOPC

Accession

Primary (citable) accession number: B6YQC6

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	January 20, 2009
Last modified:	September 22, 2009
This is version 8 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)