ID   B6VLS7_PHOAA            Unreviewed;       878 AA.
AC   B6VLS7; C7BRB8;
DT   16-DEC-2008, integrated into UniProtKB/TrEMBL.
DT   16-DEC-2008, sequence version 1.
DT   27-MAR-2024, entry version 89.
DE   RecName: Full=Phosphoenolpyruvate carboxylase {ECO:0000256|ARBA:ARBA00022419, ECO:0000256|HAMAP-Rule:MF_00595};
DE            Short=PEPC {ECO:0000256|HAMAP-Rule:MF_00595};
DE            Short=PEPCase {ECO:0000256|HAMAP-Rule:MF_00595};
DE            EC=4.1.1.31 {ECO:0000256|ARBA:ARBA00012305, ECO:0000256|HAMAP-Rule:MF_00595};
GN   Name=ppc {ECO:0000256|HAMAP-Rule:MF_00595,
GN   ECO:0000313|EMBL:CAR67107.1};
GN   OrderedLocusNames=PAU_04239 {ECO:0000313|EMBL:CAQ86326.1};
GN   ORFNames=PA-RVA9-1866 {ECO:0000313|EMBL:CAR67107.1};
OS   Photorhabdus asymbiotica subsp. asymbiotica (strain ATCC 43949 / 3105-77)
OS   (Xenorhabdus luminescens (strain 2)).
OC   Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
OC   Morganellaceae; Photorhabdus.
OX   NCBI_TaxID=553480 {ECO:0000313|EMBL:CAR67107.1};
RN   [1] {ECO:0000313|EMBL:CAR67107.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=ATCC 43949 {ECO:0000313|EMBL:CAR67107.1};
RX   PubMed=18838673; DOI=10.1073/pnas.0711114105;
RA   Waterfield N.R., Sanchez-Contreras M., Eleftherianos I., Dowling A.,
RA   Wilkinson P., Parkhill J., Thomson N., Reynolds S.E., Bode H.B., Dorus S.,
RA   Ffrench-Constant R.H.;
RT   "Rapid virulence annotation (RVA): identification of virulence factors
RT   using a bacterial genome library and multiple invertebrate hosts.";
RL   Proc. Natl. Acad. Sci. U.S.A. 105:15967-15972(2008).
RN   [2] {ECO:0000313|EMBL:CAQ86326.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=ATCC43949 {ECO:0000313|EMBL:CAQ86326.1};
RA   Crossman L.C.;
RL   Submitted (MAY-2008) to the EMBL/GenBank/DDBJ databases.
RN   [3] {ECO:0000313|EMBL:CAR67107.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=ATCC 43949 {ECO:0000313|EMBL:CAR67107.1};
RA   Thomson N.R.;
RL   Submitted (SEP-2008) to the EMBL/GenBank/DDBJ databases.
RN   [4] {ECO:0000313|EMBL:CAQ86326.1, ECO:0000313|Proteomes:UP000002747}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 43949 / 3105-77 {ECO:0000313|Proteomes:UP000002747}, and
RC   ATCC43949 {ECO:0000313|EMBL:CAQ86326.1};
RX   PubMed=19583835; DOI=10.1186/1471-2164-10-302;
RA   Wilkinson P., Waterfield N.R., Crossman L., Corton C.,
RA   Sanchez-Contreras M., Vlisidou I., Barron A., Bignell A., Clark L.,
RA   Ormond D., Mayho M., Bason N., Smith F., Simmonds M., Churcher C.,
RA   Harris D., Thompson N.R., Quail M., Parkhill J., ffrench-Constant R.H.;
RT   "Comparative genomics of the emerging human pathogen Photorhabdus
RT   asymbiotica with the insect pathogen Photorhabdus luminescens.";
RL   BMC Genomics 10:302-302(2009).
CC   -!- FUNCTION: Forms oxaloacetate, a four-carbon dicarboxylic acid source
CC       for the tricarboxylic acid cycle. {ECO:0000256|ARBA:ARBA00003670,
CC       ECO:0000256|HAMAP-Rule:MF_00595}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=oxaloacetate + phosphate = hydrogencarbonate +
CC         phosphoenolpyruvate; Xref=Rhea:RHEA:28370, ChEBI:CHEBI:16452,
CC         ChEBI:CHEBI:17544, ChEBI:CHEBI:43474, ChEBI:CHEBI:58702; EC=4.1.1.31;
CC         Evidence={ECO:0000256|ARBA:ARBA00001071, ECO:0000256|HAMAP-
CC         Rule:MF_00595};
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000256|ARBA:ARBA00001946, ECO:0000256|HAMAP-
CC         Rule:MF_00595};
CC   -!- SUBUNIT: Homotetramer. {ECO:0000256|HAMAP-Rule:MF_00595}.
CC   -!- SIMILARITY: Belongs to the PEPCase type 1 family.
CC       {ECO:0000256|ARBA:ARBA00008346, ECO:0000256|HAMAP-Rule:MF_00595}.
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DR   EMBL; FM162591; CAQ86326.1; -; Genomic_DNA.
DR   EMBL; FM211051; CAR67107.1; -; Genomic_DNA.
DR   AlphaFoldDB; B6VLS7; -.
DR   STRING; 291112.PAU_04239; -.
DR   KEGG; pay:PAU_04239; -.
DR   eggNOG; COG2352; Bacteria.
DR   Proteomes; UP000002747; Chromosome.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0008964; F:phosphoenolpyruvate carboxylase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0015977; P:carbon fixation; IEA:UniProtKB-UniRule.
DR   GO; GO:0006107; P:oxaloacetate metabolic process; IEA:UniProtKB-UniRule.
DR   GO; GO:0006099; P:tricarboxylic acid cycle; IEA:InterPro.
DR   Gene3D; 1.20.1440.90; Phosphoenolpyruvate/pyruvate domain; 1.
DR   HAMAP; MF_00595; PEPcase_type1; 1.
DR   InterPro; IPR021135; PEP_COase.
DR   InterPro; IPR022805; PEP_COase_bac/pln-type.
DR   InterPro; IPR018129; PEP_COase_Lys_AS.
DR   InterPro; IPR033129; PEPCASE_His_AS.
DR   InterPro; IPR015813; Pyrv/PenolPyrv_Kinase-like_dom.
DR   PANTHER; PTHR30523; PHOSPHOENOLPYRUVATE CARBOXYLASE; 1.
DR   PANTHER; PTHR30523:SF6; PHOSPHOENOLPYRUVATE CARBOXYLASE; 1.
DR   Pfam; PF00311; PEPcase; 1.
DR   PRINTS; PR00150; PEPCARBXLASE.
DR   SUPFAM; SSF51621; Phosphoenolpyruvate/pyruvate domain; 1.
DR   PROSITE; PS00781; PEPCASE_1; 1.
DR   PROSITE; PS00393; PEPCASE_2; 1.
PE   3: Inferred from homology;
KW   Carbon dioxide fixation {ECO:0000256|ARBA:ARBA00023300, ECO:0000256|HAMAP-
KW   Rule:MF_00595};
KW   Lyase {ECO:0000256|ARBA:ARBA00023239, ECO:0000256|HAMAP-Rule:MF_00595};
KW   Magnesium {ECO:0000256|ARBA:ARBA00022842, ECO:0000256|HAMAP-Rule:MF_00595};
KW   Pyruvate {ECO:0000313|EMBL:CAR67107.1}.
FT   ACT_SITE        137
FT                   /evidence="ECO:0000256|HAMAP-Rule:MF_00595,
FT                   ECO:0000256|PROSITE-ProRule:PRU10111"
FT   ACT_SITE        545
FT                   /evidence="ECO:0000256|HAMAP-Rule:MF_00595,
FT                   ECO:0000256|PROSITE-ProRule:PRU10112"
SQ   SEQUENCE   878 AA;  98759 MW;  F7C70837745C60FB CRC64;
     MNQQYSAMRS NVSMLGKLLG DTIKEALGEE ILDKVETIRK LSKSSRAGNE AHRQQLLSTL
     QNLSNDELLP VARAFNQFLN LTNVAEQYHS ISPHGEAASN PVALAKLFTH LKEKNFSNND
     LKKAVNELSI ELVLTAHPTE IARRTLIHKL VAVNTCLSQL DHDDLADYEH NNIMRRLRQL
     VAQSWHTDEI RKIRPTPIDE AKWGFAMVEN SLWDGVPAFL REFNEQLEES IDYSLPVEAV
     PIRFTSWMGG DRDGNPNVTA EVTRHVLLLS RWKAADLFLK DIQVLVSELS MSECTPEVRK
     LAGGDEILEP YREIAKKLRV QLNNTLAYLE KRLKGEQVLP PTDLLLDNEQ LWQPLYACYQ
     SLKICGMEII ANGQLLDILR RIRCFGLSLV RIDVRQESTR HTTAISELTQ YLELGDYASW
     SEEKKQAFLL YELHSKRPLI PHNWQPSAET QEVFATCKVI AESPQDAIAA YVISMAKAPS
     DVLAVHLLLK EASCPFTLPV APLFETLDDL NNADNVIQQL MNIKWYRALI QDKQMVMIGY
     SDSAKDAGVM AAAWAQYRAQ DALIKVCETE GVALTLFHGR GGTIGRGGAP AHAALLSQPP
     GSLKGGLRVT EQGEMIRFKF GLPQVTISSL ALYASAILEA NLLPPPEPKP EWHQVMDTLS
     DVSCKMYRDY VREQPDFVPY FRAATPEQEL AKLPLGSRPA KRRPTGGVES LRAIPWIFAW
     TQNRLMLPAW LGAGAALQKV VNDGKQDVLA EMCRNWPFFT TRIGMLEMVF AKADLWLAKY
     YDHRLVDKSL WPLGQKLRKQ LSADIKTVLA ISKDEHLMAD LPWIAESIAL RNVYTDPLNV
     LQAELLLRSR QQQYPDPQVE QALMVTIAGI AAGMRNTG
//