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B6VJS4 (ROMT_VITVI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 19. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Trans-resveratrol di-O-methyltransferase
EC=2.1.1.240
Alternative name(s):
Resveratrol O-methyltransferase
Short name=ROMT
Short name=VvROMT
Gene names
Name:ROMT
Ordered Locus Names:VIT_12s0028g01880
OrganismVitis vinifera (Grape) [Reference proteome]
Taxonomic identifier29760 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › Vitales › Vitaceae › Vitis

Protein attributes

Sequence length357 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the biosynthesis of pterostilbene from resveratrol. Pterostilbene has both antifungal and pharmacological properties. Also has activity toward resveratrol monomethyl ether (RME). Ref.1

Catalytic activity

2 S-adenosyl-L-methionine + trans-resveratrol = 2 S-adenosyl-L-homocysteine + pterostilbene. Ref.1

Induction

Expression in leaves is induced by different stresses, such as downy mildew (P.viticola) infection, ultraviolet light, and AlCl3 treatment. Ref.1

Sequence similarities

Belongs to the methyltransferase superfamily. Type 2 family. COMT subfamily.

Biophysicochemical properties

Kinetic parameters:

KM=12 µM for resveratrol Ref.1

KM=14 µM for resveratrol monomethyl ether

Ontologies

Keywords
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Molecular_functionO-methyltransferase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 357357Trans-resveratrol di-O-methyltransferase
PRO_0000418736

Sites

Active site2611Proton acceptor By similarity
Binding site2001S-adenosyl-L-methionine; via carbonyl oxygen By similarity
Binding site2231S-adenosyl-L-methionine By similarity
Binding site2431S-adenosyl-L-methionine By similarity
Binding site2441S-adenosyl-L-methionine; via amide nitrogen By similarity
Binding site2571S-adenosyl-L-methionine By similarity

Experimental info

Sequence conflict1491Y → H in CAQ76879. Ref.1
Sequence conflict2251S → P in CAQ76879. Ref.1
Sequence conflict2651N → D in CAQ76879. Ref.1

Sequences

Sequence LengthMass (Da)Tools
B6VJS4 [UniParc].

Last modified September 5, 2012. Version 2.
Checksum: D306FC2AC0F48599

FASTA35740,100
        10         20         30         40         50         60 
MDLANGVISA ELLHAQAHVW NHIFNFIKSM SLKCAIQLGI PDIIHNHGKP MTLPELVAKL 

        70         80         90        100        110        120 
PVHPKRSQCV YRLMRILVHS GFLAAQRVQQ GKEEEGYVLT DASRLLLMDD SLSIRPLVLA 

       130        140        150        160        170        180 
MLDPILTKPW HYLSAWFQND DPTPFHTAYE RSFWDYAGHE PQLNNSFNEA MASDARLLTS 

       190        200        210        220        230        240 
VLLKEGQGVF AGLNSLVDVG GGTGKVAKAI ANAFPHLNCT VLDLSHVVAG LQGSKNLNYF 

       250        260        270        280        290        300 
AGDMFEAIPP ADAILLKWIL HDWSNEECVK ILKRCREAIP SKENGGKVII IDMIMMKNQG 

       310        320        330        340        350 
DYKSTETQLF FDMTMMIFAP GRERDENEWE KLFLDAGFSH YKITPILGLR SLIEVYP

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		FM178870 mRNA. Translation: CAQ76879.1.
FN595235 Genomic DNA. Translation: CCB47386.1.
RefSeqXP_002281481.1. XM_002281445.1.
UniGeneVvi.19300.
Vvi.25952.

3D structure databases

ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID100233030.
KEGGdosa:Os01t0753300-00.
vvi:100233030.

Phylogenomic databases

KOK16040.

Enzyme and pathway databases

BioCycMetaCyc:MONOMER-16020.

Gene expression databases

ArrayExpressB6VJS4.

Family and domain databases

Gene3D1.10.10.10. 1 hit.
InterProIPR016461. COMT.
IPR001077. O_MeTrfase_2.
IPR012967. Plant_MeTrfase_dimerisation.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamPF08100. Dimerisation. 1 hit.
PF00891. Methyltransf_2. 1 hit.
[Graphical view]
PIRSFPIRSF005739. O-mtase. 1 hit.
ProtoNetSearch...

Entry information

Entry nameROMT_VITVI
AccessionPrimary (citable) accession number: B6VJS4
Secondary accession number(s): F6H518
Entry history
Integrated into UniProtKB/Swiss-Prot: September 5, 2012
Last sequence update: September 5, 2012
Last modified: May 1, 2013
This is version 19 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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