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Protein

Lipoyl synthase 1, chloroplastic

Gene

LIP1P-1

Organism
Zea mays (Maize)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.UniRule annotation

Catalytic activityi

Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine + 2 reduced [2Fe-2S] ferredoxin = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine + 2 oxidized [2Fe-2S] ferredoxin.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 2 [4Fe-4S] clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi87 – 871Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi92 – 921Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi98 – 981Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi124 – 1241Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi128 – 1281Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi131 – 1311Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. lipoate synthase activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. protein lipoylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00538; UER00593.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthase 1, chloroplastic (EC:2.8.1.8UniRule annotation)
Alternative name(s):
Lipoate synthase 1UniRule annotation
Short name:
LS 1UniRule annotation
Short name:
Lip-syn 1UniRule annotation
Lipoate synthase, plastidial 1UniRule annotation
Short name:
LIP1p 1UniRule annotation
Lipoic acid synthase 1UniRule annotation
Gene namesi
Name:LIP1P-1UniRule annotation
OrganismiZea mays (Maize)
Taxonomic identifieri4577 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaePACMAD cladePanicoideaeAndropogoneaeZea
ProteomesiUP000007305: Unplaced

Organism-specific databases

GrameneiB6TN12.

Subcellular locationi

GO - Cellular componenti

  1. chloroplast Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 361Lipoyl synthase 1, chloroplasticPRO_0000398860
Transit peptidei1 – ?ChloroplastUniRule annotation

Structurei

3D structure databases

ProteinModelPortaliB6TN12.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. Lipoyl synthase family.UniRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

HOGENOMiHOG000235998.
KOiK03644.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00206. Lipoyl_synth.
MF_03129. Lipoyl_synth_plantC.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR027526. Lipoyl_synth_chlpt.
IPR007197. rSAM.
[Graphical view]
PANTHERiPTHR10949. PTHR10949. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00510. lipA. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

B6TN12-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQSSLARPLR PPVLAGRGGR RGLVAVARCH AEAAPPVGTA SRAPAGPYTG
60 70 80 90 100
RDPEVKKPAW LRQRAAQGEK YARLRESIGE LKLNTVCVEA QCPNIGECWN
110 120 130 140 150
GGGGAGGEGD GIATATIMVL GDTCTRGCRF CAVKTSNKPP PPDPLEPLNT
160 170 180 190 200
ALAVASWGVD YVVLTSVDRD DLPDGGSSHF AQTVKALKEL KPGILVECLT
210 220 230 240 250
SDFRGDLEAI SSLASSGLDV YAHNIETVRS LQRIVRDPRA GYDQSLAVLK
260 270 280 290 300
HAKACREGMV TKSSIMLGLG ETDEEVKQAM MDLRAIGVDI LTLGQYLQPT
310 320 330 340 350
ERHLTVREYV TPEKFQFWKE YGESVGFRYV ASGPLVRSSY RAGELFVQNL
360
VRNNKTGSSS S
Length:361
Mass (Da):38,828
Last modified:December 16, 2008 - v1
Checksum:i8892DCA0C5A85299
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EU966377 mRNA. Translation: ACG38495.1.
RefSeqiNP_001150279.1. NM_001156807.1.
UniGeneiZm.23626.

Genome annotation databases

GeneIDi100283909.
KEGGizma:100283909.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EU966377 mRNA. Translation: ACG38495.1.
RefSeqiNP_001150279.1. NM_001156807.1.
UniGeneiZm.23626.

3D structure databases

ProteinModelPortaliB6TN12.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi100283909.
KEGGizma:100283909.

Organism-specific databases

GrameneiB6TN12.

Phylogenomic databases

HOGENOMiHOG000235998.
KOiK03644.

Enzyme and pathway databases

UniPathwayiUPA00538; UER00593.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00206. Lipoyl_synth.
MF_03129. Lipoyl_synth_plantC.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR027526. Lipoyl_synth_chlpt.
IPR007197. rSAM.
[Graphical view]
PANTHERiPTHR10949. PTHR10949. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00510. lipA. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

Entry informationi

Entry nameiLISC1_MAIZE
AccessioniPrimary (citable) accession number: B6TN12
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: December 16, 2008
Last modified: March 4, 2015
This is version 38 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.