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B6TN12 (LISC1_MAIZE) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 30. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Lipoyl synthase 1, chloroplastic

EC=2.8.1.8
Alternative name(s):
Lipoate synthase 1
Short name=LS 1
Short name=Lip-syn 1
Lipoate synthase, plastidial 1
Short name=LIP1p 1
Lipoic acid synthase 1
Gene names
Name:LIP1P-1
OrganismZea mays (Maize)
Taxonomic identifier4577 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaePACMAD cladePanicoideaeAndropogoneaeZea

Protein attributes

Sequence length361 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives By similarity. HAMAP-Rule MF_03129

Catalytic activity

Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_03129

Cofactor

Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Pathway

Protein modification; protein lipoylation via endogenous pathway; protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein]: step 2/2. HAMAP-Rule MF_03129

Subcellular location

Plastidchloroplast HAMAP-Rule MF_03129.

Sequence similarities

Belongs to the radical SAM superfamily. Lipoyl synthase family.

Ontologies

Keywords
   Cellular componentChloroplast
Plastid
   DomainTransit peptide
   Ligand4Fe-4S
Iron
Iron-sulfur
Metal-binding
S-adenosyl-L-methionine
   Molecular functionTransferase
Gene Ontology (GO)
   Biological_processprotein lipoylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentchloroplast

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_function4 iron, 4 sulfur cluster binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

lipoate synthase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – ?Chloroplast Potential
Chain? – 361Lipoyl synthase 1, chloroplastic HAMAP-Rule MF_03129PRO_0000398860

Sites

Metal binding871Iron-sulfur 1 (4Fe-4S) By similarity
Metal binding921Iron-sulfur 1 (4Fe-4S) By similarity
Metal binding981Iron-sulfur 1 (4Fe-4S) By similarity
Metal binding1241Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal binding1281Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal binding1311Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity

Sequences

Sequence LengthMass (Da)Tools
B6TN12 [UniParc].

Last modified December 16, 2008. Version 1.
Checksum: 8892DCA0C5A85299

FASTA36138,828
        10         20         30         40         50         60 
MQSSLARPLR PPVLAGRGGR RGLVAVARCH AEAAPPVGTA SRAPAGPYTG RDPEVKKPAW 

        70         80         90        100        110        120 
LRQRAAQGEK YARLRESIGE LKLNTVCVEA QCPNIGECWN GGGGAGGEGD GIATATIMVL 

       130        140        150        160        170        180 
GDTCTRGCRF CAVKTSNKPP PPDPLEPLNT ALAVASWGVD YVVLTSVDRD DLPDGGSSHF 

       190        200        210        220        230        240 
AQTVKALKEL KPGILVECLT SDFRGDLEAI SSLASSGLDV YAHNIETVRS LQRIVRDPRA 

       250        260        270        280        290        300 
GYDQSLAVLK HAKACREGMV TKSSIMLGLG ETDEEVKQAM MDLRAIGVDI LTLGQYLQPT 

       310        320        330        340        350        360 
ERHLTVREYV TPEKFQFWKE YGESVGFRYV ASGPLVRSSY RAGELFVQNL VRNNKTGSSS 


S 

« Hide

References

[1]"Insights into corn genes derived from large-scale cDNA sequencing."
Alexandrov N.N., Brover V.V., Freidin S., Troukhan M.E., Tatarinova T.V., Zhang H., Swaller T.J., Lu Y.-P., Bouck J., Flavell R.B., Feldmann K.A.
Plant Mol. Biol. 69:179-194(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
EU966377 mRNA. Translation: ACG38495.1.
RefSeqNP_001150279.1. NM_001156807.1.
UniGeneZm.23626.

3D structure databases

ProteinModelPortalB6TN12.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID100283909.
KEGGzma:100283909.

Organism-specific databases

GrameneB6TN12.

Phylogenomic databases

HOGENOMHOG000235998.
KOK03644.

Enzyme and pathway databases

UniPathwayUPA00538; UER00593.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_00206. Lipoyl_synth.
MF_03129. Lipoyl_synth_plantC.
InterProIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR027526. Lipoyl_synth_chlpt.
IPR007197. rSAM.
[Graphical view]
PANTHERPTHR10949. PTHR10949. 1 hit.
PfamPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFPIRSF005963. Lipoyl_synth. 1 hit.
SMARTSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00510. lipA. 1 hit.
ProtoNetSearch...

Entry information

Entry nameLISC1_MAIZE
AccessionPrimary (citable) accession number: B6TN12
Entry history
Integrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: December 16, 2008
Last modified: February 19, 2014
This is version 30 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways