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B6TN12

- LISC1_MAIZE

UniProt

B6TN12 - LISC1_MAIZE

Protein

Lipoyl synthase 1, chloroplastic

Gene

LIP1P-1

Organism
Zea mays (Maize)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 33 (01 Oct 2014)
      Sequence version 1 (16 Dec 2008)
      Previous versions | rss
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    Functioni

    Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.UniRule annotation

    Catalytic activityi

    Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

    Cofactori

    Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi87 – 871Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi92 – 921Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi98 – 981Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi124 – 1241Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi128 – 1281Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi131 – 1311Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
    2. lipoate synthase activity Source: UniProtKB-HAMAP
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. protein lipoylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Transferase

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    UniPathwayiUPA00538; UER00593.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Lipoyl synthase 1, chloroplastic (EC:2.8.1.8UniRule annotation)
    Alternative name(s):
    Lipoate synthase 1UniRule annotation
    Short name:
    LS 1UniRule annotation
    Short name:
    Lip-syn 1UniRule annotation
    Lipoate synthase, plastidial 1UniRule annotation
    Short name:
    LIP1p 1UniRule annotation
    Lipoic acid synthase 1UniRule annotation
    Gene namesi
    Name:LIP1P-1UniRule annotation
    OrganismiZea mays (Maize)
    Taxonomic identifieri4577 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaePACMAD cladePanicoideaeAndropogoneaeZea

    Organism-specific databases

    GrameneiB6TN12.

    Subcellular locationi

    GO - Cellular componenti

    1. chloroplast Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Chloroplast, Plastid

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini? – 361Lipoyl synthase 1, chloroplasticPRO_0000398860
    Transit peptidei1 – ?ChloroplastUniRule annotation

    Structurei

    3D structure databases

    ProteinModelPortaliB6TN12.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the radical SAM superfamily. Lipoyl synthase family.UniRule annotation

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    HOGENOMiHOG000235998.
    KOiK03644.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_00206. Lipoyl_synth.
    MF_03129. Lipoyl_synth_plantC.
    InterProiIPR013785. Aldolase_TIM.
    IPR006638. Elp3/MiaB/NifB.
    IPR003698. Lipoyl_synth.
    IPR027526. Lipoyl_synth_chlpt.
    IPR007197. rSAM.
    [Graphical view]
    PANTHERiPTHR10949. PTHR10949. 1 hit.
    PfamiPF04055. Radical_SAM. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
    SMARTiSM00729. Elp3. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00510. lipA. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    B6TN12-1 [UniParc]FASTAAdd to Basket

    « Hide

    MQSSLARPLR PPVLAGRGGR RGLVAVARCH AEAAPPVGTA SRAPAGPYTG    50
    RDPEVKKPAW LRQRAAQGEK YARLRESIGE LKLNTVCVEA QCPNIGECWN 100
    GGGGAGGEGD GIATATIMVL GDTCTRGCRF CAVKTSNKPP PPDPLEPLNT 150
    ALAVASWGVD YVVLTSVDRD DLPDGGSSHF AQTVKALKEL KPGILVECLT 200
    SDFRGDLEAI SSLASSGLDV YAHNIETVRS LQRIVRDPRA GYDQSLAVLK 250
    HAKACREGMV TKSSIMLGLG ETDEEVKQAM MDLRAIGVDI LTLGQYLQPT 300
    ERHLTVREYV TPEKFQFWKE YGESVGFRYV ASGPLVRSSY RAGELFVQNL 350
    VRNNKTGSSS S 361
    Length:361
    Mass (Da):38,828
    Last modified:December 16, 2008 - v1
    Checksum:i8892DCA0C5A85299
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    EU966377 mRNA. Translation: ACG38495.1.
    RefSeqiNP_001150279.1. NM_001156807.1.
    UniGeneiZm.23626.

    Genome annotation databases

    GeneIDi100283909.
    KEGGizma:100283909.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    EU966377 mRNA. Translation: ACG38495.1 .
    RefSeqi NP_001150279.1. NM_001156807.1.
    UniGenei Zm.23626.

    3D structure databases

    ProteinModelPortali B6TN12.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 100283909.
    KEGGi zma:100283909.

    Organism-specific databases

    Gramenei B6TN12.

    Phylogenomic databases

    HOGENOMi HOG000235998.
    KOi K03644.

    Enzyme and pathway databases

    UniPathwayi UPA00538 ; UER00593 .

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_00206. Lipoyl_synth.
    MF_03129. Lipoyl_synth_plantC.
    InterProi IPR013785. Aldolase_TIM.
    IPR006638. Elp3/MiaB/NifB.
    IPR003698. Lipoyl_synth.
    IPR027526. Lipoyl_synth_chlpt.
    IPR007197. rSAM.
    [Graphical view ]
    PANTHERi PTHR10949. PTHR10949. 1 hit.
    Pfami PF04055. Radical_SAM. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
    SMARTi SM00729. Elp3. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00510. lipA. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

    Entry informationi

    Entry nameiLISC1_MAIZE
    AccessioniPrimary (citable) accession number: B6TN12
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 5, 2010
    Last sequence update: December 16, 2008
    Last modified: October 1, 2014
    This is version 33 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3