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Protein

ATP-dependent 6-phosphofructokinase

Gene

PFK

Organism
Zea mays (Maize)
Status
Unreviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Enzyme regulationi

Allosterically activated by AMP.UniRule annotation

Pathwayi: glycolysis

This protein is involved in step 3 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.UniRule annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Glucose-6-phosphate isomerase, Glucose-6-phosphate isomerase, Glucose-6-phosphate isomerase, Glucose-6-phosphate isomerase, Glucose-6-phosphate isomerase, cytosolic (PHI1)
  3. ATP-dependent 6-phosphofructokinase (PFK), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit alpha (PFPalpha retrogene), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit alpha (PFP-ALPHA), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit alpha (PFP-ALPHA), ATP-dependent 6-phosphofructokinase (PFK), ATP-dependent 6-phosphofructokinase (PFK), ATP-dependent 6-phosphofructokinase (PFK), ATP-dependent 6-phosphofructokinase (PFK), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit alpha (PFP-ALPHA), ATP-dependent 6-phosphofructokinase (PFK), ATP-dependent 6-phosphofructokinase (PFK), ATP-dependent 6-phosphofructokinase (PFK), ATP-dependent 6-phosphofructokinase (PFK), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit alpha (PFP-ALPHA), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta (PFP-BETA), ATP-dependent 6-phosphofructokinase (PFK), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit alpha (PFP-ALPHA), ATP-dependent 6-phosphofructokinase (PFK), ATP-dependent 6-phosphofructokinase (PFK), ATP-dependent 6-phosphofructokinase (PFK), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit alpha (PFP-ALPHA), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta (PFP-BETA), ATP-dependent 6-phosphofructokinase (PFK), ATP-dependent 6-phosphofructokinase (PFK), ATP-dependent 6-phosphofructokinase (PFK), ATP-dependent 6-phosphofructokinase (PFK)
  4. Fructose-bisphosphate aldolase (ZEAMMB73_444541), Fructose-bisphosphate aldolase, Fructose-bisphosphate aldolase, Fructose-bisphosphate aldolase (ZEAMMB73_444541), Fructose-bisphosphate aldolase, Fructose-bisphosphate aldolase, cytoplasmic isozyme, Fructose-bisphosphate aldolase (ZEAMMB73_885208), Fructose-bisphosphate aldolase, Fructose-bisphosphate aldolase, Fructose-bisphosphate aldolase (ZEAMMB73_092309), Fructose-bisphosphate aldolase (ZEAMMB73_885208), Fructose-bisphosphate aldolase, Fructose-bisphosphate aldolase (ZEAMMB73_915833)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei142ATP; via amide nitrogenUniRule annotation1
Metal bindingi231Magnesium; catalyticUniRule annotation1
Sitei232Important for substrate specificity; cannot use PPi as phosphoryl donorUniRule annotation1
Active sitei261Proton acceptorUniRule annotation1
Binding sitei360SubstrateUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi205 – 206ATPUniRule annotation2
Nucleotide bindingi230 – 233ATPUniRule annotation4

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

KinaseUniRule annotationImported, Transferase

Keywords - Biological processi

GlycolysisUniRule annotation

Keywords - Ligandi

ATP-bindingUniRule annotation, MagnesiumUniRule annotation, Metal-bindingUniRule annotation, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinaseUniRule annotation (EC:2.7.1.11UniRule annotation)
Short name:
ATP-PFKUniRule annotation
Short name:
PhosphofructokinaseUniRule annotation
Alternative name(s):
PhosphohexokinaseUniRule annotation
Gene namesi
Name:PFKUniRule annotation
OrganismiZea mays (Maize)Imported
Taxonomic identifieri4577 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaePACMAD cladePanicoideaeAndropogonodaeAndropogoneaeTripsacinaeZea

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotation

PTM / Processingi

Proteomic databases

PaxDbiB6SWS5.
PRIDEiB6SWS5.

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi4577.GRMZM2G132069_P01.

Structurei

3D structure databases

ProteinModelPortaliB6SWS5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini136 – 440PFKInterPro annotationAdd BLAST305

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni259 – 261Substrate bindingUniRule annotation3
Regioni304 – 306Substrate bindingUniRule annotation3
Regioni417 – 420Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the phosphofructokinase type A (PFKA) family. PPi-dependent PFK group II subfamily. Atypical ATP-dependent clade "X" sub-subfamily.UniRule annotation

Phylogenomic databases

eggNOGiKOG2440. Eukaryota.
COG0205. LUCA.
KOiK00850.

Family and domain databases

HAMAPiMF_01981. Phosphofructokinase_II_X. 1 hit.
InterProiIPR022953. ATP_PFK.
IPR000023. Phosphofructokinase_dom.
IPR012004. PyroP-dep_PFK_TP0108.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000534. PPi_PFK_TP0108. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.

Sequencei

Sequence statusi: Complete.

B6SWS5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MESFGVPPAP VAAPEKRKKP LDLKDPVPAA AANGGGATKA SPAAKWAEKK
60 70 80 90 100
KLVGGDAGYV LEDVPHLTDY LPQLPTYPNP LQDNPAYSVV KQYFVNPDDT
110 120 130 140 150
VTQKIVVHKT SARGTHFRRA GPRQRVYFQP DEVNAAIVTC GGLCPGLNTV
160 170 180 190 200
IRELVCGLYD MYGVTSVVGI EGGYKGFYSK NTVALTPRSV NGIHKRGGTV
210 220 230 240 250
LGTSRGGHDT AKIVDCLQDR GINQVYIIGG DGTQKGASVI YEEVRRRGLK
260 270 280 290 300
CAVVGVPKTI DNDIAVIDKS FGFDTAVEEA QRAINAAHVE AESAENGIGV
310 320 330 340 350
VKLMGRNSGF IAMYATLASR DVDCCLIPES PFYLEGKGGL LEFAERRLKD
360 370 380 390 400
NGHMVIVVAE GAGQDLIAKS MNSVDTQDAS GNKLLLDVGL WLSQKIKDHF
410 420 430 440 450
KKKPRFPITL KYIDPTYMIR AVSSNASDNV YCTLLAHSAL HGAMAGYTGF
460 470 480 490 500
TVAPVNGRHA YIPFYRITEK QNKVVITDRM WARVLCSTNQ PCFLSHEDVE
510 520
NMKHDDDEHH LHNTLLLDSP ENSSK
Length:525
Mass (Da):57,040
Last modified:December 16, 2008 - v1
Checksum:i9D35F37C00228F73
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EU957190 mRNA. Translation: ACG29308.1.
RefSeqiNP_001147976.1. NM_001154504.1.
UniGeneiZm.118065.

Genome annotation databases

GeneIDi100281585.
KEGGizma:100281585.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EU957190 mRNA. Translation: ACG29308.1.
RefSeqiNP_001147976.1. NM_001154504.1.
UniGeneiZm.118065.

3D structure databases

ProteinModelPortaliB6SWS5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi4577.GRMZM2G132069_P01.

Proteomic databases

PaxDbiB6SWS5.
PRIDEiB6SWS5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi100281585.
KEGGizma:100281585.

Phylogenomic databases

eggNOGiKOG2440. Eukaryota.
COG0205. LUCA.
KOiK00850.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182.

Family and domain databases

HAMAPiMF_01981. Phosphofructokinase_II_X. 1 hit.
InterProiIPR022953. ATP_PFK.
IPR000023. Phosphofructokinase_dom.
IPR012004. PyroP-dep_PFK_TP0108.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000534. PPi_PFK_TP0108. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiB6SWS5_MAIZE
AccessioniPrimary (citable) accession number: B6SWS5
Entry historyi
Integrated into UniProtKB/TrEMBL: December 16, 2008
Last sequence update: December 16, 2008
Last modified: July 6, 2016
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Allosteric enzymeUniRule annotation

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.