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B6SHG7 (TRMB_MAIZE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 33. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase

EC=2.1.1.33
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
OrganismZea mays (Maize)
Taxonomic identifier4577 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaePACMAD cladePanicoideaeAndropogoneaeZea

Protein attributes

Sequence length255 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_03055

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_03055

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_03055

Subcellular location

Nucleus By similarity HAMAP-Rule MF_03055.

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Ontologies

Keywords
   Biological processtRNA processing
   Cellular componentNucleus
   LigandRNA-binding
S-adenosyl-L-methionine
tRNA-binding
   Molecular functionMethyltransferase
Transferase
Gene Ontology (GO)
   Cellular_componentnucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

tRNA binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 255255tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_03055
PRO_0000370580

Regions

Region98 – 992S-adenosyl-L-methionine binding By similarity
Region131 – 1322S-adenosyl-L-methionine binding By similarity
Region229 – 2313S-adenosyl-L-methionine binding By similarity

Sites

Active site1541 By similarity
Binding site751S-adenosyl-L-methionine; via carbonyl oxygen By similarity
Binding site1511S-adenosyl-L-methionine; via carbonyl oxygen By similarity

Sequences

Sequence LengthMass (Da)Tools
B6SHG7 [UniParc].

Last modified December 16, 2008. Version 1.
Checksum: 292FD6EE2F697E22

FASTA25529,119
        10         20         30         40         50         60 
MTRTNDASGG GKLPRKRFYR ARAHSNPLSD SHFPVPISPE EVDLSQHYPR YFPADKGSDG 

        70         80         90        100        110        120 
EEAAAPQQIR FADVGCGFGG LLVGLSPLFP DTLMIGMELR DKVTEYVKER ILALRGANPG 

       130        140        150        160        170        180 
QYDNISVVRT NSMKYIPNYF RKAQLTKMFF LFPDPHFKEK NHRRRVISMQ LLDEYAYVME 

       190        200        210        220        230        240 
VGGIIYTITD VEELGEWMRS CLEKHPLFET VPEEEIKADP VFKLLSTATE ESQKVARNGG 

       250 
QTFYAIFRRI SLQEE 

« Hide

References

[1]"Insights into corn genes derived from large-scale cDNA sequencing."
Alexandrov N.N., Brover V.V., Freidin S., Troukhan M.E., Tatarinova T.V., Zhang H., Swaller T.J., Lu Y.-P., Bouck J., Flavell R.B., Feldmann K.A.
Plant Mol. Biol. 69:179-194(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[2]"Maize full-length cDNA project."
Yu Y., Currie J., Lomeli R., Angelova A., Collura K., Wissotski M., Campos D., Kudrna D., Golser W., Ashely E., Haller K., Descour A., Fernandes J., Zuccolo A., Soderlund C., Walbot V.
Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. B73.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
EU952182 mRNA. Translation: ACG24300.1.
BT066355 mRNA. Translation: ACN33252.1.
RefSeqNP_001146900.1. NM_001153428.1.
UniGeneZm.27927.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID100280508.
KEGGzma:100280508.

Organism-specific databases

GrameneB6SHG7.

Phylogenomic databases

HOGENOMHOG000260965.
KOK03439.
OMARAHSNPI.

Enzyme and pathway databases

UniPathwayUPA00989.

Family and domain databases

HAMAPMF_03055. tRNA_methyltr_TrmB_euk.
InterProIPR025763. Trm8_euk.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
TIGRFAMsTIGR00091. TIGR00091. 1 hit.
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_MAIZE
AccessionPrimary (citable) accession number: B6SHG7
Entry history
Integrated into UniProtKB/Swiss-Prot: May 26, 2009
Last sequence update: December 16, 2008
Last modified: April 16, 2014
This is version 33 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways