Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene
N/A
Organism
Zea mays (Maize)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei75 – 751S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation
Binding sitei151 – 1511S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation
Active sitei154 – 1541UniRule annotation

GO - Molecular functioni

  1. tRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-HAMAP
  2. tRNA binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. nucleotide biosynthetic process Source: EnsemblPlants/Gramene
  2. protein targeting to mitochondrion Source: EnsemblPlants/Gramene
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

RNA-binding, S-adenosyl-L-methionine, tRNA-binding

Enzyme and pathway databases

UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
OrganismiZea mays (Maize)
Taxonomic identifieri4577 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaePACMAD cladePanicoideaeAndropogoneaeZea
ProteomesiUP000007305 Componenti: Chromosome 4

Organism-specific databases

GrameneiB6SHG7.

Subcellular locationi

  1. Nucleus UniRule annotation

GO - Cellular componenti

  1. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 255255tRNA (guanine-N(7)-)-methyltransferasePRO_0000370580Add
BLAST

Expressioni

Gene expression databases

ExpressionAtlasiB6SHG7. baseline.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni98 – 992S-adenosyl-L-methionine bindingUniRule annotation
Regioni131 – 1322S-adenosyl-L-methionine bindingUniRule annotation
Regioni229 – 2313S-adenosyl-L-methionine bindingUniRule annotation

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000260965.
KOiK03439.
OMAiMCTHFEE.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_03055. tRNA_methyltr_TrmB_euk.
InterProiIPR029063. SAM-dependent_MTases.
IPR025763. Trm8_euk.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B6SHG7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTRTNDASGG GKLPRKRFYR ARAHSNPLSD SHFPVPISPE EVDLSQHYPR
60 70 80 90 100
YFPADKGSDG EEAAAPQQIR FADVGCGFGG LLVGLSPLFP DTLMIGMELR
110 120 130 140 150
DKVTEYVKER ILALRGANPG QYDNISVVRT NSMKYIPNYF RKAQLTKMFF
160 170 180 190 200
LFPDPHFKEK NHRRRVISMQ LLDEYAYVME VGGIIYTITD VEELGEWMRS
210 220 230 240 250
CLEKHPLFET VPEEEIKADP VFKLLSTATE ESQKVARNGG QTFYAIFRRI

SLQEE
Length:255
Mass (Da):29,119
Last modified:December 16, 2008 - v1
Checksum:i292FD6EE2F697E22
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EU952182 mRNA. Translation: ACG24300.1.
BT066355 mRNA. Translation: ACN33252.1.
RefSeqiNP_001146900.1. NM_001153428.1.
XP_008677164.1. XM_008678942.1.
XP_008677165.1. XM_008678943.1.
XP_008677166.1. XM_008678944.1.
UniGeneiZm.27927.

Genome annotation databases

EnsemblPlantsiGRMZM2G019597_T01; GRMZM2G019597_P01; GRMZM2G019597.
GRMZM2G019597_T02; GRMZM2G019597_P02; GRMZM2G019597.
GRMZM2G019597_T03; GRMZM2G019597_P03; GRMZM2G019597.
GeneIDi100280508.
KEGGizma:100280508.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EU952182 mRNA. Translation: ACG24300.1.
BT066355 mRNA. Translation: ACN33252.1.
RefSeqiNP_001146900.1. NM_001153428.1.
XP_008677164.1. XM_008678942.1.
XP_008677165.1. XM_008678943.1.
XP_008677166.1. XM_008678944.1.
UniGeneiZm.27927.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiGRMZM2G019597_T01; GRMZM2G019597_P01; GRMZM2G019597.
GRMZM2G019597_T02; GRMZM2G019597_P02; GRMZM2G019597.
GRMZM2G019597_T03; GRMZM2G019597_P03; GRMZM2G019597.
GeneIDi100280508.
KEGGizma:100280508.

Organism-specific databases

GrameneiB6SHG7.

Phylogenomic databases

HOGENOMiHOG000260965.
KOiK03439.
OMAiMCTHFEE.

Enzyme and pathway databases

UniPathwayiUPA00989.

Gene expression databases

ExpressionAtlasiB6SHG7. baseline.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_03055. tRNA_methyltr_TrmB_euk.
InterProiIPR029063. SAM-dependent_MTases.
IPR025763. Trm8_euk.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  2. "Maize full-length cDNA project."
    Yu Y., Currie J., Lomeli R., Angelova A., Collura K., Wissotski M., Campos D., Kudrna D., Golser W., Ashely E., Haller K., Descour A., Fernandes J., Zuccolo A., Soderlund C., Walbot V.
    Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. B73.

Entry informationi

Entry nameiTRMB_MAIZE
AccessioniPrimary (citable) accession number: B6SHG7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 26, 2009
Last sequence update: December 16, 2008
Last modified: April 1, 2015
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.