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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene
N/A
Organism
Zea mays (Maize)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathwayi: N(7)-methylguanine-tRNA biosynthesis

This protein is involved in the pathway N(7)-methylguanine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway N(7)-methylguanine-tRNA biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei75S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1
Binding sitei151S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1
Active sitei154UniRule annotation1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

RNA-binding, S-adenosyl-L-methionine, tRNA-binding

Enzyme and pathway databases

UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
OrganismiZea mays (Maize)
Taxonomic identifieri4577 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaePACMAD cladePanicoideaeAndropogonodaeAndropogoneaeTripsacinaeZea
Proteomesi
  • UP000007305 Componenti: Unplaced

Subcellular locationi

  • Nucleus UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003705801 – 255tRNA (guanine-N(7)-)-methyltransferaseAdd BLAST255

Proteomic databases

PaxDbiB6SHG7.

Expressioni

Gene expression databases

ExpressionAtlasiB6SHG7. baseline and differential.

Interactioni

Protein-protein interaction databases

STRINGi4577.GRMZM2G019597_P01.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni98 – 99S-adenosyl-L-methionine bindingUniRule annotation2
Regioni131 – 132S-adenosyl-L-methionine bindingUniRule annotation2
Regioni229 – 231S-adenosyl-L-methionine bindingUniRule annotation3

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

eggNOGiKOG3115. Eukaryota.
COG0220. LUCA.
HOGENOMiHOG000260965.
KOiK03439.
OMAiPNFFYKG.
OrthoDBiEOG09360OC8.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_03055. tRNA_methyltr_TrmB_euk. 1 hit.
InterProiIPR029063. SAM-dependent_MTases.
IPR025763. Trm8_euk.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B6SHG7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTRTNDASGG GKLPRKRFYR ARAHSNPLSD SHFPVPISPE EVDLSQHYPR
60 70 80 90 100
YFPADKGSDG EEAAAPQQIR FADVGCGFGG LLVGLSPLFP DTLMIGMELR
110 120 130 140 150
DKVTEYVKER ILALRGANPG QYDNISVVRT NSMKYIPNYF RKAQLTKMFF
160 170 180 190 200
LFPDPHFKEK NHRRRVISMQ LLDEYAYVME VGGIIYTITD VEELGEWMRS
210 220 230 240 250
CLEKHPLFET VPEEEIKADP VFKLLSTATE ESQKVARNGG QTFYAIFRRI

SLQEE
Length:255
Mass (Da):29,119
Last modified:December 16, 2008 - v1
Checksum:i292FD6EE2F697E22
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EU952182 mRNA. Translation: ACG24300.1.
BT066355 mRNA. Translation: ACN33252.1.
RefSeqiNP_001146900.1. NM_001153428.1.
XP_008677164.1. XM_008678942.1.
XP_008677165.1. XM_008678943.1.
XP_008677166.1. XM_008678944.1.
UniGeneiZm.27927.

Genome annotation databases

EnsemblPlantsiZm00001d053963_T001; Zm00001d053963_T001; Zm00001d053963.
Zm00001d053963_T002; Zm00001d053963_T002; Zm00001d053963.
Zm00001d053963_T003; Zm00001d053963_T003; Zm00001d053963.
GeneIDi100280508.
GrameneiZm00001d053963_T001; Zm00001d053963_T001; Zm00001d053963.
Zm00001d053963_T002; Zm00001d053963_T002; Zm00001d053963.
Zm00001d053963_T003; Zm00001d053963_T003; Zm00001d053963.
KEGGizma:100280508.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EU952182 mRNA. Translation: ACG24300.1.
BT066355 mRNA. Translation: ACN33252.1.
RefSeqiNP_001146900.1. NM_001153428.1.
XP_008677164.1. XM_008678942.1.
XP_008677165.1. XM_008678943.1.
XP_008677166.1. XM_008678944.1.
UniGeneiZm.27927.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi4577.GRMZM2G019597_P01.

Proteomic databases

PaxDbiB6SHG7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiZm00001d053963_T001; Zm00001d053963_T001; Zm00001d053963.
Zm00001d053963_T002; Zm00001d053963_T002; Zm00001d053963.
Zm00001d053963_T003; Zm00001d053963_T003; Zm00001d053963.
GeneIDi100280508.
GrameneiZm00001d053963_T001; Zm00001d053963_T001; Zm00001d053963.
Zm00001d053963_T002; Zm00001d053963_T002; Zm00001d053963.
Zm00001d053963_T003; Zm00001d053963_T003; Zm00001d053963.
KEGGizma:100280508.

Phylogenomic databases

eggNOGiKOG3115. Eukaryota.
COG0220. LUCA.
HOGENOMiHOG000260965.
KOiK03439.
OMAiPNFFYKG.
OrthoDBiEOG09360OC8.

Enzyme and pathway databases

UniPathwayiUPA00989.

Gene expression databases

ExpressionAtlasiB6SHG7. baseline and differential.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_03055. tRNA_methyltr_TrmB_euk. 1 hit.
InterProiIPR029063. SAM-dependent_MTases.
IPR025763. Trm8_euk.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTRMB_MAIZE
AccessioniPrimary (citable) accession number: B6SHG7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 26, 2009
Last sequence update: December 16, 2008
Last modified: November 30, 2016
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.